Jean-Bernard Denault

Financement

• Subvention. Imaging protein crystals for identification of submicron nucleation and growth optimization. Natural Sciences and Engineering Research Council (Ottawa, Canada). 122 917 $. (2021-2022).
  Numéro de subvention : RTI-2022-00252. Voir plus
• Subvention. The Caspase Proteolytic System. Natural Sciences and Engineering Research Council (Ottawa, Canada). 26 000 $. (2020-2021).
  Numéro de subvention : RGPIN-2017-05988. Voir plus
• Subvention. Non-canonical role of initiator caspases in colorectal cancer. Canadian Institutes of Health Research (Ottawa, Canada). 683 338 $. (2019-2024).
  Numéro de subvention : 201809PJT. Voir plus
• Subvention. HARNESSING PEPTIDASE'S ACTIVE SITE TO DISRUPT PROTEIN INTERACTION. Natural Sciences and Engineering Research Council (Ottawa, Canada). 12 500 $. (2015-2016).
  Numéro de subvention : 486965-2015. Voir plus
• Subvention. Harnessing peptidase's active site to disrupt protein interaction. Natural Sciences and Engineering Research Council (Ottawa, Canada). 25 000 $. (2014-2015).
  Numéro de subvention : 469488-2014. Voir plus
• Subvention. Development of robust and flexible assays for PARP-1. Natural Sciences and Engineering Research Council (Ottawa, Canada). 11 500 $. (2014-2015).
  Numéro de subvention : 476588-2014. Voir plus
• Subvention. Comprendre et exploiter les exosites des caspases. Fonds de Recherche du Québec – Nature et Technologies (Montreal, Canada). 177 444 $. (2013-2016).
  Numéro de subvention : 171852. Voir plus
• Subvention. Mécanismes d'activation, de régulation et de l'activité des caspases. Fonds de Recherche du Québec - Santé (Montreal, Canada). 230 053 $. (2013-2016).
  Numéro de subvention : 26796. Voir plus
• Subvention. An institutional LC-MS platform for research in pharmacology and medicinal chemistry. Natural Sciences and Engineering Research Council (Ottawa, Canada). 147 201 $. (2012-2013).
  Numéro de subvention : grant.2861480. Voir plus
• Subvention. The apoptotic caspase proteolytic system. Natural Sciences and Engineering Research Council (Ottawa, Canada). 155 000 $. (2010-2015).
  Numéro de subvention : 355388-2010. Voir plus
• Subvention. Mechanisms of apoptotic caspases activation. Canada Foundation for Innovation (Ottawa, Canada). 127 781 $. (2008).
  Numéro de subvention : 18637. Voir plus
• Subvention. Mécanisme d'activation des caspases apoptotiques. Fonds de Recherche du Québec - Santé (Montreal, Canada). 30 000 $. (2008-2011).
  Numéro de subvention : 5852. Voir plus
• Subvention. Mécanisme d'activation des caspases apoptotiques. Fonds de Recherche du Québec - Santé (Montreal, Canada). 204 493 $. (2008-2012).
  Numéro de subvention : 13934. Voir plus
• Subvention. Mecanismes d'activation des caspases apoptotiques. Canadian Institutes of Health Research (Ottawa, Canada). 572 492 $. (2008-2013).
  Numéro de subvention : 200709MOP. Voir plus
• Subvention. Special grant from the President's Fund in conjunction with the Banting Foundation to support your research efforts. Canadian Institutes of Health Research (Ottawa, Canada). 20 000 $. (2007-2008).
  Numéro de subvention : 200704MPF. Voir plus
• Subvention. Role of caspase-7 in colorectal cancer. Mitacs (Vancouver, Canada).
  Numéro de subvention : grant.13968142. Voir plus

Publications

Articles

• Frédérick Comtois, Jean-François Jacques, Lenna Métayer, Wend Yam DD Ouedraogo, Aïda Ouangraoua, Jean-Bernard Denault, Xavier Roucou. (2025). Noncanonical altPIDD1 protein: unveiling the true major translational output of thePIDD1gene. Life Science Alliance. DOI
• Laiyen Garcia Delgado, Amélie Derome, Samantha Longpré, Marilyne Giroux-Dansereau, Ghenwa Basbous, Christine Lavoie, Caroline Saucier, Jean-Bernard Denault. (2024). Spatiotemporal regulation of the hepatocyte growth factor receptor MET activity by sorting nexins 1/2 in HCT116 colorectal cancer cells. Bioscience Reports. DOI
• Le Moal E, Liu Y, Collerette-Tremblay J, Dumontier S, Fabre P, Molina T, Dort J, Orfi Z, Denault N, Boutin J, Michaud J, Giguère H, Desroches A, Trân K, Ellezam B, Vézina F, Bedard S, Raynaud C, Balg F, ... Bentzinger CF. (2024). Apelin stimulation of the vascular skeletal muscle stem cell niche enhances endogenous repair in dystrophic mice. Science translational medicine. DOI
• Alexandre Desroches, Jean-Bernard Denault. (2021). Characterization of caspase-7 interaction with RNA. Biochemical Journal. DOI
• Alexandre Desroches, Jean-Bernard Denault. (2019). Caspase-7 uses RNA to enhance proteolysis of poly(ADP-ribose) polymerase 1 and other RNA-binding proteins. Proceedings of the National Academy of Sciences. DOI
• Marjan Gharagozloo, Shaimaa Mahmoud, Camille Simard, Kenzo Yamamoto, Diwakar Bobbala, Subburaj Ilangumaran, Matthew D. Smith, Albert Lamontagne, Samir Jarjoura, Jean-Bernard Denault, Véronique Blais, Louis Gendron, Carles Vilariño-Güell, A. Dessa Sadovnick, Jenny P. Ting, Peter A. Calabresi, Abdelaziz Amrani, Denis Gris. (2019). NLRX1 inhibits the early stages of CNS inflammation and prevents the onset of spontaneous autoimmunity. PLOS Biology. DOI
• Lemieux MJ, Denault JB, Overall CM. (2018). Highlight: Frontiers in Proteolysis. Biological chemistry. DOI
• Cyrielle Martini, Mikaël Bédard, Pierre Lavigne, Jean-Bernard Denault. (2017). Characterization of Hsp90 Co-Chaperone p23 Cleavage by Caspase-7 Uncovers a Peptidase–Substrate Interaction Involving Intrinsically Disordered Regions. Biochemistry. DOI
• Duclos C, Lavoie C, Denault JB. (2017). Caspases rule the intracellular trafficking cartel. The FEBS journal. DOI
• Duclos CM, Champagne A, Carrier JC, Saucier C, Lavoie CL, Denault JB. (2017). Caspases play in traffic. Cell death & disease. DOI
• Duclos CM, Champagne A, Carrier JC, Saucier C, Lavoie CL, Denault JB. (2017). Caspase-mediated proteolysis of the sorting nexin 2 disrupts retromer assembly and potentiates Met/hepatocyte growth factor receptor signaling. Cell death discovery. DOI
• Mélissa Lessard-Beaudoin, Mélissa Laroche, Amal Loudghi, Marie-Josée Demers, Jean-Bernard Denault, Guillaume Grenier, Sean-Patrick Riechers, Erich E. Wanker, Rona K. Graham. (2016). Organ-specific alteration in caspase expression and STK3 proteolysis during the aging process. Neurobiology of Aging. DOI
• Lessard-Beaudoin M, Laroche M, Demers MJ, Duclos C, Denault JB, Grenier G, Riechers SP, Wanker EE, Graham RK. (2016). Age-dependent differential expression of death-associated protein 6 (Daxx) in various peripheral tissues and different brain regions of C57BL/6 male mice. Biogerontology. DOI
• Jean-Sébastien Maltais, Elie Simard, Ulrike Froehlich, Jean-Bernard Denault, Louis Gendron, Michel Grandbois. (2016). iRAGE as a novel carboxymethylated peptide that prevents advanced glycation end product-induced apoptosis and endoplasmic reticulum stress in vascular smooth muscle cells. Pharmacological Research. DOI
• Boucher D, Duclos C, Denault JB. (2014). General in vitro caspase assay procedures. Methods in molecular biology (Clifton, N.J.). DOI
• Maltais JS, Denault JB, Gendron L, Grandbois M. (2012). Label-free monitoring of apoptosis by surface plasmon resonance detection of morphological changes. Apoptosis : an international journal on programmed cell death. DOI
• Elkoreh G, Blais V, Béliveau E, Guillemette G, Denault JB. (2012). Type 1 inositol-1,4,5-trisphosphate receptor is a late substrate of caspases during apoptosis. Journal of cellular biochemistry. DOI
• Boucher D, Blais V, Denault JB. (2012). Caspase-7 uses an exosite to promote poly(ADP ribose) polymerase 1 proteolysis. Proceedings of the National Academy of Sciences of the United States of America. DOI
• Boucher D, Blais V, Drag M, Denault JB. (2011). Molecular determinants involved in activation of caspase 7. Bioscience reports. DOI
• Arguin G, Caron AZ, Elkoreh G, Denault JB, Guillemette G. (2010). The transcription factors NFAT and CREB have different susceptibilities to the reduced Ca2+ responses caused by the knock down of inositol trisphosphate receptor in HEK 293A cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. DOI
• Scott FL, Fuchs GJ, Boyd SE, Denault JB, Hawkins CJ, Dequiedt F, Salvesen GS. (2008). Caspase-8 cleaves histone deacetylase 7 and abolishes its transcription repressor function. The Journal of biological chemistry. DOI
• Denault JB, Salvesen GS. (2008). Apoptotic caspase activation and activity. Methods in molecular biology (Clifton, N.J.). DOI
• Timmer JC, Enoksson M, Wildfang E, Zhu W, Igarashi Y, Denault JB, Ma Y, Dummitt B, Chang YH, Mast AE, Eroshkin A, Smith JW, Tao WA, Salvesen GS. (2007). Profiling constitutive proteolytic events in vivo. The Biochemical journal. DOI
• Denault JB, Drag M, Salvesen GS, Alves J, Heidt AB, Deveraux Q, Harris JL. (2007). Small molecules not direct activators of caspases. Nature chemical biology. DOI
• Truscott M, Denault JB, Goulet B, Leduy L, Salvesen GS, Nepveu A. (2007). Carboxyl-terminal proteolytic processing of CUX1 by a caspase enables transcriptional activation in proliferating cells. The Journal of biological chemistry. DOI
• Denault JB, Eckelman BP, Shin H, Pop C, Salvesen GS. (2007). Caspase 3 attenuates XIAP (X-linked inhibitor of apoptosis protein)-mediated inhibition of caspase 9. The Biochemical journal. DOI
• Denault JB, Békés M, Scott FL, Sexton KM, Bogyo M, Salvesen GS. (2006). Engineered hybrid dimers: tracking the activation pathway of caspase-7. Molecular cell. DOI
• Berger AB, Witte MD, Denault JB, Sadaghiani AM, Sexton KM, Salvesen GS, Bogyo M. (2006). Identification of early intermediates of caspase activation using selective inhibitors and activity-based probes. Molecular cell. DOI
• Simonovic M, Denault JB, Salvesen GS, Volz K, Gettins PG. (2005). Lack of involvement of strand s1'A of the viral serpin CrmA in anti-apoptotic or caspase-inhibitory functions. Archives of biochemistry and biophysics. DOI
• Scott FL, Denault JB, Riedl SJ, Shin H, Renatus M, Salvesen GS. (2005). XIAP inhibits caspase-3 and -7 using two binding sites: evolutionarily conserved mechanism of IAPs. The EMBO journal. DOI
• Bruckner S, Rhamouni S, Tautz L, Denault JB, Alonso A, Becattini B, Salvesen GS, Mustelin T. (2005). Yersinia phosphatase induces mitochondrially dependent apoptosis of T cells. The Journal of biological chemistry. DOI
• Boatright KM, Deis C, Denault JB, Sutherlin DP, Salvesen GS. (2004). Activation of caspases-8 and -10 by FLIP(L). The Biochemical journal. DOI
• Denault JB, Boatright K. (2004). Apoptosis in Biochemistry and Structural Biology. 3-8 February 2004, Keystone, CO, USA. IDrugs : the investigational drugs journal.
• Denault JB, Salvesen GS. (2003). Human caspase-7 activity and regulation by its N-terminal peptide. The Journal of biological chemistry. DOI
• Denault JB, Salvesen GS. (2003). Expression, purification, and characterization of caspases. Current protocols in protein science. DOI
• Denault JB, Salvesen GS. (2002). Caspases: keys in the ignition of cell death. Chemical reviews. DOI
• Blais V, Fugère M, Denault JB, Klarskov K, Day R, Leduc R. (2002). Processing of proendothelin-1 by members of the subtilisin-like pro-protein convertase family. FEBS letters. DOI
• Denault JB, Salvesen GS. (2002). Caspases. Current protocols in protein science. DOI
• Dufour EK, Denault JB, Bissonnette L, Hopkins PC, Lavigne P, Leduc R. (2001). The contribution of arginine residues within the P6-P1 region of alpha 1-antitrypsin to its reaction with furin. The Journal of biological chemistry. DOI
• Bush G, diSibio G, Miyamoto A, Denault JB, Leduc R, Weinmaster G. (2001). Ligand-induced signaling in the absence of furin processing of Notch1. Developmental biology. DOI
• Denault JB, Lazure C, Day R, Leduc R. (2000). Comparative characterization of two forms of recombinant human SPC1 secreted from Schneider 2 cells. Protein expression and purification. DOI
• Lora M, Denault JB, Leduc R, de Brum-Fernandes AJ. (1998). Systematic pharmacological approach to the characterization of NSAIDs. Prostaglandins, leukotrienes, and essential fatty acids. DOI
• Dufour EK, Denault JB, Hopkins PC, Leduc R. (1998). Serpin-like properties of alpha1-antitrypsin Portland towards furin convertase. FEBS letters. DOI
• Kido T, Sawamura T, Hoshikawa H, D'Orléans-Juste P, Denault JB, Leduc R, Kimura J, Masaki T. (1997). Processing of proendothelin-1 at the C-terminus of big endothelin-1 is essential for proteolysis by endothelin-converting enzyme-1 in vivo. European journal of biochemistry. DOI
• Mains RE, Berard CA, Denault JB, Zhou A, Johnson RC, Leduc R. (1997). PACE4: a subtilisin-like endoprotease with unique properties. The Biochemical journal. DOI
• Denault JB, Leduc R. (1996). Furin/PACE/SPC1: a convertase involved in exocytic and endocytic processing of precursor proteins. FEBS letters. DOI
• Denault JB, Claing A, D'Orléans-Juste P, Sawamura T, Kido T, Masaki T, Leduc R. (1995). Processing of proendothelin-1 by human furin convertase. FEBS letters. DOI
• Denault JB, D'Orléans-Juste P, Masaki T, Leduc R. (1995). Inhibition of convertase-related processing of proendothelin-1. Journal of cardiovascular pharmacology.
• Vidricaire G, Denault JB, Leduc R. (1993). Characterization of a secreted form of human furin endoprotease. Biochemical and biophysical research communications. DOI
• Laporte S, Denault JB, D'Orléans-Juste P, Leduc R. (1993). Presence of furin mRNA in cultured bovine endothelial cells and possible involvement of furin in the processing of the endothelin precursor. Journal of cardiovascular pharmacology. DOI

Autres contributions

Cours enseignés à l'UdeS

• PHR102 - Principes de pharmacologie I. (2023-2025). (3CR).
• PHR501 - Pharmacologie moléculaire. (2023). (3CR).

Présentations

• Jean-Bernard Denault. (2023). BRET biosensors for kinetic analysis of caspase activity. Intl. Proteolysis Society – 12th General Meeting.
• Jean-Bernard Denault. (2018). The N-terminal domain of caspase-7 is an intrinsically disordered region that selects key substrates. GRC on Proteolytic Enzymes & Their Inhibitors.
• Jean-Bernard Denault. (2018). Cleavage of SNX2 by caspases promotes hepatocyte growth factor receptor signalling in colorectal cancer. Bioscience Reports Symposium.
• Jean-Bernard Denault. (2016). Caspase-mediated proteolysis of SNX1 and SNX2 proteins. GRC on Proteolytic Enzymes & Their Inhibitors.
• Jean-Bernard Denault. (2006). Engineered hybrid dimers: tracking the activation pathway of caspase-7. Pacific Coast Protease Workshop.
• Jean-Bernard Denault. (2005). Caspase-7 activation through sequential cleaved of its linker region: a refined model for executioner caspase activation. Intl. Proteolysis Society.
• Jean-Bernard Denault. (2005). Half cleavage for Half Moon Bay is all you need. Pacific Coast Protease Workshop.

Activités professionnelles

• Université de Sherbrooke. Pharmacology & Physiology. Directeur/Department Head. Sherbrooke, Quebec, Canada. (2024-).
• Université de Sherbrooke. Pharmacology & Physiology. Dir. Bachelor's Degree in Pharmacology. Sherbrooke, Quebec, Canada. (2013-).
• Université de Sherbrooke. Pharmacology & Physiology. Dir. graduate Programs in Pharmacology. Sherbrooke, Quebec, Canada. (2010-2013).