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Sébastien Rodrigue

Professeur, Faculté des sciences

FAC. SCIENCES Biologie

Présentation

Sujet de recherche

Chromosome (Living Organisms), Biological and Biochemical Mechanisms, Genotype and Phenotype, Microorganisms, Bioinformatics

Disciplines de recherche

Biology and Related Sciences

Mots-clés

bacterial gene expression, bioinformatics, genetic engineering, genome sequencing, metagenomics, microbial genetics, single-cell genomics, synthetic biology

Intérêts de recherche

My laboratory develops and applies innovative systems biology, synthetic biology, single-cell genomics, and bioinformatics methodologies for the study and engineering of microorganisms.

Centre de recherche

None

Langues parlées et écrites

Anglais, Français

Diplômes

(2020). (Diploma, Microprogram in business practices). Université de Sherbrooke.

(2010). (Post-doctorate, Post-doctorate fellowship). Massachusetts Institute of Technology.

(2006). (Master's Thesis, M. Sc. in Biology). Université de Sherbrooke.

(2006). (Doctorate, Ph.D. in Biology). Université de Sherbrooke.

(2001). (Bachelor's, B.Sc. in Biology). Université de Sherbrooke.

Expérience académique

Full professor. (2020-). Université de Sherbrooke. Canada.

Associate Professor. (2015-2020). Université de Sherbrooke. Canada.

Assistant Professor. (2010-2015). Université de Sherbrooke. Canada.

Postdoctoral fellow. (2006-2010). Massachusetts Institute of Technology. United States.

Undergraduate research intern. (1999-1999). Université de Sherbrooke. Canada.

Prix et distinctions

  • (2024) Chercheur boursier senior. Fonds de recherche du Québec - Santé (FRQS). (Prize / Award).
  • (2020) Chercheur boursier junior 2. Fonds de recherche du Québec - Santé (FRQS). (Distinction).
  • (2019) Étoile effervescence. Effervescence: le futur des sciences de la vie. (Prize / Award).
  • (2016) Chercheur boursier junior 1. Fonds de recherche du Québec - Santé (FRQS). (Distinction).

Financement

  • Grant. (Awarded). Principal Applicant. Development of an open source platform for synthetic genomics. Natural Sciences and Engineering Research Council of Canada (NSERC). Discovery grant. 210 000 $. (2020-2025)
  • Grant. (Awarded). Principal Applicant. Engineering of a probiotic bacterium for precise microbiome editing. Canadian Institutes of Health Research (CIHR). Project grant. 814 725 $. (2019-2024)
  • Grant. (Awarded). Co-applicant. New approaches to bovine mastitis prevention through microbiome manipulation. Fonds Québécois de la Recherche sur la Nature et les Technologies (FQRNT). Team research project. 225 500 $. (2020-2023)
  • Grant. (Awarded). Co-applicant. Understanding the interplay between Shp-2 and microbiota in colonic inflammation. Canadian Institutes of Health Research (CIHR). Project grant. 100 000 $. (2021-2022)
  • Grant. (Awarded). Co-applicant. Functional analysis of interactions between IncA/C conjugative plasmids and Salmonella Genomic Island 1 that enhance multidrug resistance propagation. Canadian Institutes of Health Research (CIHR). Project grant. 481 950 $. (2017-2022)
  • Grant. (Awarded). Principal Applicant. Identification of conserved synthetic lethality relationships for the development of new antibiotics. Centre de Recherche du Centre Hospitalier de l'Université de Sherbrooke Inc. (CRCHUS) (Sherbrooke, QC). Internal financial assistance program. 25 000 $. (2020-2022)
  • Grant. (Awarded). Principal Applicant. Development of a live biotherapeutic targeting antibiotic-resistant bacteria. Ministère de l'économie et de l'innovation du Québec (MÉI). Programme de soutien aux organisation (PSO). 187 500 $. (2020-2021)
  • Fellowship. (Completed). Principal Applicant. Development of novel technologies for the genomic exploration and modification of the microbiome. Fonds de recherche du Québec - Santé (FRQS). Chercheur boursier Junior 2 - Salary Award. 286 986 $. (2016-2020)
  • Grant. (Completed). Co-applicant. DNA genetic analyzer for molecular ecology/biology applications. Natural Sciences and Engineering Research Council of Canada (NSERC). Research Tools and Instruments. 149 515 $. (2019-2020)
  • Grant. (Completed). Principal Investigator. Development and application of droplet microfluidics for high-throughput single-cell and single-molecule genomics. Natural Sciences and Engineering Research Council of Canada (NSERC). Discovery grant program (individual). 190 000 $. (2015-2020)
  • Grant. (Completed). Principal Applicant. Integrated platform for genome engineering. Fonds Québécois de la Recherche sur la Nature et les Technologies (FQRNT). Team research project. 162 000 $. (2017-2020)
  • Grant. (Completed). Principal Applicant. Engineering of a probiotic bacterium for precise microbiome editing. Canadian Institutes of Health Research (CIHR). Project Grant - Priority Announcement: Antimicrobial Resistance. 100 000 $. (2018-2019)
  • Grant. (Completed). Co-applicant. Development of a hydrogel releasing TRAIL-enriched vesicles that will accumulate in brain cancer cells to increase radiation-induced apoptosis. Centre de Recherche du Centre Hospitalier de l'Université de Sherbrooke Inc. (CRCHUS) (Sherbrooke, QC). Projets structurants 2018 CRCHUS. 75 000 $. (2018-2019)
  • Grant. (Completed). Co-applicant. Development of a hydrogel releasing TRAIL-enriched vesicles that will accumulate in brain cancer cells to increase radiation-induced apoptosis. Fondation du CHUS (Sherbrooke, QC). Projets structurants 2018 CRCHUS. 75 000 $. (2018-2019)
  • Grant. (Completed). Principal Applicant. Creation and characterization of a collection of strains isolated from the human microbiota for the study of the dissemination of resistance to antibiotics. Centre de Recherche du Centre Hospitalier de l'Université de Sherbrooke Inc. (CRCHUS) (Sherbrooke, QC). Axe Inflammation-Douleur. 16 250 $. (2018-2019)
  • Grant. (Completed). Co-applicant. Acquisition of an in vivo imaging system. Natural Sciences and Engineering Research Council of Canada (NSERC). Research Tools and Instruments. 144 824 $. (2017-2018)
  • Grant. (Completed). Principal Investigator. Development and application of droplet microfluidics for high-throughput single-cell and single-molecule genomics. Natural Sciences and Engineering Research Council of Canada (NSERC). Discovery Accelerator Supplements Program. 120 000 $. (2015-2018)
  • Grant. (Completed). Co-applicant. Mechanisms of intestinal contribution to cholesterol gallstones formation. Canadian Institutes of Health Research (CIHR). Project grants. 100 000 $. (2016-2017)
  • Grant. (Completed). Co-applicant. Development of an innovative green biotechnology to valorize cheese production residues. Fonds de recherche du Québec - Nature et technologies (FRQNT). Programme de recherche en partenariat pour l'innovation en production et en transformation laitières. 220 000 $. (2014-2017)
  • Fellowship. (Completed). Principal Investigator. Novel single-cell whole-genome sequencing methods to study genetic variability in microorganisms. Fonds de recherche du Québec - Santé (FRQS). Chercheur boursier Junior 1 - Salary Award. 246 321 $. (2012-2016)
  • Grant. (Completed). Principal Investigator. Novel approaches and methods for bacterial chromosome engineering. Fonds de recherche du Québec - Nature et technologies (FRQNT). Team research projects. 168 000 $. (2013-2016)
  • Grant. (Completed). Principal Applicant. Acquisition of a Fluorescence-Activated Cell Sorter (FACS). Natural Sciences and Engineering Research Council of Canada (NSERC). Research Tools and Instruments Grants Program. 150 000 $. (2015-2016)
  • Grant. (Completed). Co-investigator. Identification of new therapeutic targets for the development of antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). Réseau québécois de recherche sur les médicaments. Identification et validation des cibles thérapeutiques. 25 000 $. (2014-2016)
  • Grant. (Completed). Co-applicant. Imaging system for quantification of DNA, RNA, and proteins. Natural Sciences and Engineering Research Council of Canada (NSERC). Research Tools and Instrumentation. 136 406 $. (2014-2015)
  • Grant. (Completed). Principal Investigator. Mesoplasma florum: a new model for microbial system biology and genome engineering. Natural Sciences and Engineering Research Council of Canada (NSERC). Discovery grant program (individual). 155 000 $. (2010-2015)
  • Grant. (Completed). Principal Investigator. Novel single-cell whole-genome sequencing methods to study genetic variability in microorganisms. Fonds de recherche du Québec - Santé (FRQS). Subvention d'établissement de jeune chercheur. 45 000 $. (2012-2015)
  • Grant. (Completed). Principal Applicant. Novel approaches in genomics and genome engineering. University of Sherbrooke. Subvention de démarrage/Start-up funds. 85 000 $. (2010-2013)
  • Grant. (Completed). Principal Investigator. Functional annotation of the M. florum genome from transcriptomics and proteomics data. Fonds de recherche du Québec - Nature et technologies (FRQNT). Établissement de nouveaux chercheurs. 60 000 $. (2011-2013)
  • Grant. (Completed). Principal Investigator. Infrastructure for project entitled: Functional annotation of the M. florum genome from transcriptomics and proteomics data. Fonds de recherche du Québec - Nature et technologies (FRQNT). Établissement de nouveaux chercheurs. 49 980 $. (2011-2013)
  • Grant. (Completed). Principal Applicant. Data storage unit for high-performance computation in genomics. Natural Sciences and Engineering Research Council of Canada (NSERC). Research Tools and Instruments - Category 1. 14 477 $. (2012-2013)
  • Grant. (Completed). Principal Investigator. Infrastructure for microbial and single-cell genomics. Canada Foundation for Innovation (CFI). Leaders Opportunity Fund. 275 725 $. (2012-2012)
  • Contract. (Completed). Co-investigator. Total understanding of a minimal biological system. Defense Advanced Research Project Agency (The). Living foundries. 500 000 $. (2011-2012)
  • Grant. (Completed). Co-applicant. Acquisition of an Ultra-Centrifuge. Natural Sciences and Engineering Research Council of Canada (NSERC). Research Tools and Instruments Grants Program. 149 806 $. (2011-2011)
  • Grant. (Completed). Co-applicant. DNA Genetic Analyzer. Natural Sciences and Engineering Research Council of Canada (NSERC). Research Tools and Instruments Grants Program. 95 693 $. (2011-2011)
  • Fellowship. (Completed). Principal Applicant. Postdoctoral fellowship NSERC. Natural Sciences and Engineering Research Council of Canada (NSERC). Postdoctoral Fellowship. 80 000 $. (2008-2010)
  • Fellowship. (Awarded). Principal Applicant. Postdoctoral fellowship. Fonds de recherche du Québec - Nature et technologies (FRQNT). Bourse de recherche postdoctorale. 60 000 $. (2006-2007)
  • Scholarship. (Completed). Principal Applicant. Doctoral scholarship FRSQ. Fonds de recherche du Québec - Santé (FRQS). Postgraduate Scholarship (Doctoral). 20 000 $. (2005-2006)
  • Scholarship. (Completed). Principal Applicant. Doctoral scholarship. Natural Sciences and Engineering Research Council of Canada (NSERC). Postgraduate Scholarship (Doctoral). 42 000 $. (2003-2005)
  • Scholarship. (Completed). Principal Applicant. Masters scholarship. Fonds Québécois de la Recherche sur la Nature et les Technologies (FQRNT). Postgraduate Scholarship (Masters). 30 000 $. (2001-2003)
  • Scholarship. (Completed). Masters scholarship. Natural Sciences and Engineering Research Council of Canada. Postgraduate Scholarship (Masters). 37 000 $. (2001-2003)
  • Grant. (Under Review). Co-applicant. Understanding the interplay between Shp-2 and microbiota in colonic inflammation. Crohn's & Colitis Foundation of Canada (CCFC). Grants-in-Aid of Research. 375 000 $.

Publications

Articles de revue

  • Durand, Romain; Huguet, Kévin T; Rivard, Nicolas; Carraro, Nicolas; Rodrigue, Sébastien; Burrus, Vincent. (2021). Crucial role of Salmonella genomic island 1 master activator in the parasitism of IncC plasmids. Nucleic Acids Research 49 (14), 7807-7824. (Published).
  • Lachance, Jean-Christophe; Matteau, Dominick; Brodeur, Joëlle; Lloyd, Colton J; Mih, Nathan; King, Zachary A; Knight, Thomas F; Feist, Adam M; Monk, Jonathan M; Palsson, Bernhard O; Jacques, Pierre-Étienne; Rodrigue, Sébastien. (2021). Genome-scale metabolic modeling reveals key features of a minimal gene set. Molecular Systems Biology 17 (7), e10099. (Published).
  • Neil, Kevin; Allard, Nancy; Roy, Patricia; Grenier, Frédéric; Menendez, Alfredo; Burrus, Vincent; Rodrigue, Sébastien. (2021). High-efficiency Delivery of CRISPR-Cas9 by engineered probiotics enables precise microbiome editing. Molecular Systems Biology 17 (10), e10244. (Accepted).
  • Matteau, Dominick; Rodrigue, Sébastien. (2021). M. pneumoniaeAn engineered to fight. Molecular Systems Biology 17 (10), e10248. (Accepted).
  • Neil, Kevin; Allard, Nancy; Rodrigue, Sébastien. (2021). Molecular Mechanisms Influencing Bacterial Conjugation in the Intestinal Microbiota. Frontiers in Microbiology 12 1415. (Published).
  • Demontier, Elodie;Dubé-Duquette, Alexis; Brouillette, Eric; Larose, Audrey; Ster, Céline; Lucier, Jean-François;Rodrigue, Sébastien; Park, Soyoun; Jung, Dongyun; Ruffini, Janina; Ronholm, Jennifer; Dufour, Simon; Roy, Jean-Philippe; Ramanathan, Sheela; Malouin, François. (2021). Relative virulence of Staphylococcus aureus bovine mastitis strains representing the main Canadian spa types and clonal complexes as determined using in vitro and in vivo mastitis models. Journal of Dairy Science 104 (10), 1-16. (In Press).
  • Neil, K; Allard, N; Grenier, F; Burrus,V; Rodrigue S. (2020). Highly efficient gene transfer in the mouse gut microbiota is enabled by the Incl₂ conjugative plasmid TP114. Communications Biology 3 (1), 1-9. (Published).
  • Matteau, D*; Lachance, JC*; Grenier F, Gauthier, S*; Daubenspeck, JM; Dybvig, K; Garneau, D; Knight, TF; Jacques, PE; Rodrigue S. (2020). Mesoplasma florumIntegrative characterization of the near‐minimal bacterium. Molecular Systems Biology 16 (12), e9844. (Published).
  • Neil K*, Allard N*, Jordan D*, Rodrigue S. (2019). Assembly of Large Mobilizable Genetic Cargo by Double Recombinase Operated Insertion of DNA(DROID). Plasmid 104 102419. (Published).
  • Lachance JC*, Lloyd CJ, Monk JM, Yang L, Sastry AV, Seif Y, Palsson BO, Rodrigue S, Feist AM, King ZA, Jacques PE. (2019). Bofdat: Generating Biomass Objective Functions for Genome-Scale Metabolic Models from Experimental Data. PLoS Comput Biol 15 (4), e1006971. (Published).
  • Baby V, Labroussaa F, Lartigue C, Rodrigue S. (2019). Chromosomes synthétiques - Réécrire le code de la vie [in French]. Medecine/sciences 35 (10), 753-760. (Published).
  • Labroussaa F, Baby V, Rodrigue S, Lartigue C. (2019). La transplantation de génomes - Redonner vie à des génomes bactériens naturels ou synthétiques [in French]. Medecine/sciences 35 (10), 761-770. (Published).
  • Lachance JC*, Rodrigue S, Palsson BO. (2019). Synthetic Biology: Minimal cells, maximal knowledge. eLife 8 e45379. (Published).
  • Larochelle M, Robert MA, Hébert JN, Liu X, Matteau D*, Rodrigue S, Tian B, Jacques PE, Bachand F. (2018). Common mechanism of transcription termination at coding and noncoding RNA genes in fission yeast. Nature Communications 9 (1), 4364. (Published).
  • Baby V*, Lachance JC*, Gagnon J, Lucier JF, Matteau D*, Knight TK, Rodrigue S. (2018). Mesoplasma Inferring the minimal genome of florum by comparative genomics and transposon mutagenesis. mSystems 3 (3), e00198-17. (Published).
  • Lamontagne Boulet M, Isabelle C, Guay I, Brouillette E, Langlois JP, Jacques PE, Rodrigue S, et al. (2018). Tomatidine, a lead antibiotic molecule that targets Staphylococcus aureus ATP synthase subunit C. Antimicrobial Agents and Chemotherapy 62 (6), e02197-17. (Published).
  • Matteau D*, Pepin ME*, Baby V*, Gauthier S*, Arango Giraldo M*, Knight TK, Rodrigue S. (2017). Development of oriC-based plasmids for Mesoplasma florum. Applied and Environmental Microbiology 83 (7), e03374-16. (Published).
  • Baby V*, Labroussaa F, Brodeur J, Matteau D*, Gourgues G, Lartigue C, Rodrigue S. (2017). Mesoplasma florumCloning and transplantation of the genome. ACS Synthetic Biology 7 (1), 209-17. (Published).
  • Meteignier LV, El Oirdi M, Cohen M, Barff T, Matteau D*, Lucier JF, Rodrigue S, Jacques PE, Yoshioka K, Moffett P. (2017). Translatome analysis of an NB-LRR immune response identifies important contributors to plant immunity in Arabidopsis. Journal of Experimental Botany 68 (9), 2333-2344. (Published).
  • Blondeau A, Lucier JF, Matteau D*, Dumont L, Rodrigue S, Jacques PE, Blouin R. (2016). Dual leucine zipper kinase regulates expression of axon guidance genes in mouse neuronal cells. Neural Development 11 (1), 1-13. (Published).
  • Labroussaa F, Lebaudy A, Baby V*, Gourgues G, Matteau D*, Vashee S, Sirand-Pugnet P, Rodrigue S, Lartigue C. (2016). Impact of donor–recipient phylogenetic distance on bacterial genome transplantation. Nucleic Acids Research 44 (17), 8501-8511. (Published).
  • Matteau D*, Baby V*, Pelletier S, Rodrigue S. (2015). A small-volume, low-cost, and versatile continuous culture device. PLoS One 10 (7), e0133384. (Published).
  • Brunelle M, Nordell Markovits A, Rodrigue S, Lupien M, Jacques PE, Gévry N. (2015). The histone variant H2A.Z is an important regulator of enhancer activity. Nucleic Acids Research 43 (20), 9742-56. (Published).
  • Poulin-Laprade D, Matteau D*, Jacques PE, Rodrigue S, Burrus V. (2015). Transfer activation of SXT/R391 integrative and conjugative elements: unraveling the SetCD regulon. Nucleic Acids Research 43 (4), 2045-56. (Published).
  • Carraro N, Matteau D*, Burrus V, Rodrigue S. (2015). Unraveling the regulatory network of IncA/C plasmid mobilization: When genomic islands hijack conjugative elements. Mobile Genetic Elements 5 (3), 1-5. (Published).
  • Grenier F* , Matteau D* , Baby V* , Rodrigue S. (2014). Complete genome sequence of Escherichia coli BW25113. Genome Announcements 2 (5), e01038-14. (Published).
  • Carraro N, Sauvé M*, Matteau D*, Lauzon G, Rodrigue S, Burrus V. (2014). Development of pVCR94deltaX from Vibrio cholerae, a prototype for studying multidrug resistant IncA/C conjugative plasmids. Frontiers in Microbiology 5 (44), 1-13. (Published).
  • Kashtan N, Roggensack SE, Rodrigue S, Thompson JW, Biller SJ, Coe A, Ding H, Marttinen P, Malmstrom RR, Stocker R, Follows MJ, Stepanauskas R, Chisholm SW. (2014). Single-cell genomics reveals hundreds of coexisting subpopulations in wild Prochlorococcus. Science 344 (6182), 416-20. (Published).
  • Carraro N, Matteau D*, Luo P, Rodrigue S, Burrus V. (2014). The master activator of IncA/C conjugative plasmids stimulates genomic islands and multidrug resistance dissemination. PLoS Genetics 10 (10), e1004714. (Published).
  • Zitouni M, Fortin M, Scheerle RK, Letzel T, Matteau D*, Rodrigue S, Brzezinski R. (2013). Biochemical and molecular characterization of a thermostable chitosanase produced by the strain Paenibacillus sp. 1794 newly isolated from compost. Applied Microbiology and Biotechnology 97 (13), 5801-13. (Published).
  • Daccord A, Ceccarelli D, Rodrigue S, Burrus V. (2013). Comparative analysis of mobilizable genomic islands. Journal of Bacteriology 195 (3), 606-14. (Published).
  • Baby V*, Matteau D*, Knight TF, Rodrigue S. (2013). Complete genome sequence of the Mesoplasma florum W37 strain. Genome Announcements 1 (6), e00879-13. (Published).
  • Malmstrom RR, Rodrigue S, Huang KH, Kelly L, Kern SE, Thompson A, Roggensack S, Berube PM, Henn MR, Chisholm SW. (2013). Ecology of uncultured Prochlorococcus clades revealed through single-cell genomics and biogeographic analysis. The ISME Journal 7 (1), 184-98. (Published).
  • Waldbauer JR, Rodrigue S, Coleman ML, Chisholm SW. (2012). Transcriptome and proteome dynamics of a light-dark synchronized bacterial cell cycle. PloS One 7 (8), e43432. (Published).
  • Rodrigue S, Materna AC, Timberlake SC*, Blackburn MC*, Malmstrom RR, Alm EJ, Chisholm SW. (2010). Unlocking short read sequencing for metagenomics. PLoS One 5 (7), e11840. (Published).
  • Wu S , Barnes PF , Samten B , Pang X , Rodrigue S , Ghanny S , Soteropoulos P , Gaudreau L , Howard ST. (2009). Activation of the eis gene in a W-Beijing strain of Mycobacterium tuberculosis correlates with increased SigA levels and enhanced intracellular growth. Microbiology (Reading, England) 155 (4), 1272-81. (Published).
  • Donà V* , Rodrigue S* , Dainese E , Palù G , Gaudreau L , Manganelli R , Provvedi R. (2008). Evidence of complex transcriptional, translational, and posttranslational regulation of the extracytoplasmic function sigma factor sigmaE in Mycobacterium tuberculosis. Journal of bacteriology 190 (17), 5963-71. (Published).
  • Rodrigue S , Brodeur J , Jacques PE , Gervais AL , Brzezinski R , Gaudreau L. (2007). Identification of mycobacterial sigma factor binding sites by chromatin immunoprecipitation assays. Journal of bacteriology 189 (5), 1505-13. (Published).
  • Kettler GC , Martiny AC , Huang K , Zucker J , Coleman ML , Rodrigue S , Chen F , Lapidus A , Ferriera S , Johnson J , Steglich C , Church GM , Richardson P , Chisholm SW. (2007). Patterns and implications of gene gain and loss in the evolution of Prochlorococcus. PLoS genetics 3 (12), e231. (Published).
  • Sureka K , Dey S , Datta P , Singh AK , Dasgupta A , Rodrigue S , Basu J , Kundu M. (2007). Polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria. Molecular microbiology 65 (2), 261-76. (Published).
  • Jacques JF , Rodrigue S , Brzezinski R , Gaudreau L. (2006). A recombinant Mycobacterium tuberculosis in vitro transcription system. FEMS microbiology letters 255 (1), 140-7. (Published).
  • Jacques PE , Rodrigue S , Gaudreau L , Goulet J , Brzezinski R. (2006). Detection of prokaryotic promoters from the genomic distribution of hexanucleotide pairs. BMC bioinformatics 7 423. (Published).
  • Dainese E , Rodrigue S , Delogu G , Provvedi R , Laflamme L , Brzezinski R , Fadda G , Smith I , Gaudreau L , Palù G , Manganelli R. (2006). Posttranslational regulation of Mycobacterium tuberculosis extracytoplasmic-function sigma factor sigma L and roles in virulence and in global regulation of gene expression. Infection and immunity 74 (4), 2457-61. (Published).
  • Rodrigue S , Provvedi R , Jacques PE , Gaudreau L , Manganelli R. (2006). The sigma factors of Mycobacterium tuberculosis. FEMS microbiology reviews 30 (6), 926-41. (Published).
  • Manganelli R , Provvedi R , Rodrigue S , Beaucher J , Gaudreau L , Smith I , Proveddi R. (2004). Sigma factors and global gene regulation in Mycobacterium tuberculosis. Journal of bacteriology 186 (4), 895-902. (Published).
  • Beaucher J , Rodrigue S , Jacques PE , Smith I , Brzezinski R , Gaudreau L. (2002). Novel Mycobacterium tuberculosis anti-sigma factor antagonists control sigmaF activity by distinct mechanisms. Molecular microbiology 45 (6), 1527-40. (Published).

Chapitres de livre

  • Jean-Christophe Lachance, JC; Rodrigue, S; Palsson BO. (2020). The Use of In Silico Genome-Scale Models for the Rational Design of Minimal Cells. Lara, AR; Gosset G. Minimal Cells: Design, Construction, Biotechnological Applications (141-175). Switzerland : Springer. (Published).
  • Brunelle M, Rodrigue S, Jacques PE, Gévry N. (2017). High-resolution genome-wide mapping of nucleosome positioning and occupancy level using paired-end sequencing technology. Guillemette B, Gaudreau L. Histones: Methods and Protocols (229-243). United States : Springer. (Published).
  • Bianco S, Rodrigue S, Murphy BD, Gévry N. (2015). Global mapping of open chromatin regulatory elements by formaldehyde-assisted isolation of regulatory elements followed by sequencing (FAIRE-seq). Leblanc B, Rodrigue S. Methods in Molecular Biology (1334, 261-72). Switzerland : Springer. (Published).
  • Matteau D*, Rodrigue S. (2015). Identification of transcription start sites by genome-wide 5’-Rapid Amplification of cDNA Ends (5’-RACE). Leblanc B, Rodrigue S. Methods in Molecular Biology (4, 1-18). United States : Springer. (Published).
  • Matteau D*, Rodrigue S. (2015). Precise identification of DNA-binding proteins genomic location by exonuclease coupled chromatin immunoprecipitation (ChIP-exo). Leblanc B, Rodrigue S. Methods in Molecular Biology (1334, 173-93). Switzerland : Springer. (Published).
  • Grenier F*, Lucier JF, Rodrigue S. (2015). Selection and validation of spacer sequences for CRISPR- Cas9 genome editing and transcription regulation in bacteria. Leblanc B, Rodrigue S. Methods in Molecular Biology; DNA protein interactions. (233-244). United Kingdom : Springer. (Published).

Propriétés intellectuelles

Patents

  • Sébastien Rodrigue, Kevin Neil, Nancy Allard, Vincent Burrus. Probiotic bacterial conjugative system and therapeutic uses thereof. 62/696,367. United States. (Pending).

Autres contributions

Gestion d'évènements

  • Scientific committee member. (2019) 69th Annual Conference of the Canadian Society of Microbiologists. (Conference).
  • Organizing committee member. (2019) Inno-Santé 360. (Conference).
  • Organizing committee member. (2018) RECOMB Comparative Genomics 2018. (Conference).

Activités de collaboration internationale

  • Collaborator. United States. Collaboration with Tom Knight (GinkgoBioworks), specialist in synthetic biology.
  • Collaborator. France. Ongoing collaboration with Pr Carole Lartigue laboratory at INRA for genome transplantation and synthetic biology.
  • Collaborator. France. Collaboration with Eric Bapteste laboratory for genome sequencing of genomes from individual cells containing genetic markers of interest.
  • Collaborator. United States. Collaboration with Bernhard O. Palsson laboratory at UCSD for in silico modelling of minimal genome.

Présentations

  • (2021). Learning genome design rules using the near-minimal bacterium Mesoplasma florum. Synbio Canada webinar series. Canada
  • (2020). Using synthetic biology to engineer microbial genomes and to edit microbiomes. 5e rencontre du Club d'édition génomique de l'Université Laval. Québec, Canada
  • (2019). A synthetic biology platform to engineer live biotherapeutics. Montreal In vivo annual general meeting. Montreal, Canada
  • (2019). Comprendre pour créer et créer pour comprendre : la biologie synthétique comme plate-forme d'innovation. Effervescence 2019 : le futur des sciences de la vie. Montreal, Canada
  • (2019). Engineering of a probiotic bacterium for microbiome editing. 69th Annual Conference of the Canadian Society of Microbiologists. Sherbrooke, Canada
  • (2019). Engineering of a probiotic bacterium for precise microbiome editing. Conférence du département de biochimie. Sherbrooke, Canada
  • (2019). Ingénierie d'un probiotique pour manipuler la composition du microbiote. 61e réunion annuelle du CRCQ (Club de Recherches Cliniques du Québec). Estérel, Canada
  • (2019). Programmer des probiotiques pour éliminer les bactéries pathogènes. Journée scientifique de l'axe et du thème Inflammation-Douleur. Sherbrooke, Canada
  • (2018). Bacterial synthetic genomics: possible contributions to the GP-write project. Genome Project (GP)-write Canada Meeting. Montreal, Canada
  • (2018). Développement d’une plateforme technologique pour la caractérisation et l’ingénierie de cellules quasi-minimales. Université de Bordeaux. Bordeaux, France
  • (2018). Inferring the minimal genome of Mesoplasma florum by comparative genomics and transposon mutagenesis. 22nd Congress of the International Organization for Mycoplasmology, Portsmouth, New Hampshire. Portsmouth, New Hampshire, United States
  • (2017). Exploiting minimal genomes as models for systems and synthetic biology. Invited presentation, Department of bioengineering, University of California in San Diego, La Jolla, California. United States
  • (2017). From systems biology to synthetic biology: new approaches to fight bacterial pathogens. Journée scientifique cancer-inflammation-douleur. Orford, Canada
  • (2017). Investigation of whole-genome cloning and genome transplantation using Mesoplasma florum. Invited presentation, Gingko Bioworks Inc., Boston, Massachussetts. United States
  • (2017). Whole-genome cloning and genome transplantation using the quasi-minimal bacterium Mesoplasma florum. Invited presentation, Concordia University, Montreal. Canada
  • (2016). Integrating high-throughput omics for systems and synthetic biology. Forum technologique Novalait 2016. Drummondville, Canada
  • (2015). Transplanting bacterial genomes: changing one species into another. Rendez-vous Génome Québec. Sherbrooke, Canada