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Menendez, Alfredo

Professeur, Faculté de médecine et des sciences de la santé
FMSS Dép. de microbiologie et infectiologie

Coordonnées

Courriel

819-821-8000, poste 75326

Diplômes

(2005) Epitope-targeting strategy for a vaccine against HIV-1: Peptide ligands for the broadly-neutralizing antibodies b12, 2F5 and 2G12. Doctorate (Doctor of Philosophy). Simon Fraser University.

(1985) Proteinas plasmaticas y variabilidad ambiental en tres especies del genero Anolis. Bachelor's (Licenciado en Ciencias Biologicas). University of Havana-Cuba.

Présentation

Sujets de recherche

Bacterial Infections, Cell Signaling and Infectious and Immune Diseases, Endocrine System, Gastrointestinal Pathologies, Glands and Tissues, Hepatic Diseases, Immune Mediators: Cytokines and Chemokines, Immune System, Infectious Diseases, Microorganisms.

Disciplines de recherche

Biochemistry, Immunology, Microbiology, Molecular Biology.

Mots-clés

Bacterial infections, Enteroendocrine hormones, Enterohepatic pathogenesis, Hepatobiliary system, HIV-1 vaccines/immunology, Immunometabolism, Inflammation, Innate immunity, Intestinal antimicrobial peptides and proteins, Intestinal defenses.

Intérêts de recherche

I am interested in gastrointestinal and hepatobiliary immunology, microbiology and endocrinology. I study the molecular mechanisms of gut-liver communication in the homeostasis and immunity of the enterohepatic system, with an emphasis in the role of commensal and pathogenic bacteria in these fundamental processes.

Centre de recherche

Centre de recherche du CHUS

Langues parlées et écrites

Anglais, Français, Espagnol (castillan)

Publications

Articles de revue

  • G. Romain, S. Tremblay, E. T. Arena, L. C. M. Antunes, S. Covey, M. T. Chow, B. B. Finlay & A. Menendez. (2013). Enterohepatic bacterial infections dysregulate the FGF15-FGFR4 endocrine axis. BMC Microbiology, 13(238), 1-10. (Published).
  • Menendez A, Willing BP, Montero M, Wlodarska M, So CC, Bhinder G, Vallance BA and Finlay BB. (2013). Bacterial stimulation of the TLR-MyD88 pathway modulates the homeostatic expression of ileal Paneth cells alpha-defensins. Journal of Innate Immunity, 5(1), 39-49. (Published).
  • Arena ET, Auweter SD, Antunes LC, Vogl AW, Han J, Guttman JA, Croxen MA, Menendez A, Covey SD, Borchers CH, Finlay BB. (2011). The deubiquitinase activity of the Salmonella Pathogenicity Island 2 effector, SseL, prevents accumulation of cellular lipid droplets. Infection and Immunity, 79, 4392-4400. (Published).
  • Antunes LC, Andersen SK, Menendez A, Arena ET, Han J, Ferreira RB, Borchers CH, Finlay BB. (2011). Metabolomics reveals phospholipids as important nutrient sources during Salmonella growth in bile in vitro and in vivo. Journal of Bacteriology, 193, 4719-4725. (Published).
  • Wlodarska, M., Willing, B., Keeney, K.M., Menendez, A., Bergstrom, K.S., Gill, K., Russell, S.L., Vallance, B.A. and Finlay, B.B. (2011). Antibiotic treatment alters the colonic mucus layer and predisposes the host to exacerbated Citrobacter rodentium-induced colitis. Infection and Immunity, 79(4), 1536-1545. (Published).
  • L. Caetano M. Antunes, Ellen T. Arena, Alfredo Menendez, Jun Han, Rosana B. R. Ferreira, Michelle M. Buckner, Petra Lolić, Christoph H. Borchers and B. Brett Finlay. (2011). The impact of Salmonella infection on host hormone metabolism revealed by metabolomics. Infection and Immunity, 79, 1759-1769. (Published).
  • Irving, M.B., Craig, L., Menendez, A., van Houten, N.E., Montero, M. and Scott, J.K. (2010). Exploring Peptide Mimics for the Production of Antibodies Against Discontinuous Protein Epitopes. Molecular Immunology, 47, 1137-1148. (Published).
  • Menendez, A., Ferreira RB. & Finlay BB. (2010). Defensins keep the peace too. Nature Immunology, 11, 49-50. (Published).
  • Menendez, A., Arena, ET., Guttman, JA., Thorson, L., Vallance, BA., Vogl, W. & Finlay BB. (2009). Salmonella infection of gallbladder epithelial cells drives local inflammation and injury in a model of acute typhoid fever. The Journal of Infectious Diseases, 200(11), 1703-1713. (Published).
  • O.L. Champion, Y. Valdez, L. Thorson, J.A. Guttman, A. Menendez, E.C. Gaynor & B.B. Finlay. (2008). A murine intraperitoneal infection model reveals that host resistance to Campylobacter jejuni is Nramp1 dependent. Microbes and Infection, 10, 922-927. (Published).
  • A. Menendez, D. A. Calarese, R. L. Standfield, K. C. Chow, C. N. Scanlan, R. Kunert, H. Katinger, D. R. Burton, I. A. Wilson & J.K. Scott. (2008). A peptide inhibitor of HIV-1 neutralizing antibody 2G12 is not a structural mimic of the natural carbohydrate epitope on gp120. The FASEB Journal, 22, 1380-1392. (Published).
  • A. Menendez & B. Brett Finlay. (2007). Defensins in the immunology of bacterial infections. Current Opinion in Immunology, 19, 385-391. (Published).
  • E. Ollmann Saphire, M. Montero, A. Menendez, N. E.van Houten, R. Pantophlet, M. B. Zwick, P. W.H.I. Parren, D. R. Burton, J. K. Scott & I. A. Wilson. (2007). Structure of a high-affinity "mimotope" peptide bound to HIV-1-neutralizing antibody b12 explains its inability to elicit gp120 cross-reactive antibodies. Journal of Molecular Biology, 369, 696-709. (Published).
  • van Houten, N.E., Zwick, M.B., Menendez, A., and Scott, J.K. (2006). Filamentous phage as an immunogenic carrier to elicit focused antibody responses against a synthetic peptide. Vaccines, 24, 4188-4200. (Published).
  • A. Menendez and J. K. Scott. (2005). The Nature of Target-Unrelated Peptides Recovered in the Screening of Phage-Displayed Random Peptide Libraries with Antibodies. Analytical Biochemistry, 336, 145-157. (Published).
  • A. Menendez, K. C. Chow, O. C. C. Pan and J. K. Scott. (2004). Human Immunodeficiency Virus type 1-Neutralizing Monoclonal Antibody 2F5 is Multispecific for Sequences Flanking the DKW Core Epitope. Journal of Molecular Biology, 338, 311-327. (Published).
  • Zwick, M.B., Bonnycastle, L.L., Menendez, A., Parren, P.W.H.I., Irving, M.B., Barbas, C.F. III, Burton, D.R. and Scott, J.K. (2001). Identification and Characterization of a Peptide That Specifically Binds the Human, Broadly Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibody b12. Journal of Virology 75:6692-6699, 2001, 75, 6692-6699. (Published).
  • M. Montero, A. Menendez, M. Dominguez, L. Navea, O. Vilarubia, D. Quintana, M. Izquierdo, V. Jimenez, O. Reyes, L. Lobaina, E. Noa and C. Duarte. (1997). Broadly Reactive Antibodies Against a gp120 V3 loop Multi-Epitope Polypeptide Neutralize Different Isolates of Human Immunodeficiency Virus Type 1. Vaccine 15:1200-1208, 1997., 15, 1200-1208. (Published).
  • B. Gonzalez, O. Garcia, A. Sosa, J. Fernandez and A. Menendez. (1996). The argU Gene Enhances the Expression of Human α-2 IFN in E. coli. Annals of the New York Academy of Sciences, 782, 79-86. (Published).
  • Duarte, M. Montero, A. Seralena, R. Valdes, V. Jimenez, J. Benitez, E. Narciandi, J. Madrazo, G. Padron, G. Sanchez, G. Girlund, K. Pearson, M. Dominguez, B. Wharen, and A. Menendez. (1994). Multiepitope polypeptide of the HIV-1 envelope induces neutralizing monoclonal antibodies against V3 loop. AIDS Research and Human Retroviruses 10:235-243,1994., 10, 235-243. (Published).
  • M. Ayala, M. Duenas, A. Santos, A. Menendez, A. Silva and J. Gavilondo. (1992). Bacterial Single-Chain Antibody Fragments, Specific for Carcinoembrionic Antigen. BioTechniques, 13, 790-799. (Published).
  • D. Bobbala, M. Mayhue, A. Menendez, S. Ilangumaran & S. Ramanathan. Trans-presentation of IL-15 by IL-15Rα is dispensablefor the pathogenesis of autoimmune type 1 diabetes. Cellular & Molecular Immunology, (Accepted).

Chapitres de livre

  • Zwick, M.B., A. Menendez, L.L. Bonnycastle and J.K. Scott. (2001). Analysis of Phage-borne Peptides. Phage Display: A laboratory manual. (18.1-18.44). Cold Spring Harbor Laboratory Press. (Published).
  • Bonnycastle, L.L., A. Menendez and J.K. Scott. (2001). General Phage Methods. Phage Display: A laboratory manual. (15.1-15.30). Cold Spring Harbor Laboratory Press. (Published).
  • Bonnycastle, L.L., J. Shen, A. Menendez and J.K. Scott. (2001). Production of Peptide Libraries. Phage Display: A laboratory manual (16.1-16.28). Cold Spring Harbor Laboratory Press. (Published).
  • Menendez A, Bonnycastle L, Pan O, Scott J. (2001). Screening Peptide Libraries. Phage Display: A laboratory manual (17.1-17.32). Cold Spring Harbor Laboratory Press, New York. (Published).

Articles de conférence

  • S. Tremblay, G. Grenier and A. Menendez. (2015). Antimicrobial peptide synthesis in the aged small intestine: its relationship with the commensal microbiota and epithelial integrity. Canadian Journal of Gastroenterology 29 (Supp A), 08A. (Published).
  • Sarah Tremblay, Guillaume Romain, Melisange Roux, Kirsty Brown, Xi-Lin Chen, Deanna Gibson, Sheela Ramanathan and Alfredo Menendez. (2015). Chenodeoxycholic acid induces the synthesis of intestinal antimicrobial peptides through activation of the STAT3 signaling pathway. Canadian Journal of Gastroenterology 29 (Supp A), 108A. (Published).
  • C. Beaurivage, A. Simoneau, S. St-Jean, S. Tremblay, S. Boudjadi, J. Allaire, S. Roy, J. Carrier, F. Boudreau, A. Menendez, N. Perreault, S. Ilangumaran and C. Saucier. (2015). The role of SOCS1 in intestinal homeostasis and colorectal carcinogenesis. Canadian Journal of Gastroenterology 29 (Supp A), 180A. (Published).
  • Sarah Tremblay, Guillaume Romain, Melisange Roux and Alfredo Menendez. (2014). Bile acids control Paneth cells antimicrobial peptides expression. Canadian Journal of Gastroenterology 28 (Supp A), 143A. (Published).
  • Martin Stahl, Sarah Tremblay, Wayne Vogl, Alfredo Menendez, Bruce Vallance. (2013). The defining role of Muc2 in Paneth cell granule secretion. 16th International Congress of Mucosal Immunity (ICMI 2013). (Published).
  • Guillaume Romain, Sarah Tremblay, Ellen T. Arena, Caetano Antunes, Scott Covey, Melisange Roux, Michael T. Chow, B. Brett Finlay and Alfredo Menendez. (2013). Dysfunctional FGF15/FGFr4 axis during bacterialinfections of the enterohepatic system. Canadian Digestive Diseases Week. (Published).
  • Sarah Tremblay, Guillaume Romain, Melisange Roux and Alfredo Menendez. (2013). The effects of bile acids on Paneth cells antimicrobialpeptides expression. Canadian Journal of Gastroenterology 27 (Supp A), 087A. (Published).
  • Alfredo Menendez, Ellen T. Arena, Julian Guttman, Lisa Thorson, Bruce Vallance, Wayne Vogl and B. Brett Finlay. (2008). Salmonella enterica serovar Typhimurium target gallbladderepithelial cells . 58th Annual Conference, Canadian Society of Microbiology. (Published).

Autres contributions

Cours enseignés

  • Sujets choisis en biologie moléculaire. MCR710. (2015-02-25).
  • Sujets choisis en biologie moléculaire. MCR710. (2012-02-29).
  • Molecular Virology. BIOL-312. (2010-03-18).
  • Molecular Immunology. MBB426. (2003-01-13).
  • Molecular Physiology. MBB322. (2001-09-10).

Présentations

  • (2015). Poster: Regulation of the Fibrogenic Response of Hepatic Stellate Cells by SOCS1. CIACCO 2015, Cytokines in Inflammation, Aging, Cancer and Obesity. Eastman.
  • (2015). Poster:Antimicrobial peptide synthesis in the aged small intestine: its relationship with the commensal microbiota and epithelial integrity. Canadian Digestive Diseases Week. Banff.
  • (2015). Poster:Chenodeoxycholic acid induces the synthesis of intestinal antimicrobial peptides through activation of the STAT3 signaling pathway. Canadian Digestive Diseases Week. Banff.
  • (2015). Poster: The role of SOCS1 in intestinal homeostasis and colorectal carcinogenesis. Canadian Digestive Diseases Week. Banff.
  • (2015). Invited presentation: Antimicrobial peptides in intestinal homeostasis. University of Sherbrooke, Dpmt. of Pediatrics (Immunology). Sherbrooke.
  • (2014). Poster: Bile acids control Paneth cells antimicrobial peptides expression. Canadian Digestive Diseases Week. Toronto.
  • (2013). Invited presentation: Bacteria in the enteroendocrine control of biliary metabolism. 2013 Annual Congress on Inflammation and Pain. Orford.
  • (2013). Poster: Inflammatory processes decrease the abundance of the FGF15 receptor complex. 10th Symposium on the Physiology and Diseases of the Digestive Tract. Orford.
  • (2013). Poster: The defining role of Muc2 in Paneth cell granule secretion. 16th International Congress of Mucosal Immunity (ICMI 2013). Vancouver.
  • (2013). Poster: Dysfunctional FGF15/FGFr4 axis during bacterial infections of the enterohepatic system. Canadian Digestive Diseases Week. Victoria.
  • (2012). Invited presentation: Paneth cells, microbes and defensins expression. University of Sherbrooke, Dpmt. of Anatomy and Cell Biology. Sherbrooke.
  • (2011). Invited presentation: Biliary function and enterohepatic bacterial infections. Department of Microbiology and Immunology, McGill University. Montreal.
  • (2011). Poster: Biliary function in the synthesis of intestinal antimicrobials. CIHR Institute of Infection and Immunity New Investigators Meeting. Lac Delage.
  • (2010). Invited presentation: The gallbladder in systemic Salmonella infection. University of North Dakota. Grand Forks.
  • (2010). Invited presentation: The impact of acute, systemic salmonellosis on the hepatobiliary system. UBC Okanagan. Kelowna.
  • (2008). Invited presentation: Bacteria in the gallbladder: The Salmonella case. Child & Family Research Institute (CFRI). Vancouver.
  • (2008). Invited presentation: Salmonella enterica serovar Typhimurium target gallbladder epithelial cells. 58th Annual Conference of the Canadian Society of Microbiologists. Calgary.
  • (2008). Poster: Salmonella enterica serovar Typhimurium target gallbladder epithelial cells in vivo. 58th Annual Conference, Canadian Society of Microbiologists. Calgary.
  • (2008). Poster: Salmonella enterica serovar Typhimurium target gallbladder epithelial cells. Banff Conference on Infectious Diseases 2008. Banff.
  • (2007). Poster: Biochemical Characteristics and Immunogenicity of a Peptide Ligand for Broadly-Neutralizing MAb 4E10. Keystone Symposium, HIV Vaccines: From Basic Research to Clinical Trials. Whistler.
  • (2005). Poster: Peptide Ligands for the HIV-1 Neutralizing Monoclonal Antibody 2G12. International Consortium on Anti-Virals. Peterborough.
  • (2005). Invited presentation: Peptide ligands for the neutralizing antibody 2G12. AIDS Vaccines 2005 International Conference. Montreal.
  • (2005). Poster: Evolution of the Antibody Response Against Envelope Proteins During HIV-1 Infection. AIDS Vaccines 2005 International Conference. Montreal.
  • (2005). Poster: Peptide Markers for the HIV-1 Neutralizing Antibody 4E10. AIDS Vaccine 2005 International Conference. Montreal.
  • (2005). Invited presentation: Rapporteur, Basic Sciences Track. 14th Annual Canadian Conference on HIV/AIDS Research. Vancouver.
  • (2005). Invited presentation: Isolation of peptide epitopes for the Human Immunodeficiency Virus type 1-neutralizing monoclonal antibody 2G12. 14th Annual Canadian Conference on HIV/AIDS Research. Vancouver.
  • (2005). Poster: Evolution of the antibody response against the envelope protein of HIV-1. CAHR’s 14th Annual Canadian Conference on HIV/AIDS Research. Vancouver.
  • (2004). Poster: Evolution of the Antibody Response of the Envelope Proteins during HIV-1 Infection. Keystone Symposia " HIV Vaccine Development: Progress and Prospects X8. Whistler.
  • (2003). Poster: Studies on the identification of the complete 2F5 epitope by the screening of phage displayed peptide libraries. Keystone Symposia "HIV Vaccine Development: Immunological and Biological Challenges. Banff.
  • (2002). Invited presentation: Peptide epitope mimics for the HIV-1-neutralizing antibodies b12, 2F5 and 2G12. The Scripps Research Institute. La Jolla.
  • (2002). Poster: A Peptide that Specifically Binds the Broadly anti-HIV-1 Neutralizing Antibody b12. Keystone Symposium. Keystone.
  • (2002). Poster: B2.1, a Peptide that Specifically Binds the Broadly HIV-1-Neutralizing, Human Antibody b12. 9th Conference on Retroviruses and Opportunistic Infections. Seattle.
  • (2001). Poster: B2.1, a Peptide that Specifically Binds the Broadly HIV-1-Neutralizing, Human Antibody, b12. AIDS Vaccine 2001. Philadelphia.
  • (2001). Poster: Filamentous Phage for Producing Antibody Responses Focused against B2.1, an HIV-1 Vaccine Lead Peptide. AIDS Vaccine 2001. Philadelphia.
  • (2001). Poster: Hen Eggwhite Lysozyme as a Model Antigen for Producing Epitope-Target Vaccines. AIDS Vaccine 2001. Philadelphia.
  • (2000). Poster: The Filamentous Phage as a Multivalent Scaffold for Coupling Synthetic Peptides for Immunization. Understanding Phage Display: Structure, Biology and Applications. Vancouver.
  • (1995). Invited presentation: Recombinant Multi-Epitope Polypeptides of HIV-1 Envelope as anti-HIV Vaccine Candidates. II International Congress on HIV infection and AIDS in Cuba. La Habana.