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Xavier Roucou

Professeur, Faculté de médecine et des sciences de la santé
FMSS Département de biochimie et de génomique fonctionnelle

Présentation

Expériences académiques

  • Directeur baccalauréat en Biochimie de la santé. (2017-). Université de Sherbrooke. Sherbrooke, Quebec, Canada.
  • Professeur titulaire. (2004-). Université de Sherbrooke. Sherbrooke, Quebec, Canada.
  • Directeur de département. (2017-2025). Université de Sherbrooke. Sherbrooke, Quebec, Canada.

Publications

Articles

  • ElChoueiry, M., Sidhu, H., Lévesque, M., Lévesque, D., Jacques, J.-F., Sarrhini, O., Beaudoin, J.-F., Caron, M., Gaudette, B., Lecomte, R., Roucou, X., Boisvert, F.-M., Brosseau, J.-P. (2025). Development, validation, and preliminary phenotypic characterization of a Col6a3 knockout mouse model targeting exon 3. Animal Models and Experimental Medicine. DOI
  • Comtois, F., Jacques, J.-F., Métayer, L., Ouedraogo, W.Y.D.D., Ouangraoua, A., Denault, J.-B., Roucou, X. (2025). Noncanonical altPIDD1 protein: unveiling the true major translational output of the PIDD1 gene. Life Science Alliance. DOI
  • Garcia-del Rio, D.F., Derhourhi, M., Bonnefond, A., Leblanc, S., Guilloy, N., Roucou, X., Eyckerman, S., Gevaert, K., Salzet, M., Cardon, T. (2024). Deciphering the ghost proteome in ovarian cancer cells by deep proteogenomic characterization. Cell Death and Disease. DOI
  • Leblanc, S., Yala, F., Provencher, N., Lucier, J.-F., Levesque, M., Lapointe, X., Jacques, J.-F., Fournier, I., Salzet, M., Ouangraoua, A., Scott, M.S., Boisvert, F.-M., Brunet, M.A., Roucou, X. (2024). OpenProt 2.0 buildsã path to the functional c harãct erization ofãlt ernative proteins. Nucleic Acids Research. DOI
  • Kienzle, L., Bettinazzi, S., Choquette, T., Brunet, M., Khorami, H.H., Jacques, J.-F., Moreau, M., Roucou, X., Landry, C.R., Angers, A., Breton, S. (2023). A small protein coded within the mitochondrial canonical gene nd4 regulates mitochondrial bioenergetics. BMC Biology. DOI
  • Frion, J., Meller, A., Marbach, G., Lévesque, D., Roucou, X., Boisvert, F.-M. (2023). CRISPR/Cas9-mediated knockout of the ubiquitin variant UbKEKS reveals a role in regulating nucleolar structures and composition. Biology Open. DOI
  • Khatir, I., Brunet, M.A., Meller, A., Amiot, F., Patel, T., Lapointe, X., Avila Lopez, J., Guilloy, N., Castonguay, A., Husain, M.A., Germain, J.S., Boisvert, F.-M., Plourde, M., Roucou, X., Laurent, B. (2023). Decoupling of mRNA and Protein Expression in Aging Brains Reveals the Age-Dependent Adaptation of Specific Gene Subsets. Cells. DOI
  • Gagnon, M., Savard, M., Tran, T.M.H., Vincent, L., Moquin, A., Tremblay, P., Roucou, X., Dory, Y., Gobeil, F. (2023). Evaluation of Novel B1R/B2R Agonists Containing TRIOZAN™ Nanoparticles for Targeted Brain Delivery of Antibodies in a Mouse Model of Alzheimer Disease. Molecules. DOI
  • Çakır, U., Gabed, N., Brunet, M., Roucou, X., Kryvoruchko, I. (2023). Mosaic translation hypothesis: chimeric polypeptides produced via multiple ribosomal frameshifting as a basis for adaptability. FEBS Journal. DOI
  • Leblanc, S., Brunet, M.A., Jacques, J.-F., Lekehal, A.M., Duclos, A., Tremblay, A., Bruggeman-Gascon, A., Samandi, S., Brunelle, M., Cohen, A.A., Scott, M.S., Roucou, X. (2023). Newfound Coding Potential of Transcripts Unveils Missing Members of Human Protein Communities. Genomics Proteomics and Bioinformatics. DOI
  • Guilloy, N., Brunet, M.A., Leblanc, S., Jacques, J.-F., Hardy, M.-P., Ehx, G., Lanoix, J., Thibault, P., Perreault, C., Roucou, X. (2023). OpenCustomDB: Integration of Unannotated Open Reading Frames and Genetic Variants to Generate More Comprehensive Customized Protein Databases. Journal of Proteome Research. DOI
  • Manuel, J.M., Guilloy, N., Khatir, I., Roucou, X., Laurent, B. (2023). Re-evaluating the impact of alternative RNA splicing on proteomic diversity. Frontiers in Genetics. DOI
  • Brunet, M.A., Leblanc, S., Roucou, X. (2022). OpenVar: functional annotation of variants in non-canonical open reading frames. Cell and Bioscience. DOI
  • Mudge, J.M., Ruiz-Orera, J., Prensner, J.R., Brunet, M.A., Calvet, F., Jungreis, I., Gonzalez, J.M., Magrane, M., Martinez, T.F., Schulz, J.F., Yang, Y.T., Albà, M.M., Aspden, J.L., Baranov, P.V., Bazzini, A.A., Bruford, E., Martin, M.J., Calviello, L., Carvunis, A.-R., ... van Heesch, S. (2022). Standardized annotation of translated open reading frames. Nature Biotechnology. DOI
  • Mohaupt, P., Roucou, X., Delaby, C., Vialaret, J., Lehmann, S., Hirtz, C. (2022). The alternative proteome in neurobiology. Frontiers in Cellular Neuroscience. DOI
  • Brunet, M.A., Lucier, J.-F., Levesque, M., Leblanc, S., Jacques, J.-F., Al-Saedi, H.R.H., Guilloy, N., Grenier, F., Avino, M., Fournier, I., Salzet, M., Ouangraoua, A., Scott, M.S., Boisvert, F.-M., Roucou, X. (2021). OpenProt 2021: Deeper functional annotation of the coding potential of eukaryotic genomes. Nucleic Acids Research. DOI
  • Gagnon, M., Savard, M., Jacques, J.-F., Bkaily, G., Geha, S., Roucou, X., Gobeil, F. (2021). Potentiation of B2 receptor signaling by AltB2R, a newly identified alternative protein encoded in the human bradykinin B2 receptor gene. Journal of Biological Chemistry. DOI
  • Cohen, A.A., Leblanc, S., Roucou, X. (2021). Robust Physiological Metrics From Sparsely Sampled Networks. Frontiers in Physiology. DOI
  • Brunet, M.A., Jacques, J.-F., Nassari, S., Tyzack, G.E., McGoldrick, P., Zinman, L., Jean, S., Robertson, J., Patani, R., Roucou, X. (2021). The FUS gene is dual-coding with both proteins contributing to FUS-mediated toxicity. EMBO Reports. DOI
  • Brunet, M.A., Lekehal, A.M., Roucou, X. (2020). How to Illuminate the Dark Proteome Using the Multi-omic OpenProt Resource. Current Protocols in Bioinformatics. DOI
  • Cardon, T., Hervé, F., Delcourt, V., Roucou, X., Salzet, M., Franck, J., Fournier, I. (2020). Optimized Sample Preparation Workflow for Improved Identification of Ghost Proteins. Analytical Chemistry. DOI
  • Brunet, M.A., Leblanc, S., Roucou, X. (2020). Reconsidering proteomic diversity with functional investigation of small ORFs and alternative ORFs. Experimental Cell Research. DOI
  • Dubois, M.-L., Meller, A., Samandi, S., Brunelle, M., Frion, J., Brunet, M.A., Toupin, A., Beaudoin, M.C., Jacques, J.-F., Lévesque, D., Scott, M.S., Lavigne, P., Roucou, X., Boisvert, F.-M. (2020). UBB pseudogene 4 encodes functional ubiquitin variants. Nature Communications. DOI
  • Brunet, M.A., Roucou, X. (2019). Mass spectrometry-based proteomics analyses using the openprot database to unveil novel proteins translated from non-canonical open reading frames. Journal of Visualized Experiments. DOI
  • Brunet, M.A., Brunelle, M., Lucier, J.-F., Delcourt, V., Levesque, M., Grenier, F., Samandi, S., Leblanc, S., Aguilar, J.-D., Dufour, P., Jacques, J.-F., Fournier, I., Ouangraoua, A., Scott, M.S., Boisvert, F.-M., Roucou, X. (2019). OpenProt: A more comprehensive guide to explore eukaryotic coding potential and proteomes. Nucleic Acids Research. DOI
  • Brunet, M.A., Levesque, S.A., Hunting, D.J., Cohen, A.A., Roucou, X. (2018). Recognition of the polycistronic nature of human genes is critical to understanding the genotype-phenotype relationship. Genome Research. DOI
  • Delcourt, V., Staskevicius, A., Salzet, M., Fournier, I., Roucou, X. (2018). Small Proteins Encoded by Unannotated ORFs are Rising Stars of the Proteome, Confirming Shortcomings in Genome Annotations and Current Vision of an mRNA. Proteomics. DOI
  • Delcourt, V., Franck, J., Quanico, J., Gimeno, J.-P., Wisztorski, M., Raffo-Romero, A., Kobeissy, F., Roucou, X., Salzet, M., Fournier, I. (2018). Spatially-Resolved Top-down Proteomics Bridged to MALDI MS Imaging Reveals the Molecular Physiome of Brain Regions. Molecular and Cellular Proteomics. DOI
  • Delcourt, V., Brunelle, M., Roy, A.V., Jacques, J.-F., Salzet, M., Fournier, I., Roucou, X. (2018). The protein coded by a short open reading frame, not by the annotated coding sequence, is the main gene product of the dual-coding gene MIEF1. Molecular and Cellular Proteomics. DOI
  • Delcourt, V., Franck, J., Leblanc, E., Narducci, F., Robin, Y.-M., Gimeno, J.-P., Quanico, J., Wisztorski, M., Kobeissy, F., Jacques, J.-F., Roucou, X., Salzet, M., Fournier, I. (2017). Combined Mass Spectrometry Imaging and Top-down Microproteomics Reveals Evidence of a Hidden Proteome in Ovarian Cancer. Ebiomedicine. DOI
  • Samandi, S., Roy, A.V., Delcourt, V., Lucier, J.-F., Gagnon, J., Beaudoin, M.C., Vanderperre, B., Breton, M.-A., Motard, J., Jacques, J.-F., Brunelle, M., Gagnon-Arsenault, I., Fournier, I., Ouangraoua, A., Hunting, D.J., Cohen, A.A., Landry, C.R., Scott, M.S., Roucou, X. (2017). Deep transcriptome annotation enables the discovery and functional characterization of cryptic small proteins. Elife. DOI
  • Mouilleron, H., Delcourt, V., Roucou, X. (2016). Death of a dogma: Eukaryotic mRNAs can code for more than one protein. Nucleic Acids Research. DOI
  • Landry, C.R., Zhong, X., Nielly-Thibault, L., Roucou, X. (2015). Found in translation: Functions and evolution of a recently discovered alternative proteome. Current Opinion in Structural Biology. DOI
  • Braun, R.J., Dumit, V.I., Monpays, C., Roucou, X., Serrano, D., St-Pierre, J., Waters, P.J., Bates, I., Gris, D. (2015). Struggling for breath in sherbrooke: 1st symposium on “one mitochondrion, many diseases” in Sherbrooke, Québec, Canada, March 11th, 2015. Microbial Cell. DOI
  • Béland, M., Bédard, M., Tremblay, G., Lavigne, P., Roucou, X. (2014). Aβ induces its own prion protein N-terminal fragment (PrPN1)-mediated neutralization in amorphous aggregates. Neurobiology of Aging. DOI
  • H Shott, R., Appanah, C., Grenier, C., Tremblay, G., Roucou, X., Schang, L.M. (2014). Development of kinomic analyses to identify dysregulated signaling pathways in cells expressing cytoplasmic PrP. Virology Journal. DOI
  • Béland, M., Roucou, X. (2014). Taking advantage of physiological proteolytic processing of the prion protein for a therapeutic perspective in prion and Alzheimer diseases. Prion. DOI
  • Roostaee, A., Beaudoin, S., Staskevicius, A., Roucou, X. (2013). Aggregation and neurotoxicity of recombinant α-synuclein aggregates initiated by dimerization. Molecular Neurodegeneration. DOI
  • Bergeron, D., Lapointe, C., Bissonnette, C., Tremblay, G., Motard, J., Roucou, X. (2013). An out-of-frame overlapping reading frame in the ataxin-1 coding sequence encodes a novel ataxin-1 interacting protein. Journal of Biological Chemistry. DOI
  • Vanderperre, B., Lucier, J.-F., Bissonnette, C., Motard, J., Tremblay, G., Vanderperre, S., Wisztorski, M., Salzet, M., Boisvert, F.-M., Roucou, X. (2013). Direct Detection of Alternative Open Reading Frames Translation Products in Human Significantly Expands the Proteome. Plos One. DOI
  • Béland, M., Roucou, X. (2013). Homodimerization as a molecular switch between low and high efficiency PrPc cell surface delivery and neuroprotective activity. Prion. DOI
  • Forget, K.J., Tremblay, G., Roucou, X. (2013). p53 Aggregates Penetrate Cells and Induce the Co-Aggregation of Intracellular p53. Plos One. DOI
  • Vanderperre, B., Lucier, J.-F., Roucou, X. (2012). HAltORF: Adatabase of predicted out-of-frame alternative open reading frames in human. Database. DOI
  • Béland, M., Motard, J., Barbarin, A., Roucou, X. (2012). PrPC homodimerization stimulates the production of PrPC cleaved fragments PrPN1 and PrPC1. Journal of Neuroscience. DOI
  • Vanderperre, B., Roucou, X. (2012). Response. FASEB Journal. DOI
  • Béland, M., Roucou, X. (2012). The prion protein unstructured N-terminal region is a broad-spectrum molecular sensor with diverse and contrasting potential functions. Journal of Neurochemistry. DOI
  • Vanderperre, B., Staskevicius, A.B., Tremblay, G., McCoy, M., O'Neill, M.A., Cashman, N.R., Roucou, X. (2011). An overlapping reading frame in the PRNP gene encodes a novel polypeptide distinct from the prion protein. FASEB Journal. DOI
  • Roostaee, A.R., Roostaee, M.H., Roucou, X. (2010). An update on prion biology and proteomics. Current Proteomics. DOI
  • Beaudoin, S., Vanderperre, B., Grenier, C., Tremblay, I., Leduc, F., Roucou, X. (2009). A large ribonucleoprotein particle induced by cytoplasmic PrP shares striking similarities with the chromatoid body, an RNA granule predicted to function in posttranscriptional gene regulation. Biochimica Et Biophysica Acta Molecular Cell Research. DOI
  • Roostaee, A., Côté, S., Roucou, X. (2009). Aggregation and amyloid fibril formation induced by chemical dimerization of recombinant prion protein in physiological-like conditions. Journal of Biological Chemistry. DOI
  • Roucou, X. (2009). Prion protein and RNA: A view from the cytoplasm. Frontiers in Bioscience. DOI
  • Roucou, X. (2009). Reconstitution of chromatoid body-like particles in cultured cells: A novel approach to elucidate the mechanism of assembly and function of the chromatoid body. RNA Biology. DOI
  • Beaudoin, S., Goggin, K., Bissonnette, C., Grenier, C., Roucou, X. (2008). Aggresomes do not represent a general cellular response to protein misfolding in mammalian cells. BMC Cell Biology. DOI
  • Goggin, K., Beaudoin, S., Grenier, C., Brown, A.-A., Roucou, X. (2008). Prion protein aggresomes are poly(A)+ ribonucleoprotein complexes that induce a PKR-mediated deficient cell stress response. Biochimica Et Biophysica Acta Molecular Cell Research. DOI
  • Goggin, K., Bissonnette, C., Grenier, C., Volkov, L., Roucou, X. (2007). Aggregation of cellular prion protein is initiated by proximity-induced dimerization. Journal of Neurochemistry. DOI
  • Grenier, C., Bissonnette, C., Volkov, L., Roucou, X. (2006). Molecular morphology and toxicity of cytoplasmic prion protein aggregates in neuronal and non-neuronal cells. Journal of Neurochemistry. DOI
  • Bounhar, Y., Mann, K.K., Roucou, X., LeBlanc, A.C. (2006). Prion protein prevents Bax-mediated cell death in the absence of other Bcl-2 family members in Saccharomyces cerevisiae. FEMS Yeast Research. DOI
  • Goggin, K., Roucou, X. (2006). The prion protein does not hesitate to produce aggregates,La protéine prion ne se fait pas prier pour faire des agrégats moléculaires. Medecine Sciences. DOI
  • Roucou, X., Giannopoulos, P.N., Zhang, Y., Jodoin, J., Goodyer, C.G., LeBlanc, A. (2005). Cellular prion protein inhibits proapoptotic Bax conformational change in human neurons and in breast carcinoma MCF-7 cells. Cell Death and Differentiation. DOI
  • Roucou, X., LeBlanc, A.C. (2005). Cellular prion protein neuroprotective function: Implications in prion diseases. Journal of Molecular Medicine. DOI
  • Roucou, X., Gains, M., LeBlanc, A.C. (2004). Neuroprotective Functions of Prion Protein. Journal of Neuroscience Research. DOI
  • Roucou, X., Guo, Q., Zhang, Y., Goodyer, C.G., LeBlanc, A.C. (2003). Cytosolic Prion Protein Is Not Toxic and Protects against Bax-mediated Cell Death in Human Primary Neurons. Journal of Biological Chemistry. DOI
  • Stephens, A.N., Khan, M.A., Roucou, X., Nagley, P., Devenish, R.J. (2003). The molecular neighborhood of subunit 8 of yeast mitochondrial F1F0-ATP synthase probed by cysteine scanning mutagenesis and chemical modification. Journal of Biological Chemistry. DOI
  • Leblanc, A.C., Roucou, X., Priola, S.A., Caughey, B., Chesebro, B. (2003). Toxicity and protection in prions [3] (multiple letters). Science. DOI
  • Zhang, Y., Hong, Y., Bounhar, Y., Blacker, M., Roucou, X., Tounekti, O., Vereker, E., Bowers, W.J., Federoff, H.J., Goodyer, C.G., LeBlanc, A. (2003). p75 neurotrophin receptor protects primary cultures of human neurons against extracellular amyloid β peptide cytotoxicity. Journal of Neuroscience. DOI
  • Roucou, X., Montessuit, S., Antonsson, B., Martinou, J.-C. (2002). Bax oligomerization in mitochondrial membranes requires tBid (caspase-8-cleaved Bid) and a mitochondrial protein. Biochemical Journal. DOI
  • Roucou, X., Rostovtseva, T., Montessuit, S., Martinou, J.-C., Antonsson, B. (2002). Bid induces cytochrome c-impermeable Bax channels in liposomes. Biochemical Journal. DOI
  • Lim, M.L.R., Lum, M.-G., Hansen, T.M., Roucou, X., Nagley, P. (2002). On the release of cytochrome c from mitochondria during cell death signaling. Journal of Biomedical Science. DOI
  • Roucou, X., Martinou, J.-C. (2002). Prolactin and zinc in dialysis patients. Biological Trace Element Research.
  • Roucou, X., Martinou, J.-C. (2001). Conformational change of Bax: A question of life or death. Cell Death and Differentiation. DOI
  • Papakonstantinou, T., Myers, M.A., Jois, J., Roucou, X., Prescott, M., Rowley, M.J., Mackay, I.R. (2001). Expression of protein tyrosine phosphatase-like molecule ICA512/IA-2 induces growth arrest in yeast cells and transfected mammalian cell lines. Journal of Autoimmunity. DOI
  • Roucou, X., Antonsson, B., Martinou, J.-C. (2001). Involvement of mitochondria in apoptosis. Cardiology Clinics. DOI
  • Roucou, X., Martinou, J.-C. (2001). Sponge Bcl-2 homologous protein (BHP2-GC) confers distinct stress resistance to human HEK-293 cells. Cell Death and Differentiation. DOI
  • Roucou, X., Prescott, M., Devenish, R.J., Nagley, P. (2000). A cytochrome c-GFP fusion is not released from mitochondria into the cytoplasm upon expression of Bax in yeast cells. FEBS Letters. DOI
  • Devenish, R.J., Prescott, M., Roucou, X., Nagley, P. (2000). Insights into ATP synthase assembly and function through the molecular genetic manipulation of subunits of the yeast mitochondrial enzyme complex. Biochimica Et Biophysica Acta Bioenergetics. DOI
  • Boyle, G.M., Roucou, X., Nagley, P., Devenish, R.J., Prescott, M. (2000). Modulation at a distance of proton conductance through the Saccharomyces cerevisiae mitochondrial F1F0-ATP synthase by variants of the oligomycin sensitivity-conferring protein containing substitutions near the C-terminus. Journal of Bioenergetics and Biomembranes. DOI
  • Stephens, A.N., Roucou, X., Artika, I.M., Devenish, R.J., Nagley, P. (2000). Topology and proximity relationships of yeast mitochondrial ATP synthase subunit 8 determined by unique introduced cysteine residues. European Journal of Biochemistry. DOI
  • Roucou, X., Artika, I.M., Devenish, R.J., Nagley, P. (1999). Bioenergetic and structural consequences of allotopic expression of subunit 8 of yeast mitochondrial ATP synthase. The hydrophobic character of residues 23 and 24 is essential for maximal activity and structural stability of the enzyme complex. European Journal of Biochemistry. DOI
  • Boyle, G.M., Roucou, X., Nagley, P., Devenish, R.J., Prescott, M. (1999). Identification of subunit g of yeast mitochondrial F1F0-ATP synthase, a protein required for maximal activity of cytochrome c oxidase. European Journal of Biochemistry. DOI
  • Manon, S., Roucou, X., Guérin, M., Rigoulet, M., Guérin, B. (1998). Minireview: Characterization of the yeast mitochondria unselective channel: A counterpart to the mammalian permeability transition pore?. Journal of Bioenergetics and Biomembranes. DOI
  • Roucou, X., Manon, S., Guérin, M. (1997). Conditions allowing different states of ATP- and GDP-induced permeability in mitochondria from different strains of Saccharomyces cerevisiae. Biochimica Et Biophysica Acta Biomembranes. DOI
  • Roucou, X., Manon, S., Guérin, M. (1997). Modulation of the electrophoretic ATP-induced K+-transport in yeast mitochondria by ΔpH. Biochemistry and Molecular Biology International. DOI
  • Roucou, X., Manon, S., Guerin, M. (1995). ATP opens an electrophoretic potassium transport pathway in respiring yeast mitochondria. FEBS Letters. DOI
  • Roucou, X., Manon, S., Guérin, M. (1995). Investigations of the inhibitory effect of propranolol, chlorpromazine, quinine, and dicyclohexylcarbodiimide on the swelling of yeast mitochondria in potassium acetate. Evidences for indirect effects mediated by the lipid phase. Journal of Bioenergetics and Biomembranes. DOI
  • Manon, S., Roucou, X., Rigoulet, M., Guérin, M. (1995). Stimulation of oxidative phosphorylation by electrophoretic K+ entry associated to electroneutral K+/H+ exchange in yeast mitochondria. Bba Bioenergetics. DOI

Chapitres de livre

  • Provencher, N., Leblanc, S., Jacques, J.-F., Roucou, X. (2024). Exploring the Alternative Proteome with OpenProt and Mass Spectrometry. Methods in Molecular Biology. DOI

Autres contributions

Cours enseignés à l'UdeS

  • BCM601 - Biotechnologies et santé humaine. (2024-2026). (3CR).
  • BIM640 - Techniques avancées en génie génétique - Travaux pratiques. (2024-2026). (3CR).
  • BCM540 - Techniques avancées en biochimie. (2023-2025). (4CR).
  • BCM630 - Communication scientifique et collaboration. (2025). (3CR).
  • VIR100 - Virologie humaine appliquée. (2025). (1CR).
  • BCH711 - Biochimie des protéines - Pathologies associées. (2024). (2CR).

Divers

  • Chothani, S., Ruiz-Orera, J., Tierney, J.A.S., Clauwaert, J., Deutsch, E.W., Mar Alba, M., Aspden, J.L., Baranov, P.V., Bazzini, A.A., Bruford, E.A., Brunet, M.A., Cardon, T., Carvunis, A.-R., Casola, C., Choudhary, J.S., Dean, K., Faridi, P., Fierro-Monti, I., Fournier, I., ... Rackham, O.J.L. (2025). An expanded reference catalog of translated open reading frames for biomedical research. Biorxiv.
  • Wacholder, A., Deutsch, E.W., Kok, L.W., van Dinter, J.T., Lee, J., Wright, J.C., Leblanc, S., Jayatissa, A.H., Jiang, K., Arefiev, I., Cao, K., Bourassa, F., Trifiro, F.-A., Bassani-Sternberg, M., Baranov, P.V., Bogaert, A., Chothani, S., Fierro-Monti, I., Fijalkowska, D., ... Carvunis, A.-R. (2025). Detection of human unannotated microproteins by mass spectrometry-based proteomics: a community assessment. Biorxiv.
  • Boisvert, F.-M., Frion, J., Raisch, J., Lévesque, D., Marbach, G., Dubois, M.-L., Meller, A., Roucou, X. (2025). The ubiquitin variant UbKEKS modifies PCNA to enhance DNA polymerase processivity during replication. Research Square.
  • Tristan, C., Rio, D.G.-D., Derhourhi, M., Bonnefond, A., Leblanc, S., Guilloy, N., Roucou, X., Eyckerman, S., Gevaert, K., Salzet, M. (2024). Deciphering the ghost proteome in ovarian cancer cells by deep proteogenomic characterization. Research Square.
  • Çakır, U., Gabed, N., Köroğlu, Y.E., Kaya, S., Sinharoy, S., Benedito, V.A., Brunet, M., Roucou, X., Kryvoruchko, I.S. (2024). Discovery of diverse chimeric peptides in a eukaryotic proteome sets the stage for the experimental proof of the mosaic translation hypothesis. Biorxiv.
  • Deutsch, E.W., Kok, L.W., Mudge, J.M., Ruiz-Orera, J., Fierro-Monti, I., Sun, Z., Abelin, J.G., Mar Alba, M., Aspden, J.L., Bazzini, A.A., Bruford, E.A., Brunet, M.A., Calviello, L., Carr, S.A., Carvunis, A.-R., Chothani, S., Clauwaert, J., Dean, K., Faridi, P., ... van Heesch, S. (2024). High-quality peptide evidence for annotating noncanonical open reading frames as human proteins. Biorxiv.
  • Robitaille, F., Campbell, A., Hadj, A.B., Cornet, S., Nadeau-Lachance, L., Niaison, T., Choquette, T., Nyffeler, L., Roucou, X., Angers, A., Breton, S. (2024). MTALTCO1: a 259 amino-acid long mtDNA-encoded alternative protein that challenges conventional understandings of mitochondrial genomics. Biorxiv.
  • Comtois, F., Jacques, J.-F., Métayer, L., Ouedraogo, W.Y.D.D., Ouangraoua, A., Denault, J.-B., Roucou, X. (2024). Non-canonical altPIDD1 protein: unveiling the true major translational output of the PIDD1 gene. Biorxiv.
  • Meller, A., Lévesque, D., Raisch, J., Fafard-Couture, E., Scott, M., Roucou, X., Boisvert, F.-M. (2024). The Pseudogene RPS27AP5 Reveals Novel Ubiquitin and Ribosomal Protein Variants Involved in Specialised Ribosomal Functions. Biorxiv.
  • Frion, J., Meller, A., Marbach, G., Lévesque, D., Roucou, X., Boisvert, F.-M. (2023). CRISPR/Cas9-mediated knockout of the ubiquitin variant UbKEKS reveals a role in regulating nucleolar structures and composition. Biorxiv.
  • Khatir, I., Brunet, M.A., Meller, A., Amiot, F., Patel, T., Lapointe, X., Lopez, J.A., Guilloy, N., Castonguay, A., Husain, M.A., St-Germain, J., Boisvert, F.-M., Plourde, M., Roucou, X., Laurent, B. (2022). Decoupling of mRNA and protein expression in aging brains reveals the age-dependent adaptation of specific gene subsets. Biorxiv.
  • Kienzle, L., Bettinazzi, S., Brunet, M., Choquette, T., Khorami, H.H., Roucou, X., Landry, C.R., Angers, A., Breton, S. (2022). MTALTND4, a second protein coded by nd4 impacts mitochondrial bioenergetics. Biorxiv.
  • Brunet, M.A., Leblanc, S., Roucou, X. (2022). Openvar: Functional Annotation Of Variants In Non-Canonical Open Reading Frames. Research Square.
  • Mudge, J.M., Ruiz-Orera, J., Prensner, J.R., Brunet, M.A., Gonzalez, J.M., Magrane, M., Martinez, T., Schulz, J.F., Yang, Y.T., Albà, M.M., Baranov, P.V., Bazzini, A., Bruford, E., Martin, M.J., Carvunis, A.-R., Chen, J., Couso, J.P., Flicek, P., Frankish, A., ... van Heesch, S. (2021). A community-driven roadmap to advance research on translated open reading frames detected by Ribo-seq. Biorxiv.
  • Leblanc, S., Brunet, M.A., Jacques, J.-F., Lekehal, A.M., Duclos, A., Tremblay, A., Bruggeman-Gascon, A., Samandi, S., Brunelle, M., Cohen, A.A., Scott, M.S., Roucou, X. (2020). Newfound coding potential of transcripts unveils missing members of human protein communities. Biorxiv.
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