Lafontaine, Daniel

Professeur, Faculté des sciences
FAC. SCIENCES Biologie

Coordonnées

Courriel


819-821-8000, poste 65011


Site Web

Diplômes

(2002) Postdoctorat (Biochimie structurale). University of Dundee.

(1999) Doctorat (Doctorat en biochimie). Université de Sherbrooke.

(1994) Baccalauréat (Baccalauréat en biochimie). Université de Sherbrooke.

Présentation

Sujets de recherche

Expression et régulation génique, Génétique moléculaire, Infections bactériennes, Antibiotiques et résistance.

Disciplines de recherche

Biochimie, Biologie moléculaire.

Mots-clés

Catalyse par l'ARN, Enzymologie, FRET, Ribozymes, Structure de l'ARN, Thermodynamique.

Intérêts de recherche

Comprendre les mécanismes impliqués dans la formation de structures tertiaires adoptées par les macromolécules. Notre recherche vise aussi le développement de nouveaux types d'antibiotiques ciblant les riborégulateurs.

Centres de recherche

Aucun, Centre de recherche du CHUS.

Langues parlées et écrites

Anglais, Français

Prix et distinctions

  • (2011) FRQS Scholar Junior II, Salary Award. Fonds de recherche du Québec - Santé (FRQS). (Prix / Récompense).
  • (2011) CIHR New Investigator Award. Instituts de recherche en santé du Canada. (Prix / Récompense).
  • (1998) EMBO Practical Course (Univ. Copenhagen). EMBO. (Distinction).
  • "Prix Relève" given by the Fondation du CHUS/Royal Bank of Canada given to the most promising young researcher. Centre Hospitalier Univ. de Sherbrooke. (Prix / Récompense).
  • Accelerated promotion to the Professor rank (attributed only to outstanding candidates). Université de Sherbrooke. (Distinction).
  • Alumnus of the Year 2010 (Riboclub organization). Ribo-Club. (Distinction).
  • Best Research Discovery in Quebec in 2010 according to a public vote. Québec Science. (Distinction).
  • FRQS Scholar Senior. Fonds de recherche du Québec - Santé (FRQS). (Prix / Récompense).
  • Mérite Estrien (Attributed to people having a high research impact in society). La Tribune / Radio-Canada. (Distinction).
  • Part of the Top 10 Research Discoveries in 2010 according to Québec Science. Québec Sciences. (Distinction).
  • Prix Roche (Best scientific achievements during PhD studies). Roche Diagnostic. (Distinction).

Financement

Subvention. (En cours d’évaluation). Demandeur principal. Study of riboswitch regulatory mechanisms in Escherichia coli. Conseil de Recherches en Sciences Naturelles et Génie du Canada (CRSNG). 36000 $ (2020-2025).

Subvention. (Obtenu). Demandeur principal. Real-time imaging and co-transcriptional probing of riboswitch regulatory mechanisms. Instituts de Recherche en Santé du Canada (IRSC). Operating grant. 699975 $ (2017-2022).

Subvention. (Obtenu). Collaborateur. Exploration of the TPP riboswitch as a new target for antibiotics. JPIAMR. Joint Programming Initiative on Antimicrobial Resistance. 1782500 $ (2019-2021).

Subvention. (Obtenu). Chercheur principal. Characterization of transcriptional pausing data. Université de Sherbrooke, Faculté des sciences. Aide à la Recherche: Soutien facultaire au recrutement aux études supérieures. 18000 $ (2019-2020).

Subvention. (Obtenu). Demandeur principal. Importance of transcriptional pausing in bacterial riboswitch regulation. Conseil de Recherches en Sciences Naturelles et Génie du Canada (CRSNG). 36000 $ (2019-2020).

Subvention. (Obtenu). Chercheur principal. Deciphering TPP recognition by a bacterial riboswitch in real-time. Université de Sherbrooke, Faculté des sciences. Aide à la Recherche: Soutien facultaire au recrutement aux études supérieures. 66000 $ (2017-2019).

Subvention. (Obtenu). Chercheur principal. The role of metabolite binding on transcriptional pausing in E. coli riboswitches. Université de Sherbrooke, Faculté des sciences. Aide à la Recherche: Soutien facultaire au recrutement aux études supérieures. 66000 $ (2017-2019).

Subvention. (Obtenu). Chercheur principal. Studying the role of transcription factors on transcriptionnal pauses. Université de Sherbrooke, Faculté des sciences. Aide à la Recherche: Soutien facultaire au recrutement aux études supérieures. 22000 $ (2018).

Publications

Articles de revue

  • Chauvier A*, Nadon JF*, Grondin JP*, Lamontagne AM*, Lafontaine DA. (2019). Role of a hairpin-stabilized pause in the Escherichia coli thiC riboswitch function. RNA Biology, 16(8), 1066-1073. (Article publié).
  • McCluskey K, Boudreault J*, St-Pierre P*, Perez-Gonzalez C, Chauvier A*, Rizzi A, Beauregard PB, Lafontaine DA, Penedo JC. (2019). Unprecedented tunability of riboswitch structure and regulatory function by sub-millimolar variations in physiological Mg2. Nucleic Acids Res, 47(12), 6478-6487. (Article publié).
  • Bastet L*, Turcotte P*, Wade JT, Lafontaine DA. (2018). Maestro of regulation: Riboswitches orchestrate gene expression at the levels of translation, transcription and mRNA decay. RNA Biol, 15, 679-682. (Article publié).
  • Yan LH, Le Roux A, Boyapelly K, Lamontagne AM*, Archambault MA*, Picard-Jean F*, Lalonde-Seguin D, St-Pierre E*, Najmanovich RJ, Fortier LC, Lafontaine DA, Marsault E. (2018). Purine analogs targeting the guanine riboswitch as potential antibiotics against Clostridioides difficile. Eur J Med Chem, 143, 755-768. (Article publié).
  • Laterreur N, Lemieux B, Neumann H, Berger-Dancause JC*, Lafontaine D, Wellinger RJ. (2018). The yeast telomerase module for telomere recruitment requires a specific RNA architecture. RNA, 24, 1067-1079. (Article publié).
  • Chauvier A*, Picard-Jean F*, Berger-Dancause JC*, Bastet L*, Naghdi MR, Dube A*, Turcotte P*, Perreault J, Lafontaine DA. (2017). Nature Communications, 8, 13892. (Article publié).
  • Dussault AM*, Dubé A*, Jacques F*, Grondin JP*, Lafontaine DA. (2017). Ligand Recognition and Helical Stacking Formation are Intimately Linked in the SAM-I Riboswitch Regulatory Mechanism. Nucleic Acids Res, 23, 1539-1551. (Article publié).
  • Bastet L*, Chauvier A*, Singh N, Lussier A*, Lamontagne AM, Prevost K, Masse E, Wade JT, Lafontaine DA. (2017). Translational control and Rho-dependent transcription termination are intimately linked in riboswitch regulation. Nucleic Acids Res, 45, 7474-7486. (Article publié).
  • Perez-Gonzalez C, Lafontaine DA°, Penedo JC° (°corresponding, review article). (2016). Fluorescence-Based Strategies to Investigate the Structure and Dynamics of Aptamer-Ligand Complexes. Frontiers in chemistry, 4, 33-55. (Article publié).
  • Comeau MA*, Lafontaine DA°, Abou Elela S° (°corresponding). (2016). The catalytic efficiency of yeast ribonuclease III depends on substrate specific product release rate. Nucleic Acids Res, 44, 7911-7921. (Article publié).
  • Lussier A*, Bastet L*, Chauvier A*, Lafontaine DA. (2015). A kissing-loop is important for BtuB riboswitch ligand sensing and regulatory control. J Biol Chem, 290, 26739-26751. (Article publié).
  • Bordeleau E, Purcell EB, Lafontaine DA, Fortier LC, Tamayo R, Burrus V. (2015). Cyclic di-GMP riboswitch-regulated type IV pili contribute to aggregation of Clostridium difficile. J Bacteriol, 197, 819-832. (Article publié).
  • Smith-Peter E*, Lamontagne AM, Lafontaine DA. (2015). Role of lysine binding residues in the global folding of the lysC riboswitch. RNA Biol, 12, 1372-1382. (Article publié).
  • St-Pierre P*, McCluskey K, Shaw E, Penedo JC°, Lafontaine DA° (°corresponding, review article). (2014). Fluorescence tools to investigate riboswitch structural dynamics. Biochim Biophys Acta, 1839, 1005-1019. (Article publié).
  • Laterreur N, Eschbach S*, Lafontaine DA, Wellinger R. (2013). A new telomerase RNA element that is critical for telomere elongation. Nucleic Acids Res, 41, 7713-24. (Article publié).
  • Ster C, Allard M, Boulanger S, Lamontagne Boulet M, Mulhbacher J*, Lafontaine DA, Marsault E, Lacasse P, Malouin M. (2013). Experimental treatment of Staphylococcus aureus bovine intramammary infection using a guanine riboswitch ligand analog. Journal of Dairy Science, 96, 1000-8. (Article publié).
  • Chinnappan R*, Dubé A*, Lemay JF*, Lafontaine DA. (2013). Fluorescence monitoring of riboswitch transcription regulation using a dual molecular beacon assay. Nucleic Acids Res, 41, e106. (Article publié).
  • Lalaouna D, Simoneau-Roy M*, Lafontaine DA, Massé E (review article). (2013). Regulatory RNAs and target mRNA decay in prokaryotes. BBA - Gene Regulatory Mechanisms, 1829, 742-7. (Article publié).
  • Dalgarno P, Bordello J, Morris R, St-Pierre P*, Dubé A*, Samuel L*, Lafontaine DA°, Penedo JC°(°corresponding) "Top 5% of papers in terms of originality, significance and scientific excellence". (2013). Single-molecule denaturation of riboswitches. Nucleic Acids Res, 41, 4253-65. (Article publié).
  • Caron MP*, Bastet L*, Lussier A*, Simoneau-Roy M*, Massé E°, Lafontaine DA° (°corresponding). "Selected by Faculty of 1000 and was highlighted in Nature Reviews Microbiology". (2012). Dual-acting riboswitch control of translation initiation and mRNA decay. Proc Natl Acad Sci USA, 109, e3444-53. (Article publié).
  • Eschbach SH*, St-Pierre P*, Penedo JC°, Lafontaine DA° (°corresponding, review article). (2012). Folding of the SAM-I riboswitch: A tale with a twist. RNA Biology, 9, 535-41. (Article publié).
  • Lemay JF*, Desnoyers G, Blouin S*, Heppell B*, Bastet L*, St-Pierre P*, Massé E°, Lafontaine DA° (°corresponding). (2011). Comparative study between transcriptionally- and translationally-acting adenine riboswitches reveals key differences in riboswitch regulatory mechanisms. PLoS Genetics, 7, e1001278. (Article publié).
  • Tremblay R*, Lemay JF*, Blouin S*, Mulhbacher J*, Bonneau E, Legault P, Dupont P, Penedo JC, Lafontaine DA. (2011). Constitutive regulatory activity of an evolutionary-excluded riboswitch variant. The Journal of Biological Chemistry, 286, 27406-15. (Article publié).
  • Blouin S*, Chinnappan R*, Lafontaine DA. (2011). Folding of the lysine riboswitch: importance of peripheral elements for transcriptional regulation. Nucleic Acids Res, 39, 3373-81. (Article publié).
  • Heppell B*, Blouin S*, Dussault AM*, Mulhbacher J*, Ennifar E, Penedo JC°, Lafontaine DA°. (°corresponding). "Selected for the news and views of the same journal and was selected by Falculty of 1000.". (2011). Molecular insights into the ligand-controlled organization of the SAM-I riboswitch. Nature Chemical Biology, 7, 384-868. (Article publié).
  • Bastet L*, Dubé A*, Massé E, Lafontaine DA (review article). (2011). New insights into riboswitch regulation mechanisms. Molecular Microbiology, 80, 1148-54. (Article publié).
  • Mulhbacher J*, Brouillette E, Allard M, Fortier LC, Malouin F°, Lafontaine DA° (°corresponding). "Mentionned in Research highlighs of Nature Chemical Biology". (2010). Novel riboswitch ligand analogs as selective inhibitors of guanine-related metabolic pathways. PLoS Pathogens, 6(4), e1000865-e1000865. (Article publié).
  • Delfosse V, Bouchard P, Bonneau E, Dagenais P, Lemay JF*, Lafontaine DA°, Legault P° (°corresponding) "Selected by Faculty of 1000". (2010). Riboswitch structure: an internal residue mimicking the purine ligand. Nucleic Acids Res, 38, 32057-68. (Article publié).
  • Caron MP*, Lafontaine DA, Massé E (review article) "Selected as the Most popular issue of the month". (2010). Small RNA-mediated regulation at the level of transcript stability. RNA Biol, 7, 140-4. (Article publié).
  • Mulhbacher J*, St-Pierre P*, Lafontaine DA (review article). (2010). Therapeutic applications of ribozymes and riboswitches. Current Opinion in Pharmacology, 10(5), 551-556. (Article publié).
  • Blouin S*, Mulhbacher J*, Penedo JC°, Lafontaine DA° (°corresponding, review article). (2009). Riboswitches: Ancient and Promising Genetic Regulators. ChemBioChem, 10, 400-16. (Article publié).
  • Heppell B*, Lafontaine DA. (2008). Folding of the SAM Aptamer is Determined by the Formation of a K-turn-dependent Pseudoknot. Biochemistry, 47, 1490-9. (Article publié).
  • Blouin S*, Lafontaine DA. (2007). A loop loop interaction and a K-turn motif located in the lysine aptamer domain are important for the riboswitch gene regulation control. RNA, 13(8), 1256-67. (Article publié).
  • Lemay JF*, Lafontaine DA. (2007). Core requirements of the adenine riboswitch aptamer for ligand binding. RNA, 13(3), 339-50. (Article publié).
  • Mulhbacher J* , Lafontaine DA. (2007). Ligand recognition determinants of guanine riboswitches. Nucleic acids research, 35(16), 5568-80. (Article publié).
  • Lemay JF*, Penedo JC, Tremblay R*, Lilley DM, Lafontaine DA. (2006). Folding of the adenine riboswitch. Chemistry & biology, 13(8), 857-68. (Article publié).

Livres

  • Singh N, Lange M J, Scarborough R J, Jiang X, Carter J R, D’Anjou F, Lee S-W, Bajaj P, Korbi A, Daldrop P, Colizzi F, Tripp V T, Pedrolli D B, Fowler C C, Lünse C E, Baird N J, Soulière M F, Yan A, Thomas J M et al. (2014). Therapeutic Applications of Ribozymes and Riboswitches - Methods in Molecular Biology (1103). États-Unis : Humana Press. (Article publié).

Chapitres de livre

  • Perez-Gonzalez C, Grondin JP*, Lafontaine DA, Carlos Penedo J. (2016). Biophysical Approaches to Bacterial Gene Regulation by Riboswitches. Leake M C Biophysics of Infection (157-191). États-Unis : Springer International Publishing. (Article publié).
  • Blouin S*, Craggs TD, Lafontaine DA, Penedo JC. (2015). Functional studies of DNA-protein interactions using FRET techniques. Leblanc B P, Rodrigue S Methods Mol Biol (115-141). États-Unis : Humana Press. (Article publié).
  • Chauvier A*, Lafontaine DA. (2015). Probing of nascent riboswitch transcripts. Leblanc B P, Rodrigue S Methods Mol Biol (109-114). États-Unis : Humana Press. (Article publié).
  • Colizzi F, Lamontagne AM*, Lafontaine DA, Bussi G. (2015). Probing riboswitch binding sites with molecular docking, focused libraries, and in-line probing assays. Lafontaine D A, Dubé A Methods Mol Biol (141-151). États-Unis : Humana Press. (Article publié).
  • Boudreault J*, Perez-Gonzalez DC, Penedo JC, Lafontaine DA. (2015). Single-molecule approaches for the characterization of riboswitch folding mechanisms. Leblanc B P, Rodrigue S Methods Mol Biol (101-107). États-Unis : Humana Press. (Article publié).
  • Eschbach SH*, Lafontaine DA. (2014). RNA conformational changes analyzed by comparative gel electrophoresis. Waldsich C Methods Mol Biol (255-265). États-Unis : Humana Press. (Article publié).
  • McCluskey K, Shaw E, Lafontaine DA, Penedo JC. (2014). Single-molecule fluorescence of nucleic acids. Engelborghs Y, Visser A J W G Methods Mol Biol (759-791). États-Unis : Humana Press. (Article publié).
  • Shaw E, St-Pierre P*, McCluskey K, Lafontaine DA, Penedo JC. (2014). Using sm-FRET and denaturants to reveal folding landscapes. Pecoraro V L Methods Enzymol (313-341). Pays-Bas : Elsevier. (Article publié).
  • Heppell B*, Mulhbacher J*, Penedo JC, Lafontaine DA. (2009). Application of Fluorescence Measurements for Characterization of Riboswitch-Ligand Interactions. Serganov A Methods Mol Biol (25-37). États-Unis : Humana Press. (Article publié).
  • Blouin S*, Craggs T, Lafontaine DA, Penedo JC. (2009). Functional studies of DNA-protein interactions using FRET techniques. Leblanc B, Moss T Methods Mol Biol (475-502). États-Unis : Humana Press. (Article publié).
  • Lemay JF*, Mulhbacher J*, Penedo JC, Lafontaine DA. (2009). Molecular Basis of RNA-Mediated Gene Regulation on the Adenine Riboswitch by Single-Molecule Approaches. Serganov A Methods Mol Biol (65-76). États-Unis : Humana Press. (Article publié).

Documents de travail

  • St-Pierre P, Grondin J and Lafontaine DA. (2016). Folding of the adenine riboswitch core domain monitored at the singlemolecule level.
  • St-Pierre P, Jacques S, Picard-Jean F, Penedo J C and Lafontaine DA. (2016). Folding pathway of the add adenine riboswitch monitored using singlemolecule FRET.
  • Nadon JF, Lafontaine DA. (2016). Influence of NPOM-dT photocaged DNA on transcription by E. coli and T7 RNA polymerases.
  • Dussault AM*, Dubé A, Jacques F and Lafontaine DA. (2016). Ligand Recognition and Helical Stacking Formation are Intimately Linked in the SAM-I Riboswitch Regulatory Mechanism.
  • Bastet L, Chauvier A, Singh N, Lussier A, Wade J and Lafontaine DA. (2016). Riboswitch-mediated translation repression directs Rho termination and mRNA decay through a single RNA element.
  • Boudreault J, Perez-Gonzalez DC, Heppell B, Penedo C and Lafontaine DA. (2016). SAM-I riboswitch mutagenesis reveals clues on a unique ligand dependent stem rotation mechanism at single-molecule level.
  • Bastet L, Lamontagne AM, Massé E and Lafontaine DA. (2016). Small RNA and riboswitch btuB translation inhibition modulate mRNA levels through different mechanisms.

Articles de conférence

  • Hien E*, Michaud A*, Vanutelli A, Vezina-Bedard AS*, Yague C. (2019). L'ARN, bien plus qu'un messager. JVSS 2019 ****Best poster presentation and prize from the public****. (Article accepté).
  • Lamontagne AM*, St-Pierre P*, Lafontaine DA. (2019). RNA polymerase walking on photocleavable solid phase for sm-FRET analysis of RNA folding. Riboclub 2019. (Article accepté).
  • Grondin JP*,Chauvier A*, St-Pierre P*, Nadon JF*, Turcotte P*, Geffroy M*, Perez-Gonzalez C, Eschbach SH*, Simoneau-Roy M*, Lamontagne AM*, Massé E, Penedo JC, Lafontaine DA. (2019). Real time imaging of co-transcriptional folding and translation regulation. RNA 2019. (Article accepté).
  • Nadon JF*, Epshtein V, Nudler E, Lafontaine DA. (2019). Shedding light on transcriptional pausing: A new photolabile roadblock allows more reliable studies of elongation dynamics. Riboclub 2019. (Article accepté).
  • Chauvier A*, St-Pierre P*, Nadon JF*, Geffroy M*, Perez-Gonzalez C, Eschbach SH*, Simoneau-Roy M*, Lamontagne AM*, Masse E, Penedo JC, Lafontaine DA. (2019). Single-molecule FRET imaging of riboswitch transcriptional complexes. RNA 2019. (Article accepté).
  • Hien E*, Chauvier A*, Nadon JF*, Lafontaine DA. (2019). Study of thiC riboswitch transcriptional pauses in Escherichia coli. Canadian Society of Microbiology 2019. (Article accepté).
  • Hien E*, Chauvier A*, Nadon JF*, Lafontaine DA. (2019). Study of thiC riboswitch transcriptional pauses in Escherichia coli. Riboclub 2019. (Article accepté).
  • Vézina Bédard AS*, Geffroy M*, Martin F, Singh N, Massé É, Wade J, Guillier M, Lafontaine DA. (2019). corA : a new riboswitch in Escherichia coli. RNA 2019. (Article accepté).
  • Vézina Bédard AS*, Geffroy M*, Martin F, Singh N, Massé É, Wade J, Guillier M, Lafontaine DA. (2019). corA : a new riboswitch in Escherichia coli. Riboclub 2019. (Article accepté).
  • Nadon JF*, Lafontaine DA. (2018). Development of a light-dependent transcriptional roadblock. Riboclub 2018. (Article accepté).
  • Grondin J*, Lafontaine DA. (2018). Monitoring the 30S binding dynamics to a tbpA riboswitch in real time. Riboclub 2018. (Article accepté).
  • Grondin J*, St-Pierre P, Lamontagne AM, Lafontaine DA. (2018). Monitoring the 30S binding dynamics to a tbpA riboswitch in real time. RNA 2018. (Article accepté).
  • Berger-Dancause JF*, Hussein S, Lafontaine DA. (2018). Structural study of the TUNAR RNA. Riboclub 2018. (Article accepté).
  • Michaud A*, Lafontaine DA. (2018). Study of thiM mRNA levels in E coli using real-time fluorescence assays. Riboclub 2018. (Article accepté).
  • Vezina-Bedard AS*, Geffroy M*, Guillier M, Lafontaine DA. (2018). corA : A new riboswitch in Escherichia coli?. Riboclub 2018. (Article accepté).
  • Turcotte P*, Chauvier A*, Bastet L*, Singh N, Wade JT, Lafontaine DA. (2017). A genetic regulation hotspot in the lysC riboswitch. Riboclub 2017. (Article publié).
  • Michaud A*, Chauvier A*, Lafontaine DA, Fortier LC. (2017). The glycine riboswitches in Clostridium difficile: a path to explore. Riboclub 2017. (Article accepté).
  • Nadon JF*, Lafontaine DA. (2017). Transcriptional Traffic Lights: Using photochemistry to control RNA elongation. Riboclub 2017. (Article accepté).
  • Geffroy M*, Stringer AM, Prevost K, Masse E, Lafontaine DA. (2017). mgtAThe riboswitch uses leader peptidetranslation to regulate gene expression in. Riboclub 2017. (Article publié).
  • Grondin JP*, Lafontaine DA. (2017). tbpAMonitoringthe 30S binding dynamics to a riboswitchin real time. Riboclub 2017. (Article publié).
  • Grondin JP*, St-Pierre P*, Lafontaine D. (2016). Deciphering TPP recognition by a bacterial riboswitch in real-time. Riboclub 2016. (Article publié).
  • Chauvier A*, Picard-Jean F, Berger-Dancause JC*, Dubé A*, Naghbi R, Perreault J, Lafontaine D. (2016). Folding and regulation mechanism of the thiC riboswitch. RNA meeting 2016. (Article publié).
  • Turcotte P*, Chauvier A*, Bastet L*, Singh N, Caron MP*, Wade JT, Lafontaine DA. (2016). It takes two to tango : When two transcritptionnal mechanisms meet. Riboclub 2016. (Article publié).
  • Chauvier A*, St-Pierre P*, Nadon JF*, Perez Gonzalez D-C, Penedo-Esteiro J-C, Lafontaine D. (2016). RNA Folding Dynamics in Transcription Elongation Complexes Monitored at the Single-Molecule Level. Riboclub 2016. (Article publié).
  • Nadon JF*, Gonzalez DC, St-Pierre P*, Chauvier A*, Penedo JC, Lafontaine DA. (2016). The Need for Speed. 5th Zing Nucleic Acids Conference 2016 ****Best poster award****. (Article publié).
  • Nadon J*, Pérez-González C, St-Pierre P*, Chauvier A*, Penedo JC, Lafontaine DA. (2016). The need for speed: regulation of the thiC riboswitch using PIFE assays. Riboclub 2016. (Article publié).
  • Geffroy M*, Masse E, Lafontaine DA. (2016). Translation of a leader peptide is required for mgtA expression in Escherichia coli. Riboclub 2016 ***4th place poster competition, 2nd place voted by the public***. (Article publié).
  • Chauvier A*, Picard-Jean F, Dubé A*, Berger-Dancause JC*, Naghbi R, Perreault J, Lafontaine DA. (2015). Folding and regulation mechanism of the thiC riboswitch. Regulating with RNA in Bacteria and Archaea ***2nd place poster competition***. (Article publié).
  • Chauvier A*, Picard-Jean F, Dubé A*, Berger-Dancause JC*, Naghbi R, Perreault J, Lafontaine DA. (2015). Folding and regulation mechanism of the thiC riboswitch. RiboClub 2015 ***2nd place poster competition***. (Article publié).
  • Boudreault J*, Pérez González DC, Penedo JC, Lafontaine DA. (2015). Folding of the SAM-I riboswitch: A tale with a twist. RiboClub 2015. (Article publié).
  • Hérault E*, Massé E, Lafontaine DA. (2015). Regulation by Riboswitches, more than just a “cis history”. RiboClub 2015. (Article publié).
  • Geffroy M*, Singh N, Wade J, Massé E, Lafontaine DA. (2015). The mgtA riboswitch in Escherichia coli. Regulating with RNA in Bacteria and Archaea. (Article publié).
  • Geffroy M*, Singh N, Wade J, Massé E, Lafontaine DA. (2015). The mgtA riboswitch in Escherichia coli. RiboClub 2015. (Article publié).
  • Michaud A*, Smith-Peter E*, Lafontaine DA, Fortier LC. (2015). The riboswitch-controlled GMP synthase GuaA is important for survival and virulence of Clostridium difficile in vitro and in a mouse model of infection. RiboClub 2015. (Article publié).
  • Boudreault J*, Heppell B*, Dussault AM*, Lafontaine DA. (2014). Characterization of the folding pathway of the SAM-I riboswitch. Riboclub 2014. (Article publié).
  • Chauvier A*, Dubé A*, Naghbi R, Perreault J, Lafontaine DA. (2014). Folding and regulation mechanism of the thiC riboswitch. Riboclub 2014. (Article publié).
  • Chauvier A*, Lamontagne AM, Naghbi R, Perreault J, Lafontaine DA. (2014). Folding and regulation mechanism of the thiC riboswitch. RNA 2014. (Article publié).
  • Geffroy M*, Lafontaine DA. (2014). Nouveau mécanisme de régulation impliquant le contrôle d’un riborégulateur par la traduction d’un peptide leader. Conférences étudiantes de l'axe Inflammation et Douleur. (Article publié).
  • Bastet L*, Lamontagne AM, Massé E, Lafontaine DA. (2014). Riboswitch and small RNA-induced translation inhibition lead to mRNA degradation using different mechanisms. RNA 2014. (Article publié).
  • Geffroy M*, Lafontaine DA. (2014). The regulation mechanism of the E. coli mgtA riboswitch. Riboclub 2014. (Article publié).
  • Lussier A*, Bastet L*, Lafontaine DA. (2014). What do you need to switch with your B12 riboswitch?. Riboclub 2014. (Article publié).
  • Lamontagne AM, Archambault MA, Le-Roux A, Yan LH, Boyapelly K, Lalonde-Seguin D, Marseault E, Fortier LC, Lafontaine DA. (2013). Clostridium difficileNew antibiotic class targeting the guanine riboswitch in. Riboclub 2013. (Article publié).
  • Boudreault J*, Heppell B*, Dussault AM*, Eschbach SH*, Lafontaine DA. (2013). Identification of essential nucleotides in the SAM-I riboswitch folding pathway. Riboclub 2013. (Article publié).
  • Bastet L*, Caron MP*, Lussier A*, Simoneau-Roy M*, Massé E., Lafontaine DA. (2013). Riboswitch control of translation and mRNA decay. Regulating with RNA in bacteria. (Article publié).
  • Lussier A*, Chauvier A*, Lafontaine DA. (2013). Study of translational control of Escherichia coli riboswitches. Riboclub 2013. (Article publié).
  • Geffroy M*, Lafontaine DA. (2013). The regulation mechanism of the E. coli mgtA riboswitch. Riboclub 2013. (Article publié).
  • Geffroy M*, Bastet L*, Lafontaine DA. (2013). The translationally-acting E. coli AdoCbl riboswitch triggers mRNA decay through translation inhibition. Regulating with RNA in bacteria. (Article publié).
  • Bastet L*, Caron MP*, Massé E, Lafontaine DA. (2013). When the small RNA OmrB meet the CoB12 riboswitch in Escherichia coli. RiboClub 2013 ***2nd place poster prize***. (Article publié).
  • Chauvier A*, Dubé A*, Lafontaine D. (2013). thiC Deciphering the molecular mechanism of the riboswitch. Riboclub 2013. (Article publié).
  • Smith-Peter E*, Lamontagne AM, Lafontaine DA. (2012). Analysis of dynamic ligand capturing and rational design of antibiotics targeting riboswitches. RiboClub 2012. (Article publié).
  • Lamontagne AM, Mulhbacher J*, Lafontaine DA. (2012). Characterization of guanine riboswitches for the development of selective analog inhibitors. RNA meeting 2012. (Article publié).
  • St-Pierre P*, Penedo JC, Lafontaine DA. (2012). Influence of ligand binding on the loop-loop interaction of the adenine riboswitch aptamer. RNA 2012. (Article publié).
  • St-Pierre P*, Penedo JC*, Lafontaine DA. (2012). Influence of the ligand binding on the loop-loop interaction of the adenine binding riboswitch aptamer. RiboClub 2012. (Article publié).
  • Comeau MA*, Lafontaine DA, Abou Elela S. (2012). Product-based regulation of RNase III processivity. Riboclub 2012. (Article publié).
  • Bastet L*, Lussier A*, Dubé A*, Lafontaine DA. (2012). The butterfly effect of translationally-acting riboswitches. RNA 2012. (Article publié).
  • Simoneau-Roy M*, Dubé A*, Caron MP*, Massé E, Lafontaine DA. (2012). The thiB riboswitch of Escherichia coli might be a trans-acting riboswitch. RiboClub 2012. (Article publié).
  • Eschbach E*, Dussault AM*, Heppell B*, Lafontaine DA. (2012). Twisting or not twisting the SAM-Iriboswitches. RiboClub 2012. (Article publié).
  • Bastet L*, Lussier A*, Dubé A*, Lafontaine DA. (2012). vsDangerous liaisons: The CoB12 riboswitch the small RNA OmrB. Riboclub 2012. (Article publié).
  • Smith-Peter E*, Blouin S*, Lafontaine DA. (2011). Caractérisation du site de liaison du riborégulateur lysine. ACFAS 2011. (Article publié).
  • Smith-Peter E*, Blouin S*, Lafontaine DA. (2011). Caractérisation du site de liaison du riborégulateurlysine. Journée Phare 2011. (Article publié).
  • Eschbach S*, Dussault AM*, Heppell B*, Lafontaine DA. (2011). Comparison between a four-way junctionand a three-way junction SAM-I riboswitch. RiboClub 2011. (Article publié).
  • Dubé A*, Simoneau-Roy M*, Caron MP*, Massé E, Lafontaine DA. (2011). Evidences toward different TPP riboswitch regulation mechanisms in Escherichia coli. Regulating with RNA in Bacteria. (Article publié).
  • *Comeau MA, Abou Elela S, Lafontaine DA. (2011). Regulation of RNase III mediated RNA metabolism. RiboClub 2011. (Article publié).
  • Simoneau-Roy M*, Caron MP*, Massé E, Lafontaine DA. (2011). SroA: une riborégulation agissant en trans ?. ACFAS 2011. (Article publié).
  • Bastet *L, Dubé A*, Lafontaine DA. (2011). Studies of the Escherichia coli AdoCbl riboswitch regulation mechanisms. Regulating with RNA in Bacteria. (Article publié).
  • Dubé A*, Dussault AM*, Simoneau-Roy S*, Caron MP*, Massé E, Lafontaine DA. (2011). TPP riboswitches exhibit fundamental mechanistic differences in E coli. RiboClub 2011. (Article publié).
  • Bastet L*, Dubé A*, Lafontaine DA. (2011). The AdoCbl riboswitch: From the switch to the regulation mechanism. RiboClub 2011. (Article publié).
  • Simoneau-Roy M*, Caron MP*, Dubé A*, Massé E, Lafontaine DA ***2nd place for best poster presentation. (2011). The thiB riboswitch of Escherichia coli: A trans-acting riboswitch?. RiboClub 2011. (Article publié).
  • Blouin S*, Chinnappan R*, Penedo JC, Lafontaine DA. (2010). Folding of the lysine riboswitch:importance of peripheral elements for transcription regulation. RiboClub 2010. (Article publié).
  • Bastet L*, Lafontaine DA*. (2010). In vivo characterization of the Escherichia coli translationally-actingAdoCbl riboswitch. RiboClub 2010. (Article publié).
  • Dubé A, Caron MP*, Simoneau-Roy M*, Beaulne S*, Massé E, Lafontaine DA. (2010). In vivo regulation of TPP riboswitches in Escherichia coli. RiboClub 2010. (Article publié).
  • Caron MP*, Massé E, Lafontaine DA. (2010). In vivo study of lysine riboswitch mechanism in Escherichia coli. RNA 2010. (Article publié).
  • Caron MP*, Massé E, Lafontaine DA. (2010). In vivo study of the lysine riboswitch mechanism in Escherichia coli. RiboClub 2010. (Article publié).
  • Comeau MA*, Bisaillon M*, Abou Elela S, Lafontaine DA. (2010). Product-determined turnover rate of dsRNA processing. Riboclub 2010. (Article publié).
  • St-Pierre P*, Lemay JF*, Penedo JC, Lafontaine DA. (2010). Single molecule folding of a purine bindingRNA aptamer. RIboClub 2010. (Article publié).
  • Caron MP*, Desnoyers G*, Massé E, Lafontaine DA. (2009). In vivo study of lysine riboswitch in E. coli. Canadian Society for Microbiology (CSM). (Article publié).
  • Delfosse V, Bouchard P, Bonneau E, Dagenais P, Lemay JF*, Lafontaine DA, Legault P. (2009). Purine riboswitch structure: a unique evolutionnary-excluded structural mimicry. Annual GRASP Symposium. (Article publié).

Propriétés intellectuelles

Brevets

  • (2015). Guanine riboswitch binding compounds and their use as antibiotics. US 20150150890 A1. Canada. (Délivré).
  • (2015). Guanine riboswitch binding compounds and their use as antibiotics. EP 2440533 B1. Canada. (Délivré).
  • (2013). Guanine riboswitch binding compounds and their use as antibiotics. EP 2440533 A4. Canada. (Délivré).
  • (2012). Guanine riboswitch binding compounds and their use as antibiotics. EP 2440533 A1. Canada. (Délivré).
  • (2012). Guanine riboswitch binding compounds and their use as antibiotics. US 20120122819 A1. Canada. (Délivré).
  • (2010). Guanine riboswitch binding compounds and their use as antibiotics. WO2010/142027 A1. Canada. (Délivré).
  • (2010). Guanine riboswitch binding compounds and their use as antibiotics. CA 2778931 A1. Canada. (Délivré).

Autres contributions

Gestion d'évènements

  • Co-organizer. (2019). Riboclub 2019. (Conférence).
  • Co-organizer. (2018). Riboclub 2018. (Conférence).
  • Co-organizer. (2017). Riboclub 2017. (Conférence).
  • Co-organizer. (2016). Riboclub 2016. (Conférence).
  • Co-organizer. (2015). RiboClub 2015. (Conférence).
  • Co-organizer. (2014). RiboClub 2014. (Conférence).
  • Co-organizer. (2013). Riboclub 2013. (Conférence).
  • Co-organizer. (2012). RiboClub 2012. (Conférence).
  • Co-organizer. (2011). RiboClub 2011. (Conférence).
  • Co-organizer. (2011). Colloque ARN (ACFAS). (Atelier).

Activités de collaboration internationale

  • Reasearch advisor. (2019-2021). Norvège.
  • Research collaborator. (2015). États-Unis.
  • Research collaborator. (2006). Royaume-Uni.

Présentations

  • (2019). Real-time imaging of riboswitch transcription elongation. Dundee Nucleic Acids Conference. Dundee, Royaume-Uni.
  • (2018). Metabolite sensing in the TPP riboswitch is modulated during transcription elongation. BBB and CCBIO seminars. Bergen, Norvège.
  • (2017). L'importance de prendre une pause (moléculaire) pour bien faire les choses. Institut de Pharmacologie de Sherbrooke. Sherbrooke, Canada.
  • (2016). Real-time imaging of riboswitch regulatory mechanisms. Zing conference, 5th Nucleic Acid Meeting. Florida, États-Unis.
  • (2015). Study of riboswitch regulation mechanisms modulating mRNA levels. Regulating with RNA in Bacteria and Archaea Conference (Zing Conference). Mexico, Mexique.
  • (2015). Study of riboswitch regulation mechanisms modulating mRNA levels. RiboWest 2015. Edmonton, Canada.
  • (2014). Discontinuous transcription elongation dictates riboswitch sensing and regulation mechanisms. RiboClub 2014. Magog, Canada.
  • (2014). Dual-acting riboswitch control of translation initiation and mRNA decay. Invited by M Bayfield, Dept of Biology, University of York. Toronto, Canada.
  • (2014). Dual-acting riboswitch control of translation initiation and mRNA decay. Canadian Society for Chemistry. Vancouver, Canada.
  • (2014). Le secret du riboswitch. Les midis scientifiques du Cegep de Sherbrooke. Sherbrooke, Canada.
  • (2013). Dual-acting riboswitch control of translation initiation and mRNA decay. Invited by N Polacek, Dept. of Chemistry and Biochemistry, University of Bern. Bern, Suisse.
  • (2013). Dual-acting riboswitch control of translation initiation and mRNA decay. Invited by E Ennifar, Dept. Biologie structurale, Universite de Strasbourg. Strasbourg, France.
  • (2013). Molecular insights into the ligand-controlled organization of SAM-sensing riboswitches. Invited by J Wohnert, Dept. RNA-Strukturbiologie, Goethe-University. Frankfurt, Allemagne.
  • (2013). Novel riboswitch ligand analogs as selective inhibitors of metabolic pathways. Antibiotic Research Symposium. Bonn, Allemagne.
  • (2013). Riborégulateurs & applications cliniques. Journée scientifique des étudiants du CRHDQ (Centre de Recherche de l’Hôtel-Dieu de Québec). Québec, Canada.
  • (2013). Riborégulateurs & applications cliniques. 5ième Colloque de l’Association Collégiale de Biotechnologies. Sherbrooke, Canada.
  • (2013). Riboswitches & clinical applications. General Microbiology Spring Conference. Manchester, Royaume-Uni.
  • (2013). Riboswitches as novel antibacterial targets. Invited by biotech companies Néomed et Amorchem. Montréal, Canada.
  • (2013). Riboswitches as targets for novel classes of antibiotic. Canadian Society of Microbiologists. Ottawa, Canada.
  • (2013). Riboswitches as targets for novel classes of antibiotic. Canadian Society for Chemistry. Québec, Canada.
  • (2012). Riborégulateurs & applications cliniques. Symposium TEDx de l'Université de Sherbrooke. Sherbrooke, Canada.
  • (2012). Riboswitches & clinical applications. International Symposium on Staphylococci and Staphylococcal Infections. Lyon, France.
  • (2011). Controle du niveau de l'ARN messager du riborégulateur lysine. Biologie et Pharmacologie Structurale (ACFAS). Sherbrooke, Canada.
  • (2011). Implication du dégradosome dans un nouveau mécanisme de régulation génétique du riboswitch lysine de Escherichia coli. Axe d'infectiologie, Centre de recherches cliniques Université de Sherbrooke. Sherbrooke, Canada.
  • (2011). In vivo characterization of the lysine riboswitch. Invited by S Zimmerly, Dept. Biological Sciences, University of Calgary. Calgary, Canada.
  • (2011). Le côté obscur des riborégulateurs: quand un mécanisme de régulation en cache un autre. ACFAS. Sherbrooke, Canada.
  • (2011). Les mystères du riborégulateur adénosylcobalamine. Axe d'infectiologie, Centre de recherches cliniques Université de Sherbrooke. Sherbrooke, Canada.
  • (2011). New insights into riboswitch regulatory mechanisms. Eastern Riboclub. Sherbrooke, Canada.
  • (2011). Novel Riboswitch Ligand Analogs as Selective Inhibitors of Guanine-Related Metabolic Pathways. Technological Advances in RNA Therapy. Montréal, Canada.
  • (2011). Riborégulateurs & applications cliniques. Journée Phare de l'Institut de Pharmacologie de Sherbrooke. Magog, Canada.
  • (2011). Riboswitches & clinical applications. Invited by L Ferguson, Boehringer Ingelheim (Québec). Laval, Canada.
  • (2011). Single-molecule investigations of the folding rates of the pbuE adenine riboswitch. Eastern Riboclub. Orford, Canada.
  • Bastet L, Dubé A, Lafontaine DA. (2011). The dark side of the B12 riboswitch. Eastern RiboClub. Sherbrooke, Canada.
  • (2010). Folding and activity of the SAM riboswitch. Alumnus of the Year au RiboClub 2010. Magog, Canada.
  • (2010). Les riborégulateurs / Riboswitches. Invited at the Institut de Pharmacologie de Sherbrooke. Sherbrooke, Canada.
  • Comeau MA, Wieden HJ, Kothe U, Bisaillon M, Abou Elela S, Lafontaine DA. (2010). Product- Determined turnover rate of dsRNA processing. 6th annual meeting of the Ribowest. Lethbridge, Canada.
  • (2010). The regulatory nature of riboswitches determines the ligand binding mechanism. Eastern Riboclub. Orford, Canada.
  • (2010). Étude in vivo de mécanisme de régulation des riborégulateurs. Axe d'infectiologie, Centre de recherches cliniques Université de Sherbrooke. Sherbrooke, Canada.
  • Delfosse V, Bouchard P, Bonneau E, Dagenais P, Lemay JF, Lafontaine DA, Legault P. (2009). Etudes structurales du riboswich A adénine de Bacillus subtilis. Groupe Thématique de Biologie. Paris, France.
  • (2009). Folding and activity of the S-adenosyl methionine riboswitch. Invited by N Polacek, Medical University Division of Genomics and RNomics, Innsbruck Biocenter. Innsbruck, Autriche.
  • (2009). Folding and activity of the S-adenosyl methionine riboswitch. RNA Ligand Interactions. Frankfurt, Allemagne.
  • (2009). Folding and activity of the S-adenosyl methionine riboswitch. Invited by C Penedo, Dept. Physics and Astronomy, University of St-Andrews. St-Andrews, Royaume-Uni.
  • (2009). Folding and activity of the S-adenosyl methionine riboswitch. Invited by D Lilley, Dept. Biochemistry, The University of Dundee. Dundee, Royaume-Uni.