Boissonneault, Guylain

Professeur, Faculté de médecine et des sciences de la santé
FMSS Dép. de biochimie et de génomique fonction.

Coordonnées

Courriel


819-821-8000, poste 75443


Site Web

Diplômes

(1993) Post-doctorate (Molecular and Cell Biology). University of California, San Francisco.

(1989) Doctorate (Molecular and Cell Biology). Université Laval.

(1984) Bachelor's (Medical Biology). Université du Québec à Trois-Rivières.

Présentation

Sujets de recherche

Cell, Genomic Damage and Repair, Reproductive Biology and Dysfunctions.

Disciplines de recherche

Biology and Related Sciences, Molecular Biology.

Mots-clés

Cell culture, Chromatin, Chromosomal proteins, DNA repair, DNA topology, Expression systems, Gel electrophoresis, Immmunocytochemistry, Next generation sequencing, Spermatogenesis.

Intérêts de recherche

Our research interest focusses on the molecular events surrounding the chromatin remodeling in spermatids. The process involves the formation of transient DNA strand breaks. As this could represent an important source of genetic instability, will are investigating their distribution and the mechanisms by which they are created and repaired.

Centre de recherche

Centre de recherche du CHUS

Langues parlées et écrites

Anglais, Français

Financement

Grant. (Awarded). Principal Investigator. Genetic instability of the haploid male germ cells. Canadian Institutes of Health Research (CIHR). Project Grant. 669375 $ (2018-2023).

Grant. (Awarded). Principal Applicant. Nuclear activity of carnitine acetyltransferase. Natural Sciences and Engineering Research Council of Canada (NSERC). Discovery Grant. 145000 $ (2018-2023).

Grant. (Awarded). Co-applicant. Targeted DNA methylation analysis of the male germline:screening for environmental influence. Fonds Québécois de la Recherche sur la Nature et les Technologies (FQRNT). Subvention d'équipe. 205740 $ (2018-2021).

Grant. (Awarded). Principal Applicant. Development of a clinical assay to monitor the sperm genome integrity (SPRING test).. Ministère de l'Économie et de l'Innovation. Progr. de Soutien à la Valorisation et au Transfert. 218229 $ (2019-2020).

Grant. (Awarded). Principal Applicant. Genetic instability of the haploid male germ cells (Bridge funding). Canadian Institutes of Health Research (CIHR). Project Grant. 100000 $ (2018-2019).

Grant. (Awarded). Principal Applicant. DNA double-strand breaks assay for sperm genetic integrity. Aligo Innovation. Progr. soutien à la valorisation et au transfert. 20000 $ (2017-2018).

Publications

Articles de revue

  • Cavé, T., Desmarais, R., Lacombe-Burgoyne, C., Boissonneault, G. (2019). Genetic instability and chromatin remodeling in spermatids. Genes (Basel), 10(1), 1-10. (Published).
  • Cavé, T.*, Brazeau, M.-A.*, Grégoire, M.-C.*, Boissonneault, G. (2018). Post-meiotic DNA double-strand breaks are conserved in fission yeast. International Journal of Biochemistry and Cell Biology, 98(N/A), 24-28. (Published).
  • Julien Massonneau*, Camille Ouelette, Fabrice Lucien, Claire M. Dubois, Jessica Tyler, Guylain Boissonneault. (2018). Suboptimal pHs alter DNA damage response to induced double-stranded breaks. FEBS Open Bio, 8(3), 416-425. (Published).
  • Grégoire, M.-C.*, Leduc, F., Morin, M., Cavé, T.* Arguin, M., Richter, M., Jacques, P.-E., Boissonneault, G. (2018). The DNA double-strand «breakome» of mouse spermatids. Cell & Molecular Life Sciences https://doi.org/10.1007/s00018-018-2769-0, N/A, N/A. (Published).
  • Simard, O.*, Niavarani, SR*, Gaudreault, V*, Boissonneault, G. (2017). Torsional stress promotes trinucleotidic expansion in spermatids. Mutation Research, 800-820, 1-7. (Published).
  • Marie-Chantal Grégoire*, Julien Massonneau*, Frédéric Leduc*, Mélina Arguin*, Marc-André Brazeau* and Guylain Boissonneault. (2016). Quantification and genome-wide mapping of DNA double-stranded breaks. DNA Repair (Amst), 48, 63-68. (Published).
  • François Peyresaubes, Annie D’Amours, Frédéric Leduc*, Marie-Chantal Grégoire*, Guylain Boissonneault and Antonio Conconi. (2015). Immuno-capture of UVDE generated 3'-OH ends at UV photoproducts. DNA repair, 36, 156-161. (Published).
  • Simard O*, Leduc F*, Acteau G*, Arguin M*, Grégoire MC*, Brazeau MA*, Marois I, Richter MV, Boissonneault G. (2015). Step-specific sorting of mouse spermatids by flow cytometry. Journal of Visualized Experiments, 106, e53379. (Published).
  • Olivier Simard*, Marie-Chantal Grégoire*, Mélina Arguin, Marc-André Brazeau*, Frédéric Leduc*, Isabelle Marois, Martin V. Richter, Guylain Boissonneault. (2014). Instability of trinucleotidic repeats during chromatin remodeling in spermatids. Human Mutation, 35(11), 1280-1284. (Published).
  • Anne Gouraud*, Marc-André Brazeau*, Marie-Chantal Grégoire*, Olivier Simard*, Julien Massonneau*, Mélina Arguin and Guylain Boissonneault. (2013). Breaking news from spermatids. Basic and Clinical Andrology, 23(11), 1-5. (Published).
  • Grégoire MC*, Massonneau J*, Simard O*, Gouraud A*, Brazeau MA*, Arguin M, Leduc F*, Boissonneault G. (2013). Male-driven de novo mutations in haploid germ cells. Molecular Human Reproduction, 19(8), 495-499. (Published).

Chapitres de livre

  • Cavé, T.*, Simard, O.*, Boissonneault, G. (2017). Defective DNA repair in spermiogenesis. Ashock Agarwal and Armand Zini A clinician’s guide to sperm DNA and chromatin damage (Chapt 13). Switzerland : Springer International. (Published).

Propriétés intellectuelles

Patents

  • DNA Double-stranded breaks assay for sperm genetic integrity. 35-9-1 (Internal Reference). Canada. (Pending).

Autres contributions

Activités de collaboration internationale

  • Scientific collaborator. (2014-2020). United States. Influence of subphysiological pH on genetic instability Collaboration with Dr Jessica Tyler (Weill Cornell Medical College, NY) Ungoing collaboration.

Présentations

  • Simard, O., Grégoire, M.-C. (2016). More «breaking» news from spermatids. 41st Annual Meeting of the American Society of Andrology, New Orleans, LA April 1-5, 2016. New Orleans, United States.
  • Boissonneault G., Simard O., Grégoire M.C. (2014). Breaking news from spermatids. Chromothripsis, Clustered Mutation and Complex Chromosome Rearrangements. Cambridge, United States.
  • Gouraud A., Grégoire M.-C.,. (2014). Nuclear acetyltransferase (CRAT) as a new evidence of mitochondrial – nuclear crosstalk mechanism involved in the haploid male germ cell differentiation. 2nd Canadian Conference on Epigenetics. London, Canada.
  • Simard, O, Grégoire M-C, Brazeau M-A, Leduc F. (2013). Chromatin remodeling in spermatids and paternal anticipation of trinucleotidic diseases. The 59th CFAS Annual Meeting. Victoria, Canada.
  • Grégoire, M.-C., Leduc F, Massonneau J, Simard O, Hmamouchi R, Marois I, Arguin M, Richter M, Rodrigue S, Jacques P-E,. (2013). Chromatin remodelling: a potential threat to sperm genetic integrity. 46th annual meeting of the society for the study of reproduction. Montreal, Canada.