Wagner, Richard J.

Professeur, Faculté de médecine et des sciences de la santé
FMSS Dép. médecine nucléaire et radiobiologie

Coordonnées

Courriel


819-821-8000, poste 72717

Diplômes

(1992) Post-doctorate (Biochemistry and Molecular Biology). University of California, Berkeley.

(1989) Post-doctorate (Radiobiology). Université de Sherbrooke.

(1988) Doctorate (PhD in Chemistry). Université de Sherbrooke.

(1984) Master's Thesis (Radiobiology - Masters). Université de Sherbrooke.

(1980) Bachelor's (Chemistry). Dalhousie University.

Présentation

Sujets de recherche

Biotechnology, Carcinogenesis, Genomic Damage and Repair, Nucleic Acids.

Disciplines de recherche

Biochemistry, Chemistry.

Mots-clés

Antioxidants, Cancer, Chromatography, DNA damage, DNA repair, Mass spectrometry, Mutagenesis, Organic free radicals, Oxidative stress, Photochemistry, Radiation chemistry.

Intérêts de recherche

My aim is to understand the mechanism of DNA damage induced by biological free radicals and ionizing radiation. We apply this knowledge to determine the role of DNA damage and repair in biological processus related to cancer. The development of assays to measure oxidatively modified DNA in vivo is a priority.

Centre de recherche

Centre de recherche sur le vieillissement du CSSS-IUGS

Langues parlées et écrites

Anglais, Français

Publications

Articles de revue

  • Flandrin, A.; Allouche, S.; Rolland, Y.; McDuff, F.-O.; Wagner, J.R. and Klarskov, K. (2015). Characterization of dehydroascorbate mediated modification of glutaredoxin by mass spectrometry. Journal of Mass Spectrometry, 50, 1358-1366. (In Press).
  • *Ali, A and Wagner, J.R. (2015). Isomerization of 5-hydroxy-5-methylhydantoin 2’-deoxynucleoside into α-furanose, β-furanose, α-pyranose and β-pyranoseanomers. Chemical Research in Toxicology, (Revision Requested).
  • *Behmand, B.,Marignier, J.-L., Mostafavi, M., Wagner, J.R., Hunting, D.J., Sanche, L. (2015). Radiosensitization of DNA by Cisplatin Adducts Results from an Increase in the Rate Constant for the Reaction with Hydrated Electrons and Formation of PtI. Journal of Physical Chemistry B, 119(30), 9496-9500. (Published).
  • *Madugundu, G.S. and Wagner, J.R. (2015). Ozone-induced DNA damage: A pandora box of oxidatively modified DNA bases. Chemical Research in Toxicology, (Submitted).
  • Cadet, J and Wagner, J.R. (2014). Oxidatively generated base damage to cellular DNA by hydroxyl radical and one-electron oxidants: similarities and differences. Archives of Biochemistry and Biophysics, 557, 47-54. (Published).
  • Madugundu, G.S., Cadet, J. and Wagner, J.R. (2014). Hydroxyl-radical-induced oxidation of 5-methylcytosine in isolated and cellular DNA. Nucleic Acids Research, 42(11), 7450-7460. (Published).
  • Cadet J , Wagner JR , Shafirovich V , Geacintov NE. (2014). One-electron oxidation reactions of purine and pyrimidine bases in cellular DNA. International Journal of Radiation Biology, 90(6), 423-432. (Published).
  • Alizadeh, E., *Sanz, A. G., García, G., Wagner J. R. and Sanche L. (2014). Thymidine decomposition induced by low-energy electrons and soft X-rays under N2 and O2 atmospheres. Radiation Research, 181(6), 629-640. (Published).
  • *Behmand, B., Wagner, J.R., Sanche, L. and Hunting, D. J. (2014). Cisplatin intrastrand adducts sensitize DNA to base damage by hydrated electrons. Journal of Physical Chemistry B, 118(18), 4803-4808. (Published).
  • Cadet J , Wagner JR. (2014). TET enzymatic oxidation of 5-methylcytosine, 5-hydroxymethylcytosine and 5-formylcytosine. Mutation Research, 764-765, 18-35. (Published).
  • *Behmand B , Cloutier P , Girouard S , Wagner JR , Sanche L , Hunting DJ. (2013). Hydrated electrons react with high specificity with cisplatin bound to single-stranded DNA. The Journal of Physical Chemistry. B, 117(50), 15994-15999. (Published).
  • Kay P , Wagner JR , Gagnon H , Day R , Klarskov K. (2013). Modification of peptide and protein cysteine thiol groups by conjugation with a degradation product of ascorbate. Chemical Research in Toxicology, 26(9), 1333-1339. (Published).
  • Paquette B , Therriault H , Wagner JR. (2013). Role of interleukin-1β in radiation-enhancement of MDA-MB-231 breast cancer cell invasion. Radiation Research, 180(3), 292-298. (Published).
  • *Park Y , Peoples AR , *Madugundu GS , Sanche L , Wagner JR. (2013). Side-by-side comparison of DNA damage induced by low-energy electrons and high-energy photons with solid TpTpT trinucleotide. Journal of Physical Chemistry. B, 117(35), 10122-10131. (Published).
  • *Madugundu GS , Wagner JR , Cadet J , Kropachev K , Yun BH , Geacintov NE , Shafirovich V. (2013). Generation of Guanine-Thymine Cross-Links in Human Cells by One-Electron Oxidation Mechanisms. Chemical Research in Toxicology, 26(7), 1031-1033. (Published).
  • González-Magaña, O., Tiemens, M., Reitsma, G., Boschman, L., Door, M., Bari, S., Lahaie, P.O., Wagner, J.R., Huels, M.A., Hoekstra, R., Schlathölter, T. (2013). Fragmentation of protonated oligonucleotides by energetic photons and Cq+ ions. Physical Review A - Atomic, Molecular, and Optical Physics, 2013(87), Article 032702. (Published).
  • Cadet J., Douki T., Gasparutto D., Ravanat J.-L., Wagner J.R. (2012). Measurement of oxidatively generated base damage to nucleic acids in cells: facts and artifacts. Bioanalytical Review, 4, 55-74. (Published).
  • *Madugundu GS , *Park Y , Sanche L , Wagner JR. (2012). Radiation-induced formation of 2',3'-dideoxyribonucleosides in DNA: a potential signature of low-energy electrons. Journal of the American Chemical Society, 134(42), 17366-17368. (Published).
  • Meesat R , Belmouaddine H , Allard JF , Tanguay-Renaud C , Lemay R , Brastaviceanu T , Tremblay L , Paquette B , Wagner JR , Jay-Gerin JP , Lepage M , Huels MA , Houde D. (2012). Cancer radiotherapy based on femtosecond IR laser-beam filamentation yielding ultra-high dose rates and zero entrance dose. Proceedings of the National Academy of Sciences of the United States of America, 109(38), E2508-25013. (Published).
  • *Samson-Thibault F, *Madugundu GS, *Gao S, Cadet J, Wagner JR. (2012). Profiling cytosine oxidation in DNA by LC-MS/MS. Chemical Research in Toxicology, 25(9), 1902-1911. (Published).
  • *Park Y , *Polska K , Rak J , Wagner JR , Sanche L. (2012). Fundamental mechanisms of DNA radiosensitization: damage induced by low-energy electrons in brominated oligonucleotide trimers. Journal of Physical Chemistry. B, 116(32), 9676-9682. (Published).
  • *Desmarais G , Fortin D , Bujold R , Wagner JR , Mathieu D , Paquette B. (2012). Infiltration of glioma cells in brain parenchyma stimulated by radiation in the F98/Fischer rat model. International Journal of Radiation Biology, 88(8), 565--574. (Published).
  • Cadet J., Loft S., Olinski R., Evans M.D., Bialkowski K., Wagner J.R., Dedon P.C., Møller, P., Greenberg M.M., and Cooke M.S. (2012). Biologically relevant oxidants and terminology, classification and nomenclature of oxidatively generated damage to nucleobases and 2-deoxyribose in nucleic acids. Free Radical Research, 46, 367-381. (Published).
  • *Li Z , Cloutier P , Sanche L , Wagner JR. (2011). Low-energy electron-induced damage in a trinucleotide containing 5-bromouracil. Journal of Physical Chemistry. B, 115(46), 13668-13673. (Published).
  • Paquette B , Therriault H , *Desmarais G , Wagner R , Royer R , Bujold R. (2011). Radiation-enhancement of MDA-MB-231 breast cancer cell invasion prevented by a cyclooxygenase-2 inhibitor. British Journal of Cancer, 105(4), 534-541. (Published).
  • Khalil A , Gaudreau P , Cherki M , Wagner JR , Tessier DM , Fulop T , Shatenstein B. (2011). Antioxidant-rich food intakes and their association with blood total antioxidant status and vitamin C and E levels in community-dwelling seniors from the Quebec longitudinal study NuAge. Experimental Gerontology, 46(6), 475-481. (Published).
  • Defoy D , Dansette PM , Neugebauer W , Wagner JR , Klarskov K. (2011). Evaluation of deuterium labeled and unlabeled bis-methyl glutathione combined with nanoliquid chromatography-mass spectrometry to screen and characterize reactive drug metabolites. Chemical Research in Toxicology, 24(3), 412-417. (Published).
  • *Park Y., *Li Z., Cloutier P., Sanche L., Wagner J.R. (2011). Low energy electron induced DNA damage: Conversion of thymine to 5,6-dihydrothymine in the oligonucleotide trimer TpTpT. Radiation Research, 175, 240-246. (Published).
  • Regulus P , Desilets JF , Klarskov K , Wagner JR. (2010). Characterization and detection in cells of a novel adduct derived from the conjugation of glutathione and dehydroascorbate. Free Radical Biology and Medicine, 49(6), 984-991. (Published).
  • *Li Z , Cloutier P , Sanche L , Wagner JR. (2010). Low-energy electron-induced DNA damage: effect of base sequence in oligonucleotide trimers. Journal of the American Chemical Society, 132(15), 5422-5427. (Published).
  • Wagner JR , Cadet J. (2010). Oxidation reactions of cytosine DNA components by hydroxyl radical and one-electron oxidants in aerated aqueous solutions. Accounts of Chemical Research, 43(4), 564-571. (Published).
  • Cooke MS , Loft S , Olinski R , Evans MD , Bialkowski K , Wagner JR , Dedon PC , Møller P , Greenberg MM , Cadet J. (2010). Recommendations for standardized description of and nomenclature concerning oxidatively damaged nucleobases in DNA. Chemical Research in Toxicology, 23(4), 705-707. (Published).
  • *Kuttappan-Nair V , *Samson-Thibault F , Wagner JR. (2010). Generation of 2'-deoxyadenosine N6-aminyl radicals from the photolysis of phenylhydrazone derivatives. Chemical Research in Toxicology, 23(1), 48-54. (Published).

Chapitres de livre

  • Cadet J.; Wagner J.R. (2013). DNA Base Damage by Reactive Oxygen Species, Oxidizing Agents, and UV Radiation. DNA Repair, Mutagenesis, and Other Responses to DNA Damage. Cold Spring Harb Perspect Biol. (1-18). Cold Spring Harbor Laboratory Press. (Published).
  • Cadet, J., Douki, T., Gasparutto, D., Ravanat, J.-L. Wagner, J.R. (2012). Oxidatively Generated Nucleobase Modifications in Isolated and Cellular DNA. Encyclopedia of Radicals in Chemistry, Biology and Materials (3, 1319-1344). John Wiley and Sons, Inc. (Published).
  • Cadet, J., Douki, T., Gasparutto, D., Ravanat, J.-L. Wagner, J.R. (2010). Chemical reactions of the radical cations of nucleobases in isolated and cellular DNA. Formation of single-base lesions. Radical and Radical Ion Reactivity in Nucleic Acid Chemistry (Chapter 4). John Wiley & Sons, Inc. (Published).

Articles de conférence

  • *Choofong, S., Cloutier, P., Sanche, L. and Wagner, J.R. (2015). Radiation damage to DNA and oligonucleotides specifically induced by low energy electrons (LEEs) under atmospheric conditions. 61th annual meeting of the Radiation Research Society. (Published).
  • *Choofong, S., Cloutier, P. Sanche, L. and Wagner, J.R. (2015). Low energy electron (LEE)-induced damage to DNA. 98th Canadian Chemistry Conference and Exhibition. (Published).
  • *Madugundu, G.S. and Wagner, J.R. (2015). Oxidation of DNA by peroxyl radicals: Product analyses and mechanistic studies. 98th Canadian Chemistry Conference and Exhibition. (Published).
  • *Belmouaddine, H. *Madugundu, G.C., Wagner, J.R., Sanche, L., Houde, D. (2015). Low energy secondary electron-induced DNA damage through femtosecond laser pulse filamentation in aqueous solution. 15th international congress of radiation research. (Published).
  • Cadet,J. *Madugundu, G.S., Wagner, J.R. (2015). Radiation-induced damage to cytosine and 5-methylcytosine in cellular DNA: final oxidation products and mechanistic insights. 15th international congress of radiation research. (Published).
  • *Madugundu, G.S. and Wagner, J.R. (2014). Analysis of DNA oxidation in cells by LC-ESI-MS/MS. 27th Workshop on Tandem Mass Spectrometry. (Published).
  • *Lowry, C.M., Poirier, M.-B., Fortin, D. and Wagner, J.R. (2014). Alterations in Epigenetic Markers Related to 5-Hydroxymethylcytosine in Brain Cancer. 21st Annual Meeting of the Society for Free Radical Biology and Medicine. (Published).
  • Brochu, R., Rolland, Y., Wagner, J.R. and Klarskov, K. (2014). Possible relationship between oxidized vitamin C and homocysteine thiolactone: S-versus N-homocysteinylation. 21st Annual Meeting of the Society for Free Radical Biology and Medicine. (Published).
  • *B. Behmand, P. Cloutier, J. R. Wagner, L. Sanche, D. Hunting, J. L. Marignier, M. Mostafavi. (2014). Cisplatin AdductsSensitize DNA to Attack by Hydrated Electrons Leading to Cisplatin Detachmentand Base Damage. Annual Meeting of the Radiation Research Society. (Published).
  • *Behmand, B., Marignier, J.-L.; Mostafavi, M., Wagner, J.R., Hunting, D. and Sanche, L. (2014). Radiosentization of DNA by cisplatin adducts results from an increase in rate constant for the reaction with hydrated electrons and formation of Pt (I). 60th Annual Meeting of the Radiation Research Society. (Published).
  • *B. Behmand, P. Cloutier, S. Girouard, J. R. Wagner, L. Sanche, D. Hunting, J. L. Marignier, M. Mostafavi. (2014). Cisplatin Adducts Sensitize DNA to Attack by Hydrated Electrons Leading to Cisplatin Detachment and Base Damage. Gordon Research Conference on Radiation Chemistry. (Published).
  • Wagner, J.R. (2014). Profiling radiation-induced DNA damage. 13th Interational Workshop on Radiation Damage to DNA. (Published).
  • Alizadeh, E., *Sanz, A.G., Garcia, G., Wagner, J.R. and Sanche, L. (2014). Thymidine decomposition induced by low-energy electrons and soft X-rays under N2 and O2 atmospheres. 97th Canadian Chemistry Conference and Exhibition. (Published).
  • Wagner, J.R. (2013). La formation des lésions instables dans l’ADN exposé aux radiations. 11ème Colloque International de Radiobiologie Fondamentale et Appliquée. (Published).
  • Eloraby, G., McDuff, F.O.,Wagner, J.R., Klarskov,K. (2013). Ascorbylation and Glutathionylation of Glutaredoxin-1 by Dehydroascorbate. 10th International ISSX Meeting (International Society for the Study of Xenobiotics. (Published).
  • *Madugundu, G.S. and Wagner J. R. (2013). Oxidation of the methyl group of thymine and 5-methylcytosine in DNA: product analyses by LC-MS/MS and mechanistic studies. 61st ASMS Conference on Mass Spectrometry. (Published).
  • Klaus Klarskov; Aurore Flandrin; Francois-Olivier McDuff; J. Richard Wagner. (2013). Protein ascorbylation of human Glutaredoxin-1 by a reactive degradation product of oxidized ascorbate. 61st ASMS Conference on Mass Spectrometry. (Published).
  • *Gao, S. *Madugundu, G.S., Poirier, M.B., D. Fortin, Wagner, J.R. (2013). Analysis of 5‐Hydroxymethylcytosine as a Epigenetic Biomarker in Brain Cancer. 96th Canadian Chemistry Conference and Exhibition. (Published).
  • *Ali, A. and Wagner, J.R. (2013). Conversion of 5‐hydroxyl‐5‐methylhydantoin Nucleosides into a Mixture of Eight Isomers by Ring‐chain Tautomerism, Alpha/Beta and Furanose/Pyranose Isomerization. 96th Canadian Chemistry Conference and Exhibition. (Published).
  • Wagner, J. R. (2012). Oxidation of cytosine and 5-methylcytosine. 57th Annual Meeting of the Radiation Research Society. (Published).
  • *Madugundu, G.S., *Thibault-Samson, F., *Gao, S., Cadet, J., Wagner, J.R. (2012). Analysis of 5-methylcytosine oxidation products in DNA by enzymatic digestion and HPLC-MS/MS. 60th ASMS Conference on Mass Spectrometry. (Published).
  • *Kay, P.; Wagner, J.R.; Klarskov, K. (2012). Modification of peptide and protein cysteine thiol groups by reaction with oxidation products of vitamin C dehydroascorbate. 60th ASMS Conference on Mass Spectrometry. (Published).
  • *Kay, P., Wagner, J.R., Klarskov, K. (2011). Modification des thiols des cystéines des protéines et peptides en présence de déshydroascorbate. 79e Association canadienne-française pour l'avancement des sciences (ACFAS). (Published).
  • *Park, Y., Sanche, L., Wagner, J.R. (2011). Mécanisme de modification de la thymidine par irradiation à l’aide d’électrons de basse énergie sur l’oligonucléotide TpTpT. 79e Association canadienne-française pour l'avancement des sciences (ACFAS). (Published).
  • *Thibault-Samson, F., *Madugundu, G.S., Wagner, J.R. (2011). Oxydation de la cytosine dans l’ADN induits par les radicaux libres : Analyses des produits stables et les effets de la structure secondaire de l’ADN. 79e Association canadienne-française pour l'avancement des sciences (ACFAS). (Published).
  • *Park, Y.; *Li, Z.; Cloutier, P.; Sanche, L.; Wagner, J.R. (2010). Low energy electron induced DNA damage: Formation of thymine to 5,6-dihydrothymine as a major product in the irradiation of oligonucleotide trimer TpTpT. 56th Annual Meeting of the Radiation Research Society. (Published).
  • Wagner, J. R. (2010). Low energy electron induced DNA damage: chemical mechanisms. 56th Annual Meeting of the Radiation Research Society. (Published).
  • Defoy, D.; Dansette, P.M.; Neugebauer, W.; Wagner, J.R.; Klarskov, K. (2010). Evaluation of deuterium labeled and unlabeled bis-methyl glutathione combined with nanoliquid chromatography mass spectrometry to screen and characterize reactive drug metabolites. Biological Reactive Intermediates VIII. (Published).
  • Wagner, J. R. (2010). Oxidative damage to cytosine and 5-methylcytosine. EUCHEM Conference on Organic Free Radicals. (Published).
  • Meesat, R., Allard, J.-F., Wagner, J.R., Jay-Gerin, J.-P., Tremblay, L., Lepage, M. and Houde, D. (2009). Fricke and polymer gel dosimetry of femtosecond laser pulse filamentation and radiation chemical effects of laser irradiation on thymidine solutions. 55th Annual Meeting of the Radiation Research Society. (Published).
  • *Li, Z, Cloutier, P., Sanche, L. Wagner, J.R. (2009). Low Energy Electron Induced DNA Damage: Effect of Different Base Substitutions in Oligomer Trimers. 55th Annual Meeting of the Radiation Research Society. (Published).
  • Wagner, J.R. (2009). Induction of interstrand cross-links by photosensitization. 13th Congress of the European Society for Photobiology. (Published).
  • Wagner, J.R. *Nair, V.K., *Samson-Thibault, F., *Belmadoui, N. (2009). Photolysis of 2’-deoxyhydrazones: Analysis of products from the reaction of adenine N6-aminyl radicals. Organic Free Radicals. (Published).
  • Wagner, J.R.; *Peggy Regulus; *Jean-Francois Desilets; Klaus Klarskov. (2009). Conjugation of glutathione with oxidized ascorbate: Structural analysis of an adduct and its detection in cells. 57th ASMS Conference on Mass Spectrometry. (Published).
  • Wagner, J.R., *Belmadoui, N., Cadet, J. (2009). Free radical oxidation of cytosine and 5-methylcytoisine:Characterization of novel stable and unstable products. 8th Winter Research Conferences, Centre de l’École de Physique. (Published).
  • Cadet, J., Douki, T., Gasparrutto, D., Ravanat, J.-L., Wagner, J.R. (2009). Oxidatively generated damage to DNA: Recent mechanistic insights and perspectives. 8th Winter Research Conferences, Centre de l’École de Physique. (Published).

Autres contributions

Présentations

  • (2014). Profiling radiation-induced DNA damage. 13th Interational Workshop on Radiation Damage to DNA. Boston.
  • (2013). La formation des lésions instables dans l’ADN exposé aux radiations. 11ème Colloque International de Radiobiologie Fondamentale et Appliquée. La Grande Motte.
  • (2012). Oxidation of cytosine and 5-methylcytosine. 57th Annual Meeting of the Radiation Research Society. San Juan.
  • (2010). Low energy electron induced DNA damage: chemical mechanisms. 56th Annual Meeting of the Radiation Research Society. Wailea-Makena.
  • (2010). Oxidative damage to cytosine and 5-methylcytosine. EUCHEM Conference on Organic Free Radicals. Bologna.
  • (2010). Free radical oxidation of cytosine and 5-methylcytoisine:Characterization of novel stable and unstable products. 8th Winter Research Conferences, Centre de l’École de Physique, Les Houches, France. Les Houches.
  • (2009). Induction of interstrand cross-links by photosensitization. 13th Congress of the European Society for Photobiology. Wroclaw.
  • (2009). Photolysis of 2’-deoxyhydrazones: Analysis of products from the reaction of adenine N6-aminyl radicals. Organic Free Radicals-Tribute to Keith Ingold. Ottawa.

Les informations disponibles dans la base de données Expertus sont tirées du CV commun canadien.