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Antonio Conconi

Professeur, Faculté de médecine et des sciences de la santé

FMSS Dép. de microbiologie et infectiologie

Présentation

Sujet de recherche

Chromosomes: structure / organisation, Dommages et réparation du génome, Expression et régulation génique, Gènes, Génétique moléculaire

Disciplines de recherche

Biologie cellulaire, Biologie moléculaire

Mots-clés

Chromatine, Gènes ribosomaux, Lésion de l¿ADN, Méthodes de bio. cell., Méthodes de bio. mol., Microscopie électronique, Réparation de l¿ADN, Transcription

Intérêts de recherche

L’exposition d’organisme à des agents environnementaux tels que les rayons solaires ultraviolet, les rayons ionisants et de nombreux produits génotoxiques, cause des dommages à l’ADN. S’ils ne sont réparés, ces dommages induisent l’apparition de mutations et de cancer. Ainsi, la stabilité du génome est importante dans la prévention de l’oncogenèse.

Centre de recherche

Centre de recherche du CHUS

Langues parlées et écrites

Allemand, Anglais, Français, Italien

Diplômes

(1995). n.a. (Post-doctorat, Postdoctorat). Washington State University.

(1988). n.a. (Post-doctorat, Postdoctorat). Swiss Federal Institute of Technology (ETH).

(1987). Correlation between gene activity and chromatin structure in mouse erythroleukemia cells (Doctorat, Doctorat (Ph.D.)). Swiss Federal Institute of Technology (ETH).

(1983). Psoralen crosslinking von loeslichem und H1-freiem loeslichem rattenleberchromatin (Diplôme, Diplome ETH-Zurich). Swiss Federal Institute of Technology (ETH).

Titres de compétence

(2012) Directeur de l'Axe Oncologie du CRC (Centre de Recherche Clinique, Sherbrooke). Université de Sherbrooke.

(2011) Directeur des Programmes de Microbiologie (Faculté de médecine, Université de Sherbrooke). Université de Sherbrooke.

(2003) Habilitation.

Publications

Articles de revue

  • Peyresaubes F, D'Amours A, Leduc F, Grégoire MC, Boissonneault G, Conconi A. (2015). Immuno-capture of UVDE generated 3'-OH ends at UV photoproducts. DNA Repair
  • Guintini L, Charton R, Peyresaubes F, Thoma F, Conconi A. (2015). Nucleosome positioning, nucleotide excision repair and photoreactivation in Saccharomyces cerevisiae. DNA Repair
  • Charton R, Guintini L, Peyresaubes F, Conconi A. (2015). Repair of UV induced DNA lesions in ribosomal gene chromatin and therole of "Odd" RNA polymerases (I and III). DNA Repair
  • Maxime Tremblay, Romain Charton, Manuel Wittner, Geneviève Levasseur, Joachim Griesenbeck & Antonio Conconi1,. (2013). UV light induced DNA lesions cause dissociation of yeast RNA polymerases-I and establishment of a specialized chromatin structure at rRNA genes. Nuclei Acids Res (Révisions requises).
  • Toussaint M, Wellinger RJ and Conconi A. (2010). Differential participation of homologous recombination and nucleotide excision repair in yeast survival to ultraviolet light radiation. Mutat Res 698 52-59. (Article publié).
  • Tremblay M, Toussaint M, D’Amours A and Conconi A. (2009). Nucleotide excision repair and photolyase repair of UV photoproducts in nucleosomes; assessing the existence of nucleosome and non-nucleosome rDNA chromatin in vivo. Biochem. Cell Biol. 87 337-346. (Article publié).
  • Tremblay M, Teng Y, Paquette M, Waters R and Conconi A. (2008). Complementary roles of yeast Rad4p and Rad34p in nucleotide excisionrepair of active and inactive rRNA gene chromatin (cited 20 times). Mol. Cell Biol. 28 7504-7513.
  • Catala M, Tremblay M, Samson E, Conconi A and Abu Elela S. (2008). Deletion of Rnt1p alters the proportion of open versus closed rRNA generepeats in yeast. Mol. Cell Biol. 28 619-629.
  • Conconi A. (2007). The yeast rDNA locus: a model system to study DNA repair in chromatin (cited 18 times). DNA Repair 4 897-908.
  • Toussaint M, Levasseur G, Gervais-Bird J, Wellinger RJ, Abou Elela S and Conconi A. (2006). A high throughput method to measure the sensitivity of yeast cells togenotoxic agents in liquid cultures (cited 22 times). Mutat. Res. 606 92-105.
  • Toussaint M and Conconi A. (2006). High-throughput and sensitive assay to measure yeast cell growth: abench protocol for testing genotoxic agents (cited 35 times). Nature protocol 1 1922-1928.
  • Toussaint M, Levasseur G, Tremblay M, Paquette M and Conconi A. (2005). Psoralen photo-crosslinking: a useful tool to study the structure ofrDNA chromatin (cited 26 times). Biochem. Cell Biol. 83 449-459.
  • Fahy D, Conconi A and Smerdon MJ. (2005). Rapid changes in transcription and chromatin structure of ribosomalgenes in yeast during growth phase transitions (cited 17 times). Exp. Cell Res. 305 365-373.
  • Conconi A*, Paquette M, Bespalov V, Fahy D and Smerdon MJ (*corresponding author). (2005). Repair-independent chromatin assembly onto active ribosomal genes inyeast after UV irradiation (cited 17 times). Mol. Cell Biol. 25 9773-9783.
  • Conconi A and Wellinger RJ. (2003). A New link for a Linker Histone. Mol Cell 11 1421-1423.
  • Conconi A, Bespalov VA and Smerdon MJ. (2002). Transcription-coupled repair occurs in RNA polymerase I-transcribedgenes in yeast (cited 74 times). Proc. National Acad. Sci. USA (PNAS) 99 649-654.
  • Bespalov VA, Conconi A, Zhang X, Fahy D and Smerdon MJ. (2001). Improved Method for Measuring the Ensemble Average of Strand Breaks inGenomic DNA (cited 15 times). Environ. Mol. Mutagen. 38 166-174.
  • Conconi A, Jager-Vottero P, Zhang X, Beard BC and Smerdon MJ. (2000). Mitotic Viability and Metabolic Competence in UV-Irradiated Yeast Cells (cited 18 times). Mutat. Res. 459 55-64.
  • Conconi A, Liu X, Koriazova L, Ackerman EJ and Smerdon, MJ. (1999). Tight Correlation Between Inhibition of DNA Repair In Vitro andTranscription Factor IIIA Binding in the 5S Ribosomal Gene (cited 18 times). EMBO J. 18 1387-1396.
  • Conconi A. (1997). DNA Damage Can Alter the Stability of Nucleosomes: Effects Are Dependenton Damage Type. Chemtracs, Biochemistry and Molecular Biology 10 1018-1024.
  • Liu X, Conconi A and Smerdon MJ. (1997). Strand Specific Modulation of UV Photoproducts in 5S rDNA by TFIIIABinding and Their Effect on TFIIIA Complex Formation (cited 24 times). Biochemistry 36 13710-13717.
  • Conconi A, Miquel M, Browse JA and Ryan CA. (1996). Intracellular Levels of Free Linolenic and Linoleic Acids Increase inTomato Leaves in Response to Wounding (cited 209 times). Plant Phys. 111 797-803.
  • Conconi A, Smerdon MJ, Howe GA and Ryan CA. (1996). The Octadecanoid Signaling Pathway in Plants Mediates a Response toUltraviolet Radiation (cited 230 times). Nature 383 826-829.
  • Doares SH, Narvaez J, Conconi A and Ryan CA. (1995). Salicylic Acid Inhibits Synthesis of Proteinase Inhibitors in TomatoLeaves Induced by Systemin and Jasmonic Acid (cited 508 times). Plant Phys. 108 1741-1746.
  • Conconi A and Ryan CA. (1993). DNAseI and Micrococcal Nuclease Analysis of the Tomato ProteinaseInhibitor-I Gene in Chromatin (cited 20 times). J. Biol. Chem. 268 430-435.
  • Conconi A, Sogo JM and Ryan CA. (1992). Ribosomal Gene Clusters are Uniquely Proportioned Between Open andClosed Chromatin Structures in Both Tomato Leaf Cells and Exponentially GrowingSuspension Cultures (cited 46 times). Proc. National Acad. Sci. USA (PNAS) 89 5256-5260.
  • Conconi A, Widmer RM, Koller T and Sogo JM. (1989). Two Different Chromatin Structures Coexist in Ribosomal RNA GenesThroughout the Cell Cycle (cited 302 times). Cell 57 753-761.
  • Conconi A, Losa R, Koller T and Sogo JM. (1984). Psoralen-Crosslinking of Soluble and H1-Depleted Soluble Rat LiverChromatin (cited 37 times). J. Mol. Biol. 178 920-928.

Numéros de revue

  • Conconi A. (2015). DNA repair in Chromatin. DNA Repair

Chapitres de livre

  • Grisenbeck J, Wittner M, Charton R and Conconi A. (2012). Chromatin Endogenous cleavage and psoralen crosslinking assays to analyze rRNA gene chromatin in vivo. In: Transcriptional Regulation: Methods and Protocols. Methods Mol Biol (809, 291-301). Humana press NY. (Article publié).
  • Pelloux J, Tremblay M, Wellinger RJ and Conconi A. (2012). UV induced DNA damage and DNA repair in ribosomal genes chromatin. In: Transcriptional Regulation: Methods and Protocols. Methods Mol Biol (809, 303-320). Humana press NY. (Article publié).
  • Conconi A. (2007). Yeast as a model system to study DNA damage and DNA repair (cited 19 times). Sourcebook of Models for Biomedical Research (Humana Press, Totowa NJ, 445-453). P.M. Conn.
  • Conconi A, Bespalov VA, Fahy D and Smerdon MJ. (2005). DNA Repair in RNA Polymerase I-transcribed Genes. From DNA photolesions to mutations, skin cancer and cell death; Comprehensive Series in Photosciences (RS-C Publishers, Royal Society of Chemistry, 123-147). E. Sage, R. Drouin, and M. Rouabhia.
  • Smerdon MJ and Conconi A. (1999). Modulation of DNA Damage and Repair in Chromatin (cited 111 times). Progress in Nucl Acids Res and Mol Biol (Academic Press, 62, 227-255). K. Moldave.
  • Sogo JM, Conconi A and Widmer RM. (1989). The Use of Psoralen-Photocrosslinking for the Analysis of the Chromatin Structure During Transcription. Photochemical Probes in Biochemistry (Kluwer Academic Publishers, 179-194). Nielsen, P.E.

Articles de conférence

  • Guintini L, Tremblay M, Toussaint M, D’Amours A, Wellinger R, Wellinger RJ and Conconi A. (2015). Repair of UV-induced DNA lesions is modulated by nucleosomes and tempered by Sir proteins in subtelomeric chromatin of Saccharomyces cerevisiae (). 37th Asilomar Chromatin and Chromosomes Conference. USA.
  • Charton R and Conconi A. (2012). The proportion of non-nucleosomal vs. nucleosomal rRNA genes changes in yeast cells arrested at the G1/S phase of the cell cycle by hydroxy urea. 55th Annual CSMB Conference, Whistler, Canada. (Article publié).
  • Tremblay M, Levasseur G and Conconi A. (2012). UV induced DNA lesions inhibit transcription of ribosomal genes by displacing RNA polymerase I, which trigger the deposition of nucleosomes (). 55th Annual CSMB Conference, Whistler, Canada. (Article publié).
  • Pelloux J, Tremblay M, Wellinger R, and Conconi A. (2010). UV induced DNA damage and nucleotide excision repair in chromatin: the search for entry and the search for blocking it. Clinical Oncology Research Initiative. Québec, Canada. (Article publié).
  • Tremblay M, Levasseur G, and Conconi A. (2009). The fate of stalled RNA polymerase-I at UV induced DNA damage, an apoptotic signal?. Clinical Oncology Research Initiative. Québec, Canada. (Article publié).
  • Tremblay M, Charton R, French SL, Beyer AL and Conconi A. (2009). UV induced DNA lesions in the transcribed DNA strand trigger the displacement of RNA polymerase I. Clinical Oncology Research Initiative. Québec, Canada. (Article publié).
  • Pelloux J, Charton R, Toussaint M, Wellinger R, Gravel S, Wellinger R and Conconi A. (2009). Repair of UV induced DNA lesions in the heterochromatic chromatin structure of yeast telomeres. 7th Canadian Telomeres and Telomerase Symposium, Canada. (Article publié).
  • Catala M, Tremblay M, Samson E, Conconi A and Abou Elela S. A new linkbetween rRNA transcription and processing in yeast. The 6th eastern Ribo-Club, Canada.
  • Toussaint M, Tremblay M, Paquette M, Wellinger R and Conconi A. DNArepair of UV damage in yeast; effect of SIR2 deletion (). Annual West Coast Chromatin and Chromosomes Meeting, USA.
  • Toussaint M, Dyon J, Wellinger RJ and Conconi A. DNA repair of UV damage inyeast; effect of Sir deletion. FASEB Summer research conferences; chromatin and transcription, USA.
  • Catala M, Tremblay M, Samson E, Conconi A and Abou Elela S. Deletion ofyeast RNaseIII alters the chromatin structure of rRNA genes. 11th Annual Meeting of the RNA society, USA.
  • Catala M, Tremblay M, Samson E, Conconi A and Abou Elela S. Deletion ofyeast RNaseIII alters the chromatin structure of rRNA genes. The 7th eastern Ribo-Club, Canada.
  • Charton R, Griesenbeck J, Smerdon MJ and Conconi A. Hydroxyurea-arrested yeast cell grow greatly affects transcription and chromatin structure of rRNA genes (). Asilomar Chromatin and Chromosomes Conference. USA.
  • Catala M, Tremblay M, Samson E, Conconi A and Abou Elela, S. Novel cellular rolesfor yeast ribonuclease Rnt1p. The 5th Eastern Ribo-Club opening session, Canada.
  • Tremblay M, Teng Y, Paquette M, Waters R and Conconi A. Nucleotide excision repairin active (non-nucleosome) and inactive (nucleosome) rDNA chromatin (). Asilomar Chromatin and Chromosomes Meeting, USA.
  • Tremblay M and Conconi A. Psoralen photo-crosslinking, a tool to separate and analyze active ribosomalgenes in wt yeast and in chromatin remodeling mutants. The 5th Eastern Ribo-Club, Canada.
  • Guintini L, Tremblay M, Toussaint M, Wellinger R and Conconi A. Repair of UV induced DNA lesions in natural sub-telomeric chromatin. Canadian Symposium on Telomeres and Genome Integrity, London Ontario.
  • Pelloux J, Charton R, Toussaint M, Wellinger R, Gravel S, Wellinger R and Conconi A. Repair of UV induced DNA lesions in the heterochromatic chromatin structure of yeast telomeres. 7th Canadian Telomeres and Telomerase Symposium, Canada.
  • Toussaint M, Wellinger RJ and Conconi A. Sir-mutants and nucleotide excision repairon telomeres of yeast (). 6th Canadian Telomeres and Telomerase Symposium.
  • Toussaint M, Wellinger RJ and Conconi A. Sir-mutants and nucleotide excision repairon telomeres of yeast (). 6th Canadian Telomeres and Telomerase symposium.
  • Guintini L, Tremblay M, Toussaint M, Wellinger R and Conconi A. Spotlight on repair of UV induced DNA lesions on natural chromosomeends (). 2nd Canadian Symposium on telomeres and Genome Integrity. Quebec.
  • Tremblay M, Levasseur G, and Conconi A. The fate of stalled RNA polymerase-I at UV induced DNA damage, anapoptotic signal?. Clinical Oncology Research Initiative. Québec.
  • Charton R, Tremblay M, Zeladon C, Peyresaubes F, Guintini L, Conconi A. The nucleolus and its role in cell growth control; jamming rRNAsynthesis (). DNA Repair in Chromatin: The First 40 years (and Beyond); p.14. 1st Biennial Chromatin DNA-Repair, Lecture at Washington State University. USA.
  • Conconi A. The research in Conconi’s lab. Fourth annual new principal investigatorsmeeting. Institutes of Genetics and Institute of human development, child and youth health, CIHR.
  • Conconi A, Bespalov V and Smerdon MJ. Transcription-Coupled Repair and ChromatinRemodeling in Ribosomal Genes of Yeast (). DNA Repair: Interplay with other Cellular Processes, The Netherlands.
  • Conconi A and Paquette M. UV-induced DNA lesions trigger nucleosomesdeposition onto active rDNA in the absence of the damage recognition proteinRad14 (. Annual West Coast Chromatin and Chromosomes Meeting, USA.
  • Conconi A, Tremblay M, Charton R, Wittner M, Zeladon-Orellana CJ, Guintini L, Levasseur G and Griesenbeck J. UV induced DNA damage causes dissociation of yeast RNA polymerase-I followed by deposition of nucleosomes: nucleotide excision repair along the rRNA gene chromatin. A Wellcome Trust Scientific Conference, Hinxton UK.
  • Tremblay M, Charton R, French SL, Beyer AL and Conconi A. UV induced DNA lesions in the transcribed DNA strand trigger the displacement of RNA polymerase I. Clinical Oncology Research Initiative. Québec.
  • Conconi A. UV induced DNA lesions trigger displacement of RNA polymerase I and nucleosomes deposition onto active ribosomal genes (). 3rd Annual World Congress of Molecular & Cell Biology, Suzhou, China.
  • Tremblay M, Levasseur G, French SL, Beyer AL and Conconi A. UV induced DNA lesions trigger nucleosome deposition on active ribosomal genes: the fate of RNA polymerase I (). 7th International Conference on RNA Polymerase I and II. Warrenton, USA.

Autres contributions

Gestion d'évènements

  • Committee director. 10 years evaluation of the Microbiology Programs, Univ de Sherbrooke. (Association).
  • Committee member. Committee member, 10 years evaluation of Physiology Programs, Univ de Sherbrooke. (Association).
  • Committee member. Committee member, Structural planning of a new Research Institute, School of Medicine, Uiv de Sherbrooke. (Association).
  • Director of the Habilitation Committee. Director of the Habilitation Committee, Department of Microbiology, Univ de Sherbrooke. (Association).
  • Director of the Oncology Research Group. Director of the Oncology Research Group, College of Medicine, Univ de Sherbrooke. (Association).
  • Director of the Programs in Microbiology. Director of the Programs in Microbiology, Univ de Sherbrooke. (Association).
  • Committee member. Laboratory safety committee, Faculté de médecine Université de Sherbrooke. (Atelier).

Activités de collaboration internationale

  • Research collaboration. Dr R. Waters, Welsh Medical School, Cardiff University, UK.
  • Research collaboration. Dr M.J. Smerdon, Biochemistry and Biophysics, Washington State University, USA.
  • Research Collaboration. 2008-ongoing Dr J. Griesenbeck, Department of Biochemistry III, Universityof Regensburg, Germany Sponsored by Le Ministère des Relations Internationales du Québec.
  • Research collaboration. 2009 - ongoing Dr R. Wellinger, Centro Andaluz de Biología Molecular y Medicina Regenerativa,Spain.
  • Research Collaboration. 2012 - ongoing Dr M.J. Smerdon, Department Biochemistry and Biophysics, Washington State University, USA.

Présentations

  • (2010). Nucleotide Excision repair in the rDNA locus. Department of Biochemistry, University of Alberta, Edmonton - 2010. Edmonton
  • (2010). The fate of RNA polymerase I at DNA lesions. Department of Biochemistry III, University of Regensburg, Regensburg, Germany. Regensburg
  • (2010). UV induced DNA lesions trigger nucleosomes deposition on the active ribosomal genes - The fate of RNA polymerase I -. 7th International Conference on RNA Polymerase I and III, Warrenton, USA. Warrenton
  • (2009). The fate of RNA polymerase I at DNA lesion. Province of Alberta Genome Instability Meeting, University of Calgary, Calgary - 2009. Calgary
  • Nurcleotide excision repair and the fate of RNA polymerase I co-chair. 3rd Annual World Congress of Molecular and Cell Biology, Suzhou, China - 2013.
  • The fate of RNA polymerase I at UV induced DNA lesions. Department of Biochemistry III, University of Regensburg, Regensburg, Germany - 2014.
  • The yeast nucleolus: DNA damage and DNA repair chair. DNA Repair in Chromatin: The First 40 years (and Beyond). 1st Biennial Chromatin DNA-Repair, Lecture at Washington State University. USA - 2015.
  • UV-induced DNA lesions trigger chromatin assembly on the active ribosomal genes in yeast. School of Molecular Biosciences, Washington State University, Pullman, USA - 2005.
  • UV-induced DNA lesions trigger chromatin assembly on the active ribosomal genes in yeast. Département de Microbiologie et Immunologie, Université de Montreal - 2005.
  • UV-induced DNA lesions trigger chromatin assembly on the active ribosomal genes in yeast. In search for the role of Rad4p during NER of active and inactive rDNA chromatin. Centre de Recherche de L’Hotel-Dieu de Québec. Université de Laval - 2005.