Steve Jean

Prix et distinctions

• (2023) FRQS, Chercheur Boursier Junior 2. Fonds de recherche du Québec - Santé (FRQS). (Prize / Award).
• (2020) CIHR New Investigator Salary Award : 2014-2015. Canadian Institutes of Health Research. (Prize / Award).
• (2019) FRQS, Chercheur Boursier Junior 1 - Declined. Fonds de recherche du Québec - Santé (FRQS). (Prize / Award).
• (2018) Dean's list for exceptional professoral performance. Université de Sherbrooke. (Prize / Award).
• (2017) Dean's list for exceptional professoral performance. Université de Sherbrooke. (Prize / Award).
• (2015) American Heart Association Post-doctoral Fellowship - Declined. American Heart Association. (Prize / Award).
• (2015) Scholarship for the Next Generation of Scientists. Cancer Research Society. (Prize / Award).
• (2012) FRQS Postdoctoral fellowship. Fonds de recherche du Québec - Santé (FRQS). (Prize / Award).
• (2009) Honors Board for Ph.D. thesis. Université Laval. (Distinction).
• Best Oral Presentation. Journée de la Faculté de médecine. (Prize / Award).
• Best Poster Presentation. Congrès Signalisation Québec. (Prize / Award).
• Best Poster Presentation. International Meeting on Cytoskeleton Dynamics. (Prize / Award).
• Corresponding author publication recommended by F1000. Faculty of 1000. (Citation).
• Highest academic average of microbiology promotion 2001. Université Laval. (Distinction).
• Poster Award (First Jury Prize) Lab Oratoire. Laval University Graduate Student Association (AELIES). (Prize / Award).
• Poster Award (Public Prize) Lab Oratoire. Laval University Graduate Student Association (AELIES). (Prize / Award).
• Poster Presentation Award. Cancer Research Center Student's Scientific Day. (Prize / Award).
• Travel Award. Cold Spring Harbor Laboratory. (Prize / Award).
• Travel Award. CIHR National Poster Competition. (Prize / Award).
• Travel Award. 11th International Xenopus Meeting. (Prize / Award).
• Travel Award. Université Laval. (Prize / Award).
• Travel Award. Université Laval. (Prize / Award).
• Travel Award. Université Laval. (Prize / Award).
• Travel Award. 10th International Xenopus meeting. (Prize / Award).
• Travel Award. Gordon Research Conference. (Prize / Award).

Financement

• Grant. (Under Review). Principal Applicant. Endosomal actin regulation by a RAB GTPase. Canadian Institutes of Health Research (CIHR). Project grants. 1 050 000 $. (2020-2025)
• Grant. (Awarded). Principal Applicant. In vivo roles of autophagy in gut homeostasis and impact on IBD. Canadian Institutes of Health Research (CIHR). 2020 Project Grant Competition. 895 000 $. (2020-2025)
• Research Chair. (Awarded). Principal Investigator. Endosomal regulation of autophagy in intestinal diseases. Centre de Recherche Médicale de l'UdeS. Junior Faculty Research Chair. 240 000 $. (2018-2023)
• Grant. (Awarded). Principal Investigator. Coordination of phosphoinositides and RAB GTPases in membrane trafficking events. Natural Sciences and Engineering Research Council of Canada (NSERC). Discovery Grants. 216 000 $. (2016-2022)
• Grant. (Completed). Principal Applicant. Signalling functions in cancer for a RAB GTPase. Cancer Research Society (The). Operating Grants. 120 000 $. (2017-2019)
• Grant. (Completed). Co-investigator. Harnessing CRISPR/Cas9 technology for fundamental and translational cancer studies. Centre Recherche en Inflammation et Oncologie Digestive de l'Université de Sherbrooke (CRIODUS). Structuring grant. 18 000 $. (2018-2019)
• Grant. (Completed). Principal Applicant. Développement d’un nouveau modèle de cancer colorectal chez la drosophile: étude de l’efficacité thérapeutique de l’autophagie et effet du statut de p53 dans ce modèle.. CRIODUS. Structuring grant. 15 000 $. (2016-2017)
• Grant. (Completed). Principal Investigator. In vivo studies of autophagy: from the model organism to human organoids. Canada Foundation for Innovation (CFI). John R Evans Leaders Fund. 952 000 $. (2017)

Publications

Articles de revue

• Lacasse, V., Beaudoin S., Jean S. and Leyton JV. (2020). A novel and generalizable proteomic method reveals classical NLS-tagging of trastuzumab-emtansine contravenes classical nuclear transport in a model of HER2-positive breast cancer. Molecular Therapy Methods & Clinical Development - -. (In Press).
• *Nassari., S, *Del Olmo, T., Jean S. (2020). Rabs in Signaling and Embryonic Development. International Journal of Molecular Sciences 21 (3), 1064. (Published).
• *Del Olmo T, *Lauzier A, *Normandin C, *Larcher R, *Lecours M, *Jean D, *Lessard L, Steinberg F, Boisvert FM and Jean S. (2019). APEX2-mediated RAB proximity labeling identifies a RAB21 role in clathrin-independent cargo sorting. EMBO Reports 20 (2), 1-21. (Published).
• *Lauzier A, *Normandeau-Guimond J, Vaillancourt-Lavigueur V, Boivin V, Charbonneau M, Rivard N, Scott MS, Dubois CM, Jean S. (2019). Colorectal cancer cells respond differentially to autophagy inhibition in vivo. Scientific Reports 9 11316. (Published).
• Ugrankar R, Bowerman J, Hariri H, Chandra M, Chen K, *Bossanyi MF, Datta S, Rogers S, Eckert KM, Vale G., Victoria A, Fresquez J, McDonald JG, Jean S, Collins BM and Henne MW. (2019). Drosophila Snazarus Regulates a Lipid Droplet Population at Plasma Membrane-Droplet Contacts in Adipocytes. Developmental Cell 50 557-572. (Published).
• Brunet, MA., Jacques JF., *Nassari S., Tyzack GE, McGoldrick P., Zinman L., Jean S., Robertson J., Patani R., Roucou X. (2019). FUS gene is dual-coding with both proteins united in molecular hallmarks of amyotrophic lateral sclerosis. BioRxiv - -. (Submitted).
• Binda C, Génier S, Picard S, Degrandmaison J, Fréchette L, Jean S, Marsault E, Parent JL. (2019). L-type prostaglandin D synthase regulates the trafficking of the PGD2 DP1 receptor through an interaction with Rab4. Journal of Biological Chemistry - -. (Published).
• Delos Santos K, Yergeay C, Kim M, Jean S, Moon NS. (2019). Pleiotropic Role of Drosophila Phosphoribosyl Pyrophosphate Synthetase in Autophagy and Lysosome Homeostasis. PLOS Genetics - -. (Published).
• *Del Olmo T, *Lacarrière-Keïta C, *Normandin C, *Jean D, Boisvert FM, Jean S. (2019). RAB21 interacts with TMED10 and modulates its localization and abundance. Biology Open - -. (Published).
• Paquette M, Beaudoin S, Tremblay MA, Jean S, Lopez A, Lecomte R, Guérin B, Bentourkia M, Sabbagh R, Leyton J. (2018). NLS-cholic acid conjugation to IL-5R-specific antibody improves cellular accumulation and in vivo tumor-targeting properties in a bladder cancer model. Bioconjugate Chemistry 18 1352-1363. (Published).
• Fujita N, Haung W, Lin TH, Groulx JF, Jean S, Nguyen J, Kuchitsu Y, Koyama-Honda I, Mizushima N, Fukuda M and Kiger AA. (2017). Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy. eLife 6 (e23367), -. (Published).
• Klionsky DJ et al. including Steve Jean. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy 12 (1), 1-222. (Published).
• Jean S and Kiger AA. (2016). Rab21 activity measurement using GST-fused APPL1. Bio-Protocol 6 (4), e1739. (Published).
• Jean S and Kiger AA. (2016). VAMP8-3xHA uptake assays in HeLa cells. Bio-Protocol 6 (4), e1738. (Published).
• Jean S, Cox S, Nassari S* and Kiger AA. (2015). Starvation-induced MTMR13 and RAB21 activity regulates VAMP8 trafficking to promote autophagosome-lysosome fusion. EMBO Reports 16 (3), 297-311. (Published).
• Jean S and Kiger AA. (2014). Classes of phosphoinositide 3-kinases at a glance. Journal of Cell Science 127 (5), 923-928. (Published).
• Jean S and Kiger AA. (2012). Coordination between RAB GTPase and phosphoinositide regulation and functions. Nature Reviews Molecular Cell Biology 13 (7), 463-470. (Published).
• Jean S, Cox S, Schmidt EJ, Robinson FL and Kiger AA. (2012). Sbf/MTMR13 coordinates PI(3)P and Rab21 regulation in endocytic control of cellular remodeling. Molecular Biology of the Cell 23 (14), 2723-2740. (Published).
• Jean S, Tremblay MG, Herdman C, Guillou F and Moss T. (2012). The endocytic adapter E-Syt2 recruits the p21 GTPase activated kinase PAK1 to mediate actin dynamics and FGF signalling. Biology Open 1 (8), 731-738. (Published).
• Michaela Velichkova, Joe Juan, Pavan Kadandale, Steve Jean, Inês Ribeiro, Vignesh Raman, Chris Stefan and Amy Kiger. (2010). Drosophila Mtm and class II PI3K coregulate a PI(3)P pool with cortical and endolysosomal functions. Journal of Cell Biology 190 (3), 407-425. (Published).
• Steve Jean, Alexander Mikryukov, MIchel G Tremblay, Jöelle Baril, François Guillou, Sabrina Bellenfant and Tom Moss. (2010). Extended-Synaptotagmin-2 Mediates FGF Receptor Endocytosis and ERK Activation In Vivo. Developmental Cell 19 (3), 426-39. (Published).
• Steve Jean and Tom Moss. (2008). A ubiquitin-conjugating enzyme, ube2d3.2, regulates xMLK2 and pronephros formation in Xenopus. Differentiation 76 (4), 431-441. (Published).
• Luc Poitras, Steve Jean, Nazrul Islam and Tom Moss. (2003). PAK interacts with NCK and MLK2 to regulate the activation of jun N-terminal kinase. FEBS Letters 543 (1-3), 129-135. (Published).
• Nicolas Bisson, Nazrul Islam, Luc Poitras, Steve Jean, Anne Bresnick, Tom Moss. (2003). The catalytic domain of xPAK1 is sufficient to induce myosin II dependent in vivo cell fragmentation independently of other apoptotic events. Developmental Biology 263 (2), 264-281. (Published).

Chapitres de livre

• *Lauzier A. and Jean S. (2018). Autophagic flux assessment in colorectal cancer cells. Jean-François Beaulieu. Methods in molecular biology - Colorectal cancer (167-175). United States : Springer. (Published).

Articles de conférence

• *Normandin C. and Jean S. A drosophila eye modifier screen identifies potential GTPase Activating Protein implicated in autophagy. Signalisation Québec 2018.
• *Normandin C. and Jean S. A drosophila eye modifier screen identifies potential GTPase Activating Protein implicated in autophagy. Autophagy in Stress, Development and Disease, Gordon Research Conference.
• *Normandeau-Guimond J. and Jean S. Analyse du flux autophagique de cellules cancéreuses colorectales. Signalisation Québec 2016.
• *Del Olmo T., *Jean D., Boisvert FM., Jean S. Definition of the Rab21 interactome through complementary quantitative mass spectrometry approaches. Société canadienne pour les Biosciences moléculaires 2017.
• *Normandin C. and Jean S. Drosophila A eye modifier screen identifies potential Rab21 GTPase Activating Proteins. Canadian National Proteomic Network.
• *Del Olmo T., *Lecours M., *Lauzier A., *Jean D., Boisvert FM., Jean S. Dynamic APEX2-mediated Rab proximity labeling identifies a Rab21 role in mTOR signaling. FASEB : Small GTPases in membrane processes: Trafficking, Autophagy and Disease.
• *Del Olmo T. *Lecours M., *Jean D. Boisvert FM., Jean S. Early endosomal Rab21 mediates mTOR kinase trafficking. Chronic Inflammation and Cancer 2019.
• *Normandin C. and Jean S. Identification et caractérisation de GAPs spécifiques à Rab21. Journée Phare 2016.
• *Normandin C. and Jean S. Identification et caractérisation de GAPs spécifiques à Rab21. Signalisation Québec 2016.
• *Normandin C. and Jean S. Identification et caractérisation de GAPs spécifiques à Rab21. Club de Recherche Clinique du Québec.
• *Bossanyi MF., Boucher MJ., Jean S. Implication de Snazarus et de Rab21 dans la formation de gouttelettes lipidiques. Club de Recherche Clinique du Québec.
• *Bossanyi MF., Boucher MJ., Jean S. Implication de Snazarus et de Rab21 dans la formation de gouttelettes lipidiques. Signalisation Québec 2018.
• *Del Olmo T., *Lauzier A., *Jean D., Boisvert FM., Jean S. Interactome de Rab21 généré par ligation de proximité identifie un nouveau rôle pour Rab21 avec le complexe WASH. Signalisation Québec 2018.
• *Del Olmo T. and Jean S. Interactome fonctionnel de Rab21 et son rôle dans la tumorigenèse. Signalisation Québec 2016.
• *Del Olmo T. and Jean S. Le protéome de Rab21 et son rôle dans le cancer. Journée Phare 2016.
• *Del Olmo T. and Jean S. Le protéome de Rab21 et son rôle dans le cancer. Club de Recherche Clinique du Québec.
• Jean S., Cox S., Kiger A. Rab21 and Sbf regulate autophagosome-lysosome fusion. CanFly XIII 2015.
• *Nassari S. and Jean S. Rab21 contributes to enterocytes survival and participates to intestinal renewal in a non-cell autonomous manner. 2019 European Drosophila Research Conference.
• *Nassari S. and Jean S. Rab21 in enterocytes participates to the maintenance of the intestinal epithelium. Chronic Inflammation and Cancer 2019.
• *Bossanyi M. and Jean S. Rab21 modulates Snazarus localization and impacts lipid droplets biogenesis. FASEB - The Lipid Droplets Conference.
• Jean S. Regulation of gut homeostasis by membrane trafficking proteins. Canadian Digestive Diseases Week (CDDW).
• *Nassari S. and Jean S. Rôle de Rab21 dans l’homéostasie intestinal chez la drosophile. Signalisation Québec 2018.
• *Lacarrière C. and Jean S. Rôle et régulation de la pseudophosphatase MTMR13/SBF dans l’homéostasie intestinale. Journée Phare.
• *Lacarrière C. and Jean S. Sbf and Rab21, two autophagy regulators involved in intestinal cell differentiation. Flying Through the Gut international seminar series.
• *Lacarrière C. and Jean S. Sbf and Rab21: two autophagy regulators involved in intestinal cell fate determination. Canadian Digestive Diseases Week (CDDW).
• *Lacarrière C. and Jean S. Sbf and Rab21: two autophagy regulators involved in intestinal cell fate determination. Chronic Inflammation and Cancer 2019.
• *Lacarrière C. and Jean S. Sbf and Rab21: two autophagy regulators involved in intestinal cell fate determination. XV Canadian Drosophila Conference.
• *Bossanyi MF., Ugrankar R., Henne MW., Jean S. Snazarus and modulates lipid droplet biogenesis. XV Canadian Drosophila Conference.
• Jean S., Cox S., Kiger A. Starvation-induced MTMR13 and Rab21 activity promotes VAMP8 autophagosome-lysosome fusion. 2014 Annual drosophila research conference.
• *Normandin C. and Jean S. TBC1D25 dans la régulation négative de Rab21, une protéine impliquée dans divers processus tumoraux. Club de Recherche Clinique du Québec.
• *Normandin C. and Jean S. TBC1D25 une GAP potentielle de Rab21. Journée Phare 2017.
• *Lauzier A. and Jean S. Targeting autophagy in colorectal cancer: a relevant strategy?. Autophagy in Stress, Development and Disease, Gordon Research Conference.
• *Normandin C. and Jean S. The GTPase activating protein TBC1D25 modulates multiple Rab21 functions. Chronic Inflammation and Cancer 2019.
• *Nassari S. and Jean S. The contribution of membrane trafficking in enterocytes survival. Flying Through the Gut seminar series.
• *Nassari S. and Jean S. The non-cell autonomous contribution of Rab21 in Drosophila gut renewal. 60th International Drosophila Conference.
• *Lauzier A. and Jean S. The sorting nexin Snazarus regulates autophagosome-lysosome fusion. 58th International Drosophila Conference.

Autres contributions

Présentations

• *Nassari S., Jean S. (2019). Endosomal and autophagic regulation of intestinal homeostasis. Chronic Inflammation, Immunity and Cancer. Orford, Canada
• Del Olmo T., Lauzier A., Jean S. (2018). APEX2-mediated proximity labelling of RAB GTPases identifies new trafficking roles for RAB21. Gordon Research Conference Lysosomes and Endocytosis. Andover, United States
• Jean S. (2018). APEX2-mediated proximity labelling of RAB GTPases identify a role for RAB21 in clathrin independent endocytosis. Signaling in Normal and Cancer Cells. Banff, Canada
• Jean S. (2018). Proteomic and Genetic Approaches to Decipher RAB functions. Hôtel-Dieu de Québec Seminar Series. Québec, Canada
• Jean S. (2018). Proteomic and Genetic Approaches to Decipher RAB functions. Sick Kids Seminar Series. Toronto, Canada
• Jean S. (2018). Proteomic and Genetic Approaches to understand RAB GTPases roles. Département de Biochimie – Université de Montréal Seminar Series. Montreal, Canada
• Jean S. (2018). Targeting autophagy in colorectal cancer cells: a relevant strategy?. Discovery on Target 2018 – Autophagy Workshop. Boston, United States
• Jean S. (2017). Endosomal regulation of autophagy. Canadian Drosophila Research Conference XIV. Banff, Canada
• Jean, S. (2017). Inhiber l’autophagie dans le cancer : un pensez-y-bien!. Cancer and inflammation research axis retreat. Canada
• Jean S. (2017). La génétique en recherche biomédicale. 18e Forum International Science et Société ACFAS. Sherbrooke, Canada
• Lauzier A, Jean S. (2017). The Sorting Nexin Snazarus regulates autophagosome-lysosome fusion. 58th International Drosophila Conference. San Diego, United States
• Jean S., Leung G. (2017). The fly and zebrafish as models to study GI tract disorders. Canadian Digestive Disease Week 2017. Banff, Canada
• Jean S. (2016). Endosomal regulation of autophagy. McGill University, Department of Biology. Guest speaker invitation for their seminar series. Montreal, Canada
• Jean S. (2016). Identification de nouveaux régulateurs du flux autophagique et rôles dans le cancer. Cancer research axis retreat. Canada
• Jean S. (2016). Regulation of gut homeostasis by membrane trafficking proteins. 2016 CDDW. Montréal, Canada
• Jean S. (2015). Nouvelles fonctions du système endosomal dans l'autophagie et le cancer colorectal. Centre de recherche du CHUS. Guest speaker invitation for seminar series. Sherbrooke, Canada
• Jean S., Cox S. and Kiger A. (2015). Rab21 and Sbf regulate autophagosome-lysosome fusion. CanFly XIII 2015. Montréal, Canada
• J. Groulx, S. Jean, N. Fujita, M. Velichkova, P. Kadandale, S. Cox, A.A. Kiger. (2015). Roles for Class II PI3K and Mtm in autophagy. American Society of Cell Biology 2015 Meeting. San Diego, United States
• Jean S., Cox S., Nassari S., Kiger A. (2014). Régulation endosomale de l’autophagie. Signalisation Québec 2014. Bécancour, Canada
• Jean S., Cox S., Kiger A. (2014). Starvation-induced MTMR13 and Rab21 activity promotes VAMP8 autophagosome-lysosome fusion. 9th EMBO/Workshop, Protein and lipid function in secretion and endocytosis. Goldegg am See, Austria
• Jean S., Cox S., Kiger A. (2014). Starvation-induced MTMR13 and Rab21 activity promotes VAMP8 autophagosome-lysosome fusion. 2014 Annual drosophila research conference. San Diego, United States
• Jean S., Cox S., Kiger A. (2013). Rab21 and Sbf are required for autophagic flux. Autophagy in cell stress, SDCSB symposium. San Diego, United States
• Jean S., Cox S., Kiger A. (2012). Coordination of PI(3)P and Rab21 in cell remodeling. Phosphoinositide Biology, Signaling and Cancer Symposium. Montréal, Canada
• Jean S., Cox S., Kiger A. (2012). Coordination of PI(3)P and Rab21 in cell remodeling and autophagy. Centre de Recherche en Cancérologie de l’Université Laval. Québec, Canada
• Jean S., Cox S., Kiger A. (2012). Coordination of PI(3)P and Rab21 in cell remodeling and autophagy. Département d’anatomie-biologie cellulaire Université Sherbrooke. Sherbrooke, Canada
• Jean S., Cox S., Kiger A. (2012). Coordination of PI(3)P and Rab21 in membrane trafficking. Gordon Research Conference. Andover, United States
• Jean S., Cox S., Kiger A. (2012). Coordination of PI(3)P and Rab21 in membrane trafficking. Gordon Research Seminar. Andover, United States
• Velichkova M., Kadandale P., Jean S., Cox S., Kiger A. (2012). Not all PI(3)P is created equal: Roles of class II PI3-kinase in metazoan autophagy. Gordon Research Conference. Ventura, United States
• Velichkova M., Kadandale P., Jean S., Kiger A. (2011). Class II PI3-Kinase and Regulators of Membrane Trafficking Coordinately Regulate Autophagy. Autophagy Keystone Symposia. Whistler, Canada
• Jean S., Ribeiro I., Velichkova M., Kadandale P., Kiger A. (2011). Coordinated PI3KC2/Mtm Phosphoinositide Regulation and Rab activity in Membrane Trafficking and Disease. Inositide Signaling in Pharmacology and Disease Keystone Symposia. Boulder, United States
• Jean S., Cox S., Kiger A. (2011). Coordination of phosphoinositide and Rab GTPase regulation and functions. Goethe Universität Frankfurt. Frankfurt, Germany
• Jean S, Cox S, Kiger A. (2011). Sbf, an MTM pseudophosphatase, coregulates PI(3)P homeostasis and Rab21 activity with essential requirements for hemocyte shape. International Drosophila Research conference. San Diego, United States
• Jean S., Cox S., Robinson F., Kiger A. (2011). Sbf/MTMR13 coordinates PI(3)P and Rab21 regulation in endocytic control of cellular remodeling. UCSD biology retreat. Lake Arrowhead, United States
• Jean S., Cox S., Robinson F., Kiger A. (2010). Sbf, an Mtm pseudophosphatase, more than meets the eye!. UCSD biology retreat. Lake Arrowhead, United States
• Jean S., Velichkova M., Juan J. and Kiger A. (2009). The myotubularin pseudophosphatase, Sbf, scaffolds a protein complex comprising Mtm, a catalytically active myotubularin phosphatase, and the class II PI3K, PI3K68D, to regulate PI(3)P dynamic. American Society for Cell Biology Meeting. San Diego, United States