Ryszard Brzezinski » Autres publications

Travaux en biologie moléculaire et enzymologie (suite)

Ghinet, M.G., Bordeleau, É., Brzezinski, R., Roy, S., Burrus, V. (2011) Uncovering the prevalence and diversity of Integrating Conjugative Elements in actinobacteria. PLOS One e27846. 

Brzezinski, R. Uncoupling chitosanase production from chitosan. (2011)  Bioengineered Bugs 2:4.

Dubeau, M. P., Poulin-Laprade, D., Ghinet, M.G., Brzezinski R. (2011) Properties of CsnR, the transcriptional repressor of the chitosanase gene csnA of Streptomyces lividans. Journal of Bacteriology 193:2441-2450.

Bernèche-d’Amours, A., Ghinet, M.G., Beaudin, J., Brzezinski, R., Roy, S. (2011) Sequence analysis of rpoB and rpoD gene fragments reveals the phylogenetic diversity of actinobacteria of genus Frankia. Canadian Journal of Microbiology 57:244-249.

Zitouni, M., Fortin, M., Thibeault, J.-S., Brzezinski, R. (2010)  A dye-labelled soluble substrate for the assay of endo-chitosanase activity. Carbohydrate Polymers 80:522-525.

Pluvinage, B., Ghinet, M. G., Brzezinski, R., Boraston, A. B., Stubbs, K. A. (2009) Inhibition of the exo-ß-D-glucosaminidase CsxA by a glucosamine-configured castanospermine and an amino-australine analogue. Organic & Biomolecular Chemistry 7:4169-4172.

Ghinet, M.G., Gagnon, J., Lacombe-Harvey, M.-È., Brzezinski, R. (2009) Molecular weight modulates the antimicrobial effect of chitosan on Escherichia coli. In: Rustichelli, F., Caramella, C., Senel, S., Varum, K. M. (Eds.) Advances in Chitin Science, vol. 11, pages 272 - 277, Venise, Italie.

Neugebauer, W.A., Brzezinski, R., Richter, M.V. (2009) Oligochitosan based multiple antigenic peptide. In: Rustichelli, F., Caramella, C., Senel, S., Varum, K. M. (Eds.) Advances in Chitin Science, vol. 11, pages 259 - 264, Venise, Italie.

Lacombe-Harvey, M.-È., Fukamizo, T.,  Gagnon, J., Ghinet, M.G., Dennhart, N., Letzel, T., Brzezinski, R. (2009) Accessory catalytic residues of chitosanase from Streptomyces sp. N174 - variations on a common theme in the lysozyme superfamily. FEBS Journal 276:857-869.

Dubeau, M.-P., Ghinet, M. G., Jacques, P.-É., Clermont, N., Beaulieu, C., Brzezinski, R. (2009) Cytosine deaminase as negative selection marker for gene disruption and replacement in the genus Streptomyces and other actinobacteria. Applied and Environmental Microbiology 75:1211-1214.

Van Bueren, A. L., Ghinet, M. G., Gregg, K., Fleury, A., Brzezinski, R., Boraston, A. B. (2009) The structural basis of substrate recognition in an exo-β-D-glucosaminidase involved in chitosan hydrolysis. Journal of Molecular Biology 385:131-139.

Dennhart, N., Fukamizo, T., Brzezinski, R., Lacombe-Harvey, M.-È., Letzel, T. (2008) Oligosaccharide hydrolysis by chitosanase enzymes monitored by real-time electrospray ionization - mass spectrometry. Journal of Biotechnology 134:253-260.

Roy, S., Fortin, M., Gagnon, J., Ghinet, M.G., LeHoux, J.-G., Dupuis, G. et Brzezinski, R. (2007). Quantitative fluorometric analysis of the protective effect of chitosan on thermal unfolding of catalytically active native and genetically-engineered chitosanases. Biochimica et Biophysica Acta 1774:975-984.

Fukamizo, T., Brzezinski, R. (2007) Structure and function of exo-ß-glucosaminidase from Amycolatopsis orientalis. (Review article) Journal of Applied Glycoscience. 54:133-138.

Fukamizo, T., Fleury, A., Côté, N., Mitsutomi, M., Brzezinski, R. (2006) Exo-β-D-glucosaminidase from Amycolatopsis orientalis: catalytic residues, sugar recognition specificity, kinetics, and synergism. Glycobiology 16:1064-1072.

Côté, N., Fleury, A., Dumont-Blanchette, É., Fukamizo, T., Mitsutomi, M.., Brzezinski, R. (2006) Two exo-β-D-glucosaminidases / exochitosanases from actinomycetes define a new subfamily of family 2 of glycoside hydrolases. Biochemical Journal 394:675-686.

Fukamizo, T., Katsumi, T., Lacombe-Harvey, M.-È., Tremblay, H., Brzezinski, R. (2005) Role of acidic amino acid residues in chitooligosaccharide-binding to Streptomyces sp. N174 chitosanase. Biochemical Biophysical Research Communications 338:1839-1844.

Li, H., Jacques, P.-É., Ghinet, M. G., Brzezinski, R., Morosoli, R. (2005) Determining the functionality of putative Tat-dependent signal peptides in Streptomyces coelicolor A3(2) by using two different reporter proteins. Microbiology 151:2189-2198.

LeHoux, J.G., Dupuis, G., Kelly, A., Brzezinski, R., Radwan F. (2005) Studies on the innocuousness of chitosan and its short-chain derivative Chitodextrin™ (LibracolTM) generated by enzymatic hydrolysis in human subjects. Dans : Struszczyk, H., Domard, A., Peter, M.G., Pospieszny, H. (eds) Advances in Chitin Science, vol. 8, pages 440-449.

Dubeau, M.-P., Broussau, S., Gervais, A., Masson, J.-Y., Brzezinski, R. (2005) A palindromic DNA sequence involved in the regulation of chitosanase gene expression in actinomycetes. Dans : Struszczyk, H., Domard, A., Peter, M.G., Pospieszny, H. (eds) Advances in Chitin Science vol. 8, pages 93-100.

Neugebauer, W.A., Lacombe-Harvey, M.-È., Gendron, G., Gobeil Jr. F., Brzezinski, R. (2005) Glycopeptide synthesis: chitosan oligosaccharides as modulators of biological activity of hormonal peptides. Dans : Struszczyk, H., Domard, A., Peter, M.G., Pospieszny, H. (eds) Advances in Chitin Science, vol. 8

Blanchard J., Park J.K., Boucher I. et Brzezinski R. 2003. Industrial applications of chitosanases. Dans: M. Fingerman and R. Nagabhushanam, ed. Recent Advances in Marine Biotechnology, vol.9, Biomaterials and Bioprocessing; Science Publishers, New Hampshire, pp. 257-277.

Brzezinski R., Côté N., Regoli D. et Neugebauer W.A. 2002. Enzymatically hydrolyzed chitosan as a carrier for presentation of bioactive peptides. In: Benedetti, E. and Pedone, C. (2002). Peptides 2002, pp. 444-445.

Lauzier A., Goyer C., Brzezinski R., Crawford D.L. et Beaulieu, C. 2002. Effect of amino acids on thaxtomin A biosynthesis in Streptomyces scabies. Canadian Journal of Microbiology 48:359-364.

Tremblay H., Yamaguchi T., Fukamizo T. et Brzezinski R. 2001. Mechanism of chitosanase-oligosaccharide interaction subsite structure of Streptomyces sp. N174 chitosanase and the role of Asp57 carboxylate. Journal of Biochemistry, 130:679-686.

Fayad K.P., Simao-Beaunoir A.-M., Gauthier A., Leclerc C., Mamady H., Beaulieu C. et Brzezinski R. 2001. Purification and properties of a beta-1,6 glucanase from Streptomyces sp. EF-14, an actinomycete antagonistic to Phytophthora spp. Applied Microbiology and Biotechnology, 57:117-123.

Blanchard J., Fukamizo T., Boucher I. et Brzezinski R. 2001. High level expression of the chitosanase from Streptomyces sp. N174 and study of its intramolecular interactions. Recent Research Developments in Microbiology, 5:111-121.

Fukamizo T., Juffer A.H. et Brzezinski R. 2001. A local structure assisting the enzyme catalysis from behind the catalytic center. Protein, Nucleic Acid and Enzyme, 46:1261-1267.

Fukamizo T., Juffer A.H., Vogel H.H., Honda Y., Tremblay H., Boucher I., Neugebauer W.A. et Brzezinski R. 2000. Theoretical calculation of pKa reveals an important role of Arg205 in the activity and stability of Streptomyces sp. N174 chitosanase. Journal of Biological Chemistry, 275:25633-25640.

Tremblay H., Blanchard J. et Brzezinski R. 2000. A common molecular signature unifies the chitosanases belonging to glycoside hydrolase families 46 and 80. Canadian Journal of Microbiology, 46:952-955.

Travaux en bio-informatique

Ntwari, A., Kelil, A., Drouin, R., Monga, E., Wang, S., Brzezinski, R., Bronsard, M., Yan, J. (2011) DNAc: a clustering method for identifying kinship relations between DNA profiles using a novel similarity measure. Journal of Forensic Sciences 56 (Suppl. 1):S17

Kelil, A., Wang, S., Jiang, Q., Brzezinski, R. (2010) A general measure of similarity for categorical sequences. Knowledge and Information Systems 24:197-220.

Kelil, A., Wang, S., Brzezinski, R. (2008) CLUSS2: an alignment-independent algorithm for clustering protein families with multiple biological functions. International Journal  of Computational Biology and Drug Design 1:122-140.

Kelil A, Wang S, Brzezinski R, Fleury A. CLUSS: Clustering of protein sequences based on a new similarity measure. BMC Bioinformatics 2007, 8:286.

Jacques, P.-É., Rodrigue, S., Gaudreau, L., Goulet, J., Brzezinski, R. (2006) Detection of prokaryotic promoters from the genomic distribution of hexanucleotide pairs. BMC Bioinformatics 7:423.

Jacques, P., Gervais, A., Cantin, M., Lucier, JF., Dallaire, G., Drouin, G., Gaudreau, L., Goulet, J., Brzezinski, R. 2005. MtbRegList, a database dedicated to the analysis of transcriptional regulation in Mycobacterium tuberculosis. Bioinformatics, 21:2563-2565.

Tremblay H., Blanchard J., Brzezinski R. 2000. Acommon molecular signature unifies the chitosanases belonging to glycoside hydrolase families 46 and 80. Canadian Journal of Microbiology, 46:952-955.

Travaux sur Mycobacterium tuberculosis

Rodrigue S., Brodeur J., Jacques P.-É., Gervais A.L., Brzezinski R., Gaudreau L. (2007).  Identification of Mycobacterial  s  Factor Binding Sites by Chromatin Immunoprecipitation Assays. Journal of Bacteriology 189:1505-1513

Dainese, E., Rodrigue, S., Delogu, G., Provvedi, R., Laflamme, L., Brzezinski, R., Fadda, G., Smith, I., Gaudreau, L., Palù, G., Manganelli, R. (2006) Posttranslational regulation of Mycobacterium tuberculosis extracytoplasmic-function sigma factor σL and roles in virulence and in global regulation of gene expression.  Infection and Immunity 74:2457-2461.

Jacques, J.-F., Rodrigue, S., Brzezinski, R., Gaudreau, L. (2006) A recombinant Mycobacterium tuberculosis in vitro transcription system. FEMS Microbiology Letters 255:140-147.

Beaucher J., Rodrigue S., Jacques P.-É., Smith I., Brzezinski R., Gaudreau L. 2002. Novel Mycobacterium tuberculosis anti-sigma factor antagonists control Sigma F activity by distinct mechanisms. Molecular Microbiology 45:1527-1540.

Travaux en biotechnologie environnementale

Girard, M., Viens, P., Avalos Ramirez, A., Brzezinski, R., Buelna, G., Heitz, M. (2012) Simultaneous treatment of methane and swine slurry by biofiltration. Journal of Chemical Technology & Biotechnology 87:697-704.

Veillette, M., Viens, P., Avalos Ramirez, A., Brzezinski, R., Heitz, M. (2011) Effect of ammonium concentration on microbial population and performance of a biofilter treating air polluted with methane. Chemical Engineering Journal 171:1114-1123.

Nikiema, J., Brzezinski, R., Heitz, M. (2010)The influence of phosphorus, potassium, and copper  on methane biofiltration performance. Canadian Journal of Civil Engineering 37:335-345.

Girard, M., Nikiema, J., Brzezinski, R., Buelna, G., Heitz, M. (2009) A review of the environmental pollution originating from the piggery industry and of the available mitigation technologies: towards the simultaneous biofiltration of swine slurry and methane. Canadian Journal of Civil Engineering 36:1946-1957.

Nikiema, J., Girard, M., Brzezinski, R., Heitz, M. (2009) Biofiltration of methane using an inorganic filter bed: Influence of inlet load and nitrogen concentration. Canadian Journal of Civil Engineering 36:1903-1910.

Jorio, H., Jin, Y., Elmrini, H., Nikiema, J., Brzezinski, R., Heitz, M. (2009) Treatment of VOCs in biofilters inoculated with fungi or microbial consortium. Environmental Technology 30:477-486.

Nikiema, J., Brzezinski, R., Heitz, M. (2007) Elimination of methane generated from landfills by biofiltration - a review. Reviews in Environmental Science and Bio/technology 6:241-284.

Prévost, K., Couture. G., Shipley, J. W., Brzezinski, R., Beaulieu, C. (2006) Effect of chitosan and a biocontrol streptomycete on field and potato tuber bacterial communities. Biocontrol 51:533-546.

Nikiema, J., Bibeau, L., Lavoie, J., Brzezinski, R., Vigneux, J., Heitz, M. (2005). Biofiltration of methane: an experimental study. Chemical Engineering Journal 113:111-117.

Jobin, G., Couture, G., Goyer, C., Brzezinski, R., Beaulieu, C. (2005) Streptomycete spores entrapped in chitosan beads as a novel tool for biocontrol of plant diseases. Applied Microbiology and Biotechnology 68:104-110.

Jorio, H., Bibeau, L., Brzezinski, R., Heitz, M. (2005) A novel procedure for the measurement of the kinetics of styrene biodegradation in a biofilter. Journal of Chemical Technology and Biotechnology 80:796-804.

Delhomenie, M.-C., Bibeau, L., Gendron, J., Brzezinski, R., Heitz, M. (2003) A study of clogging in a biofilter treating toluene vapors. Chemical Engineering Journal 94:211-222.

Roy S., Gendron J., Delhoménie M.-C., Bibeau L., Heitz M. et Brzezinski, R. 2003. Pseudomonas putida as the dominant toluene-degrading bacterial species during air decontamination by biofiltration. Applied Microbiology and Biotechnology 61:366-373.

Delhoménie M.-C., Bibeau L., Gendron J., Brzezinski R. et Heitz M. 2003. Degradation of toluene, xylene and trimethylbenzene vapors by biofiltration: a comparison. Journal of Air and Waste Management Association, 53:217-226.

Delhoménie M.-C., Bibeau L., Bredin N., Roy S., Broussau S., Brzezinski R.,  Kugelmass J.L. et  Heitz M. 2002. Biofiltration of air contaminated with toluene on a compost-based bed. Advances in Environmental Research, 6:239-254.

Roy S., Mysior P. et Brzezinski R. 2002. Comparison of dioxin and furan TEQ determination in contaminated soil using chemical, micro-EROD and immunoassay analysis. Chemosphere 48:833-842.

Delhoménie M.-C., Bibeau L., Gendron J., Brzezinski R. et Heitz M. 2001. Toluene removal by biofiltration influence of the nitrogen concentration on operational parameters. Industrial and Engineering Chemistry Research, 40:5405-5414.

Blanchard J., Akimov V.N., Neugebauer W.A., Brzezinski R. et Beaulieu C. 2001. Taxonomic study on two chitosanolytic strains isolated from soil. In R.A.A. Muzzarelli (editor) Chitin Enzymology 2001; ATEC Edizioni, Ancona, 263-271.

Côté N., Hogue R., Beaulieu C. et Brzezinski R. 2001. Suppressive effect of chitin waste-based compost on common scab of potato. In R.A.A. Muzzarelli (editor) Chitin Enzymology 2001 ; ATEC Edizioni, Ancona, 155-161.

Labrie C., Leclerc P., Côté N., Roy S., Brzezinski R., Hogue R. et Beaulieu C. 2001. Effect of chitin waste based composts produced by two-phase composting on two oomycete plant pathogens. Plant & Soil, 235:27-34.

Delhoménie M.-C., Bibeau L., Roy S., Brzezinski R. et Heitz M. 2001. Influence of nitrogen on the degradation of toluene contained in polluted air by biofiltration on a compost filter bed. Journal of Chemical Technology and Biotechnology, 76:997-1006.

Brevets d’invention

Brzezinski, R., Déry, C.V., Beaulieu, C., Éthier, J.-F., Harpin, S. Thermostable xylanase DNA, proteins and methods of use. Brevet USA N° 5871730 accordé en 2000. Brevets Canada nos 2129172 et 2154944 accordés en 2001 et 2002.

Brzezinski, R. Enzyme of use in chitosan hydrolysis. Brevet USA n° 5482843 accordé le 9 janvier 1996. Brevet Canada n° 2085292 accordé le 8 octobre 1999.

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Ryszard Brzezinski » Autres publications